Statistical optimization of physical process variables for bio-plastic (PHB) production by Alcaligenes sp.

Tripathi, Abhishek Dutt; Srivastava, S. K.; Singh, Ravi Pratap

doi:10.1016/j.biombioe.2013.02.017

Cited by 57 publications

(23 citation statements)

References 19 publications

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“…Using the method of factorial design and response surface analysis, it was possible to determine optimal operating conditions, which led to higher concentrations and productivity without any loss of yield. Shake flask cultivation studies depicted 7.48 g/L PHB content under optimized nutrient and physical variables, which is higher than the previous report under similar condition (Tripathi et al 2013). Optimized media obtained by response surface methodology consisted of fructose, 35 g/L; KH 2 PO 4 , 1.75 g/L; MgSO 4 Á7H 2 O 1.2 g/L, Citric acid 1.7 g/L, trace element 10 mL/L, initial pH = 7, agitation speed 175, and temperature 30°C.…”

Section: Resultscontrasting

confidence: 79%

“…A design of 16 experiments was formulated for seven factors using the software. Concentration range for the variables was decided on the basis of other reports for PHB production by C. necator (Tripathi et al 2013;Khanna and Srivastava 2005). The experiments were done in flasks containing 100 mL media at 200 rpm for 48 h in duplicate.…”

Section: Plackett-burman Design and Statistical Analysis For Optimizamentioning

confidence: 99%

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Statistical physical and nutrient optimization of bioplastic polyhydroxybutyrate production by Cupriavidus necator

Aramvash

Shahabi

Aghjeh

et al. 2015

Int. J. Environ. Sci. Technol.

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Polyhydroxyalkanoates are biodegradable polymer materials that accumulate in numerous bacteria. The polyhydroxybutyrate is the most common type of polyhydroxyalkanoates, which potentially serves as precursor for bioplastic production. The most extensively studied polyhydroxybutyrate producing bacteria is Cupriavidus necator due to its capability to accumulate large amounts of this biopolymer in simple culture medium. Accumulation of polyhydroxyalkanoates granules in the cytoplasm of C. necator significantly depended on pH, aeration, carbon sources, nitrogen sources, and minerals in the culture medium. In the present study, the effect of both nutritional and physical variables on polyhydroxybutyrate production was investigated in order to optimize these conditions. At first, on the basis of one-factor-at-a-time experiments, fructose and ammonium chloride were found to be the most suitable sources of carbon and nitrogen for biopolymer production. Then the most significant factors affecting granules accumulation were recognized as fructose, agitation speed, KH 2 PO 4 , and initial pH using the Plackett-Burman and central composite design. ANOVA analysis showed significant interaction between fructose and agitation speed. After optimization of the medium, compositions for polyhydroxybutyrate production were determined as follows: fructose 35 g/L, KH 2 PO 4 1.75 g/L, MgSO 4 Á7H 2 O 1.2 g/L, citric acid 1.7 g/L, trace element 10 mL/L, initial pH = 7, and agitation speed 175 rpm. Under this optimal culture conditions, the maximum yield of PHB was 7.48 g/L. The present strategies included in this study could be used for PHB production by this bacterium. These results are the highest values of PHB ever obtained from batch culture of C. necator reported so far.

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Section: Resultscontrasting

confidence: 79%

Section: Plackett-burman Design and Statistical Analysis For Optimizamentioning

confidence: 99%

Statistical physical and nutrient optimization of bioplastic polyhydroxybutyrate production by Cupriavidus necator

Aramvash

Shahabi

Aghjeh

et al. 2015

Int. J. Environ. Sci. Technol.

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“…62 In a similar report, an improved batch P(3HB) production of 8.8 g L -1 with a P(3HB) content of 80 % dcw was achieved under statistically optimized conditions. 34 Kinetics analysis and mathematical modelling for PHA process optimization One of the most essential fermentation requirements for ensuring high PHA accumulation in most PHA production processes is maintenance of appropriate concentrations of excess carbon, along with limited availability of nitrogen in the fermentation medium during cultivation. On the other hand, the concept of dual nutrient limitation involving both carbon and nitrogen for tailored synthesis of PHAs has also been studied.…”

Section: Process Optimization Using Design-of-experiments Methodologymentioning

confidence: 99%

“…However, quite interestingly, there have been only few reports on the utilization of pure or refined substrates (sugars) for P(3HB) production, [27][28][29][30] while different types of inexpensive carbon sources such as agro-industrial wastes including cane molasses, sugar beet juice, rice straw hydrolysate, grass biomass hydrolysate, plant oils e.g., coconut oil, have been largely investigated. [31][32][33][34][35][36][37][38] Alcaligenes and Bacillus sp. remain the microorganisms of choice for P(3HB) production (Table 1).…”

Section: Batch Fermentationmentioning

confidence: 99%

Strategies for Large-scale Production of Polyhydroxyalkanoates

Kaur

Roy

2015

Chem.Biochem.Eng.Q.

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Polyhydroxyalkanoates (PHAs) are biodegradable and biocompatible intracellular polyesters that are accumulated as energy and carbon reserves by bacterial species, under nutrient limiting conditions. Successful large-scale production of PHAs is dependent on three crucial factors, which include the cost of substrate, downstream processing cost, and process development. In this respect, design and implementation of bioprocess strategies for efficient PHA bioconversions enable high PHA concentrations, yields and productivities. Additionally, development of PHA fermentation processes using inexpensive substrates, such as agro-industrial wastes, facilitates further cost reduction, thus benefitting large-scale PHA production. Thus, the aim of this review is to highlight various bioprocess strategies for high production of PHAs and their novel copolymers in relatively large quantities. This review also discusses the application of kinetic analysis and mathematical modelling as important tools for process optimization and thus improvement of the overall process economics for large-scale production of PHAs.

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“…Generally, the PHB is produced by anaerobic fermentation of sucrose from sugarcane by natural microorganisms and purified by natural solvent 9,10 . In addition, PHB also can be produced under aerobic conditions 11 and other carbon sources, e.g, sugars such as glucose and xylose 12 , carbohydrates sources as sugarbeet juice 13 , agricultural residues 14 and even fatty acids 15 .…”

Section: Introductionmentioning

confidence: 99%

Polyhydroxybutyrate Composites with Random Mats of Sisal and Coconut Fibers

et al. 2016

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Biodegradable polymeric composites using natural fibers have been investigated aiming to mitigate environmental impacts. In this paper, polyhydroxybutyrate (PHB) composites obtained using random mats of sisal and coconut fibers by compression molding in a hydraulic press, and the fiber content varied between 10% and 15% relative to the weight of the polymer. Thermal analyses were performed such as Differential Scanning Calorimetry (DSC) and Thermogravimetric Analysis (TGA). Flexural and tensile tests were performed before and after conditioning in climate chamber with temperature and moisture. The results of thermal analysis show that the thermal stability of the materials remained, both PHB without fiber as for composites with natural fibers mats. The results of mechanical tests indicated that the PHB without fibers and composites showed similar flexural strength values, while the results of the tensile test PHB without fibers showed resistance to higher tensile composite.

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Statistical optimization of physical process variables for bio-plastic (PHB) production by Alcaligenes sp.

Cited by 57 publications

References 19 publications

Statistical physical and nutrient optimization of bioplastic polyhydroxybutyrate production by Cupriavidus necator

Statistical physical and nutrient optimization of bioplastic polyhydroxybutyrate production by Cupriavidus necator

Strategies for Large-scale Production of Polyhydroxyalkanoates

Polyhydroxybutyrate Composites with Random Mats of Sisal and Coconut Fibers

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