Statistical optimization of process parameters for inulinase production from Tithonia weed by Arthrobacter mysorens strain no.1

Kamble, Prajakta P.; Kore, Maheshkumar Vishnu; Patil, Satish S.; Jadhav, Jyoti P.; Attar, Yasmin C.

doi:10.1016/j.mimet.2018.04.019

Cited by 16 publications

(12 citation statements)

References 28 publications

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“…Different shapes of contour plots indicated different type of interactions between the factors. The elliptical shape showed that interactions between corresponding factors were significant, although the reciprocal interactions between the corresponding factors are insignificant as represented by a circular contour plot (Kamble et al, 2018). The quadratic equation and ANOVA showed that AC (PCA concentration and hydrolysis time) have strength interaction.…”

Section: Resultsmentioning

confidence: 99%

Optimization of extraction conditions and determination of purine content in marine fish during boiling

Ren

Dangfeng

et al. 2019

PeerJ

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Background Gout is the second most common metabolic disease affecting human health. The disease of gout is closely related to the level of uric acid, which is the end-product of human purine metabolism. Moreover, food is the main way of external ingestion of purine. Method A simple and time-saving method was developed to extract purines like adenine, hypoxanthine, guanine, and xanthine from marine fish by single factor design combined with Box–Behnken. The contents of these purines in the edible parts and internal organs of marine fish, as well as Scophthalmus maximus, were determined by high-performance liquid chromatography to investigate the relationship between the boiling process and purine content. Result The mixed-acid method was chosen for the extraction of purine bases and the extraction conditions were as follows: mixture acid 90.00% TFA/80.00% FA (v/v, 1:1); hydrolysis temperature 90.00 °C; time 10.00 min; liquid-to-solid ratio 30:1. The total purine content of the edible parts (eyes, dorsal muscles, abdominal muscles, and skin) was the highest in Scophthalmus maximus, followed by sphyraena, Sardinella, Trichiurus lepturus, Scomberomorus niphonius, Pleuronectiformes, Sea catfish, Anguillidae, and Rajiformes. Moreover, boiling significantly reduced the purine content in the marine fish because of the transfer of the purines to the cooking liquid during boiling. Scophthalmus maximus, Sphyraena, and Sardinella were regard as high-purine marine fish, which we should eat less. We also confirmed that boiling significantly transferred purine bases from fish to cooking liquid. Thus, boiling could reduce the purine content of fish, thereby reducing the risk of hyperuricemia and gout.

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Section: Resultsmentioning

confidence: 99%

Optimization of extraction conditions and determination of purine content in marine fish during boiling

Ren

Dangfeng

et al. 2019

PeerJ

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“…The R 2 values were evaluated as 0.9558 for the activity of the IHRP, 0.9747 for the protein binding yield with fitted model; those were in reasonably close with the Radj 2 of 0.9117 and 0.9493, respectively. The difference between R 2 and Radj 2 is <0.2 suggesting that sample range is adequately large [29,30].…”

Section: Immobilization Of Hrp On Tinps-coohmentioning

confidence: 95%

Optimization of the Immobilization Conditions of Horseradish Peroxidase on TiO2-COOH nanoparticles by Box-Behnken Design

Şahin

2019

Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi

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In this study, TiO2 nanoparticles were prepared and-COOH functionalized with 3-(3,4-dihydroxyphenyl) propionic acid. The characterization of nanoparticles was performed by FTIR, TEM, EDS and XRD. HRP was immobilized on those nanoparticles by EDC/NHS coupling reaction. The immobilization conditions of HRP including A: enzyme concentration (0.5-1.5 mg/mL), B: immobilization pH (4.0-8.0), C: immobilization temperature (4-50°C), D: immobilization time (1-20 h) were optimized by response surface methodology and Box-Behnken design. The optimized immobilization conditions were identified as 0.5 mg/mL HRP, at pH 5.5, 40 °C for 8 h for activity of immobilized HRP, 1.5 mg/mL HRP, at pH 4 and 18°C for 20 h for protein binding yield (%). At these optimum conditions, the experimental value for the activity of immobilized HRP was 80.39 U ± 1.06; protein binding yield was 94.25 ± 3.58%. Moreover, the optimum temperature and pH of free and immobilized enzyme were determined as 50°C and 4.0; 50°C and 3.5, respectively. The activity of the immobilized HRP sustained 52% of its initial activity after 10 days storage at 4°C. Furthermore, the immobilized HRP sustained 48% of its initial activity after 6 consecutive reactions.

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“…It also includes variety of statistical techniques used for experimental design and model erection that measures and scrutinizes the optimum conditions. Effective optimization of fungal, bacterial, and yeast inulinase production consuming diverse substrates such as Jerusalem artichoke, sugarcane bagasse, and molasses in submerged or solid-state cultivation was stated in the literature [27]. Diagrammatic depiction of microorganisms and optimized experimental variables is accessible in Figure 6 [13,16,[28][29][30][31][32][33].…”

Section: Factorial Designmentioning

confidence: 99%

Bioconversion of Weedy Waste into Sugary Wealth

Kamble¹,

Suryawanshi²,

Kore³

et al. 2020

Microorganisms

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Efforts put in overriding the inulin abundant invader nastiest category I weeds are infeasible that lead into its impermanent confiscation. Hence, their heedful exploitation is obligatory. These invasive weeds have ample amount of inulin, which serves as a renewable, cheap raw substrate for inulinase production. Therefore, they have enticed intention of many researchers toward exploring more idiosyncratic inulinase producing microbial strains that utilize invasive inulin-rich weeds as substrate for fructose liberation. Plenteous industrial applications of inulinases have marked it distinctly crucial in recent biotechnological epoch. This review thus elaborates the literature on infused footprints embedded by the substituted low calorie healthy sweetener in new advancing fields.

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Statistical optimization of process parameters for inulinase production from Tithonia weed by Arthrobacter mysorens strain no.1

Cited by 16 publications

References 28 publications

Optimization of extraction conditions and determination of purine content in marine fish during boiling

Optimization of extraction conditions and determination of purine content in marine fish during boiling

Optimization of the Immobilization Conditions of Horseradish Peroxidase on TiO2-COOH nanoparticles by Box-Behnken Design

Bioconversion of Weedy Waste into Sugary Wealth

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