Statistical optimization of medium components for the production of chitinase by Alcaligenes xylosoxydans

Vaidya, R. J.; Vyas, Pranav; Chhatpar, H. S.

doi:10.1016/s0141-0229(03)00100-5

Cited by 70 publications

(36 citation statements)

References 15 publications

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“…CaCl 2 (0.12mg/ml), NaCl (1.2 mg/ml), KH 2 PO 4 (0.06 mg/ml), KBr (0.02 mg/ml), MgSO 4 7H 2 O (0.02 mg/ml), NH 4 NO 3 (0.05 mg/ml) and KNO 3 (0.02 mg/ml) were found to activate chitinase I, II and IIII [15]. Therefore the same salt concentrations were selected for the investigation of the thermalstability of these enzymes.…”

Section: Effect Of Salts On Thermalstabilitymentioning

confidence: 99%

“…Chitinases therefore play vital role in agricultural industries and medical fields, furthermore, chitinases also play vital roles in sea food industry for crustacean chitinous waste degradation. To accelerate identification of optimal chitinase formulations to function broadly in a range of environments for the biocontrol of phytopathogenic fungi, for cytochemical localization of chitin/chitosan using chitinase-chitosanase-gold complex, for fungal protoplast technology, for preparation of chitooligosaccharides and for degradation of chitinous waste [15], we have gained a fundamental understanding of behaviour of chitinase molecules in various conditions. For this, the study of activity and stability of chitinase enzymes in various conditions is necessary because, for example, the success of the biocontrol agent depends on geological and soil conditions where it is applied [12].…”

Section: Introductionmentioning

confidence: 99%

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Thermalstabilization of chitinolytic enzymes of Pantoea dispersa

Gohel

Naseby

2007

Biochemical Engineering Journal

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Section: Effect Of Salts On Thermalstabilitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Thermalstabilization of chitinolytic enzymes of Pantoea dispersa

Gohel

Naseby

2007

Biochemical Engineering Journal

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“…A atividade de quitinase foi determinada usando quitina coloidal (RÉISSIG;STROMINGER;LELOIR, 1955;SANDHU;WADHWA;BAGGA, 1989), de acordo com a metodologia otimizada por Yamaguchi (2003). A quitina coloidal foi preparada como descrito por Fleuri (2005).…”

Section: Determinação Da Atividade De Quitinaseunclassified

“…Segundo os autores, o método foi eficiente, somente 20 experimentos foram necessários para determinar as condições ótimas, o modelo foi satisfatório e o coeficiente de determinação foi de 0,94. Vaidya et al (2003) otimizaram os componentes do meio de cultivo para a produção de quitinase pela bactéria Alcaligenes xylosoxydans IMI 385022. Os componentes considerados relevantes para a produção de quitinase através do planejamento de Plackett-Burman foram: Tween 20, extrato de levedura e quitina.…”

Section: Introductionunclassified

Estudo da influência de diferentes parâmetros na produção de enzimas líticas

Fleuri¹,

Sato²

2008

Ciênc. Tecnol. Aliment.

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A parede celular de leveduras é formada por três principais grupos de polissacarídeos: -glucana ( -1,3 glucana e -1,6 glucana), mananaproteínas e quitina. A -glucana é o composto majoritário da parede celular de leveduras, seguida pelas mananaproteínas e pela quitina. A camada externa da parede celular de leveduras é formada pelas mananaproteínas, enquanto a interna, pela glucana (ANDREWS; ASENJO, 1987a,b). Diferentemente da parede celular das leveduras, a parede celular de fungos é composta principalmente por quitina (SAHAI; MANOCHA, 1993).As -1,3 glucanases, proteases líticas e quitinases são capazes de hidrolisar componentes da parede celular de microrganismos e, por isso, apresentam atividade lítica sobre leveduras e fungos (SELITRENIKOFF, 2001).Modelos matemáticos têm sido cada vez mais utilizados para ajudar a explicar respostas de reações bioquímicas. A metodologia de superfície de resposta é freqüentemente usada para determinar uma resposta ótima entre uma faixa específica de condições experimentais. Em muitos casos, a interação de parâmetros que influenciam processos fermentativos pode ser avaliada com um número reduzido de ensaios através de um planejamento experimental (THÉODORE; PANDA, 1995). Em relação às enzimas líticas, a metodologia de superfície de resposta é freqüentemente utilizada para a otimização e verificação da influência dos componentes do meio de cultivo para a produção das enzimas, assim como para a otimização e a verificação da influência de parâmetros na produção enzimática. AbstractThe aim of this work was to study the effects of different parameters in the production of -1,3 glucanases, proteases and chitinases by Cellulosimicrobium celulans 191, in culture media A, B and C, respectively. The experimental designs employed were 2 3 factorial designs, and the factors studied were: initial pH, temperature and rotary shaker speed. The experimental results for the production of -1,3 glucanase in culture medium A showed maximum activity (0.64 U.mL -1 ) with an initial pH of 8.5 after 24 hours of fermentation at 33 °C and 200 rpm. The parameters pH, rotary shaker speed and the interaction of all the parameters presented statistical significance. The experimental results for protease production in culture medium B showed maximum activity (4.25 U.mL -1 ) with an initial pH of 6.5 after 30 hours of fermentation at 20 °C and 200 rpm. The pH was the only parameter that presented statistical significance. The experimental results for chitinase production in culture medium C showed maximum activity (7.06 U.mL -1 ) at an initial pH of 5.5 after 72 hours of fermentation at 25 °C and 200 rpm. All the parameters and their interactions presented statistical significance. The determination coefficients of the analysis of variance and F tests demonstrated that the linear regression models obtained were significant at a confidence level of 95%. ResumoO presente trabalho visou o estudo do efeito de diferentes variáveis na produção de -1,3 glucanases, proteases e de quitinases pela linhagem Cellulosimicr...

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“…Chitinase is a group of enzymes capable of catalyzing the hydrolysis of chitin directly to its monomer N-acetyl-D-glucosamine from variety of sources, such as bacteria, fungi, yeast and plants (Patil et al, 2000). Chitinases have been applied to a broad range: (1) cytochemical localization of chitin/chitosan, (2) fungal protoplast technology, (3) preparation of chitooligosaccharides and (4) as biocontrol agents of fungal pathogens and plant parasitic nematodes (Vaidya et al, 2003). For example, chitinases from microorganisms can degrade fungal cell walls and the nematode eggshells which composed of chitin (Goodrich and Winter, 2007;Bird and McClure, 1976;Ordentlich et al, 1988;Regev et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

Optimization of Medium Components for the Production of Antagonistic Lytic Enzymes Against Phytopathogenic Fungi and Their Biocontrol Potential

Lee¹,

Neung²,

Park³

et al. 2014

Korean Journal of Soil Science and Fertilizer

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In this paper, fractional factorial screening design (FFSD) and central composition design (CCD) were used to optimize the medium components for producing chitinase and gelatinase by Lysobacter capsici YS1215. Crab shell powder, nutrient broth and gelatin were proved to have significant effects on chitinase and gelatinase activity by FFSD first. An optimal medium was obtained by using a three factor CCD, which consisted of nutrient broth of 2.0 g L ). This value was 3.76 and 1.11 fold of the chitinase and gelatinase activity, respectively, compared to the lowest productive medium in the design matrix. In investigating potential of these enzymes partially purified from L. capsici YS1215 for biotechnological use, the crude enzymes was found to be inhibition against pathogenic fungal mycelia: Colletotrichum gleosporioides, Phytophthora capsici, and Rhizoctonia solani. In this study, we demonstrated the optimal medium for producing the chitinolytic and gelatinolytic enzymes by the strain YS1215 and the role of their enzymes that may be useful for further development of a biotechnological use and agricultural use for biological control of phytopathogenic fungi.

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Statistical optimization of medium components for the production of chitinase by Alcaligenes xylosoxydans

Cited by 70 publications

References 15 publications

Thermalstabilization of chitinolytic enzymes of Pantoea dispersa

Thermalstabilization of chitinolytic enzymes of Pantoea dispersa

Estudo da influência de diferentes parâmetros na produção de enzimas líticas

Optimization of Medium Components for the Production of Antagonistic Lytic Enzymes Against Phytopathogenic Fungi and Their Biocontrol Potential

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