Statistical optimization of production, purification and industrial application of a laundry detergent and organic solvent-stable subtilisin-like serine protease (Alzwiprase) from Bacillus subtilis DM-04

Sudhir, K.; Mukherjee, Ashis K.

doi:10.1016/j.bej.2009.09.007

Cited by 94 publications

(55 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The optimum pH value for the keratinolytic activity was 8.0. These results are in accordance with previous report showing that the keratinolytic protease produced by Bacillus species could be classified as an alkaline protease and was most active under neutral or alkaline conditions (Rai and Mukherjee 2010;Mazzoto et al 2010).…”

Section: Discussionsupporting

confidence: 95%

Characterization of a multifunctional feather-degrading Bacillus subtilis isolated from forest soil

et al. 2010

View full text Add to dashboard Cite

In this study, we isolated and characterized a novel feather-degrading bacterium that shows keratinolytic, antifungal and plant growth-promoting activities. A bacterium S8 was isolated from forest soil and confirmed to belong to Bacillus subtilis by BIOLOG system and 16S rRNA gene analysis. The improved culture conditions for the production of keratinolytic protease were 0.1% (w/v) sorbitol, 0.3% (w/v) KNO(3), 0.1% (w/v) K(2)HPO(4), 0.06% (w/v) KH(2)PO(4) and 0.04% (w/v) MgCl(2)·6H(2)O (pH 8.0 and 30°C), respectively. In the improved medium containing 0.1% (w/v) feather, keratinolytic protease production was around 53.3 ± 0.3 U/ml at 4 day; this value was 10-fold higher than the yield in the basal feather medium (5.3 ± 0.1 U/ml). After cultivation for 5 days in the improved medium, intact feather was completely degraded. Feather degradation resulted in free -SH group, soluble protein and amino acids production. The concentration of free -SH group in the culture medium was 15.5 ± 0.2 μM at 4 days. Nineteen amino acids including all essential amino acids were produced in the culture medium; the concentration of total amino acid produced was 3360.4 μM. Proline (2809.9 μM), histidine (371.3 μM) and phenylalanine (172.0 μM) were the major amino acids released in the culture medium. B. subtilis S8 showed the properties related to plant growth promotion: hydrolytic enzymes, ammonification, indoleacetic acid (IAA), phosphate solubilization, and broad-spectrum antimicrobial activity. Interestingly, the strain S8 grown in the improved medium produced IAA and antifungal activity, indicating simultaneous production of keratinolytic and antifungal activities and IAA by B. subtilis S8. These results suggest that B. subtilis S8 could be not only used to improve the nutritional value of feather wastes but also is useful in situ biodegradation of feather wastes. Furthermore, it could also be a potential biofertilizer or biocontrol agent applicable to crop plant soil.

show abstract

Section: Discussionsupporting

confidence: 95%

Characterization of a multifunctional feather-degrading Bacillus subtilis isolated from forest soil

et al. 2010

View full text Add to dashboard Cite

show abstract

“…It retained 74±2.6% of its original activity even after 1 h incubation at 37°C with SDS (5 mM). These results are in agreement with the reported proteases from various Bacillus species, showing stability in the presence of various surfactants (Oberio et al, 2001;Beg and Gupta, 2003;Kazan et al, 2005;Patel et al, 2006;Jaouadi et al, 2008;Rai and Mukherjee, 2010). The purified VSG-4 protease was detergent stable at room temperature which is necessary in laundry uses.…”

supporting

confidence: 95%

“…The obtained results clearly indicate that performance of enzymes in detergents depends on number of factors, including the detergents compounds since the proteolytic stability varied with each laundry detergents (Haddar et al, 2009). Therefore, partial loss of protease activity of VSG-4 in some of the detergents may be attributed to inhibitory effect(s) of component(s) of these detergents (Rai and Mukherjee, 2010).…”

mentioning

confidence: 89%

“…It has been recommended that proteases or the other hydrolytic enzymes to be used in detergent formulations should be effective at low levels ranging from 0.4% to 0.8% (Rai and Mukherjee, 2010) and therefore, it is reasonable to assume that VSG-4 is an ideal candidate for use in laundry detergent. Earlier reports made on addition of enzyme preparation with commercial detergent significantly enhanced the washing performances and removal of blood stains (Adinarayana et al, 2003;Arulmani et al, 2007;Rai and Mukherjee, 2010). The efficient stain removal capacity as well as the ability to retain the protease activity even in the presence of metal chelator (EDTA) and other surfactants could have a greater advantage of this enzyme in commercial laundry detergent formulations.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Purification and partial characterization of a detergent and oxidizing agent stable alkaline protease from a newly isolated Bacillus subtilis VSG-4 of tropical soil

et al. 2011

View full text Add to dashboard Cite

An extracellular detergent tolerant protease producing strain VSG-4 was isolated from tropical soil sample and identified as Bacillus subtilis based on morphological, biochemical characteristics as well as 16S-rRNA gene sequencing. The VSG-4 protease was purified to homogeneity using ammonium sulphate precipitation, dialysis and sephadex G-200 gel permeation chromatography with a 17.4 purification fold. The purified enzyme was active and stable over a broad range of pH (8.0-11.0, optimum at 9.0) and temperature (40°C to 60°C, optimum at 50°C). The thermostability of the enzyme was significantly increased by the addition CaCl(2). This enzyme was strongly inhibited by PMSF and DFP, suggesting that it belongs to the serine protease superfamily. The purified VSG-4 alkaline protease showed remarkable stability in anionic (5 mM SDS) and ionic (1% Trion X-100 and 1% Tween 80) detergents. It retained 97±2% and 83.6±1.1% of its initial activity after 1 h preincubation in the presence of 1 % H(2)O(2) and 1 % sodium perborate, respectively. Furthermore, the purified enzyme showed excellent stability and compatibility with some commercial laundry detergents besides its stain removal capacity. Considering these promising properties, VSG-4 protease may find tremendous application in laundry detergent formulations.

show abstract

“…Microbial proteases represent one of the three largest groups of industrial enzymes, corresponding to approximately 60% of the total sale of industrial enzymes worldwide (Rai and Mukherjee, 2010).…”

Section: Troductionmentioning

confidence: 99%

Effect of pH and temperature on the production and activity of Schwanniomyces polymorphus extracellular proteases in fermentation medium

Alexander¹,

Cleiton²,

Dangely³

et al. 2015

Afr. J. Microbiol. Res.

View full text Add to dashboard Cite

show abstract

Statistical optimization of production, purification and industrial application of a laundry detergent and organic solvent-stable subtilisin-like serine protease (Alzwiprase) from Bacillus subtilis DM-04

Cited by 94 publications

References 29 publications

Characterization of a multifunctional feather-degrading Bacillus subtilis isolated from forest soil

Characterization of a multifunctional feather-degrading Bacillus subtilis isolated from forest soil

Purification and partial characterization of a detergent and oxidizing agent stable alkaline protease from a newly isolated Bacillus subtilis VSG-4 of tropical soil

Effect of pH and temperature on the production and activity of Schwanniomyces polymorphus extracellular proteases in fermentation medium

Contact Info

Product

Resources

About