Statistical Optimization of mineral salt medium components for Achromobacter xylos GSR21 production using Central Composite Design (CCD)

Abstract

Background: Achromobacter xylos strain GSR21 plays a crucial role in bioremediation of fossil fuel contamination, biopharmaceutical, cosmetics, chemical, petroleum refining, petrochemical, food industries and tertiary oil recovery (MEOR). Aim: within the present paper, to reinforce the censorious medium constituents for the assembly of Achromobacter xylos strain GSR21 by using response surface quadratic models (RSQM). Materials and methods: Response surface method (RSM) was utilized to make your mind up the best degrees of cycle factors (agar powder, yeast concentrate, FeSO4.7H2O, and KH2PO4). CCD design of RSM was utilized to contemplate the four factors at five levels, and Achromobacter xylos strain GSR21 fixation was estimated as reaction. Results: Relapse coefficients were dictated by relapse examination, and therefore the model condition was settled. R2 regard for bio-surfactant (g/L) was attempted to be 0.88, showing that the model fitted well with the exploratory results. Affirmation of the mathematical model was driven by playing out the examination with the foreseen updated values, and bio-surfactant yield was found to be 9.88 g/L. Endorsement of the foreseen model was fitted 98.8% with the test outcomes coordinated under the perfect conditions. Conclusion: In light of the above outcomes agar powder and yeast separate was perceived as compelling fragments for Achromobacter xylos GSR21 creation.