Statistical optimization of lipase production from Sphingobacterium sp. strain S2 and evaluation of enzymatic depolymerization of Poly(lactic acid) at mesophilic temperature

Satti, Sadia Mehmood; Abbasi, Amina Mumtaz; Salahuddin,; Rana, Qurrat ul ain; Marsh, Terrance L.; Auras, Rafael; Hasan, Fariha; Badshah, Malik; Farman, Muhammad; Shah, Aamer Ali

doi:10.1016/j.polymdegradstab.2018.11.030

Cited by 16 publications

(7 citation statements)

References 42 publications

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“…[19][20][21] In turn, biotechnological processes using enzymatic biocatalysts have a high ecological profile, but they are less effective than chemical approaches. 22,23 The most interesting seem to be catalysts similar to those used in the ROP of cyclic esters. However, the studies of well-characterized catalytic systems for polyester degradation based on coordination metal compounds are limited, with the set including only a few examples.…”

Section: Introductionmentioning

confidence: 99%

Sodium complexes as precise tools for cutting polymer chains. Exploration of PLA degradation by unique cooperation of sodium centers

Nizioł

Jędrzkiewicz

Wiencierz

et al. 2023

Inorg. Chem. Front.

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Section: Introductionmentioning

confidence: 99%

Sodium complexes as precise tools for cutting polymer chains. Exploration of PLA degradation by unique cooperation of sodium centers

Nizioł

Jędrzkiewicz

Wiencierz

et al. 2023

Inorg. Chem. Front.

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“…Anthranilate phosphoribosyltransferase (EC 2.4.2.18) [97] and 5 -nucleotidase (EC 3.1.3.5), reported to have an impact on virulence and biofilm formation, were found in our Geobacil-lus sp. EC-3 [98]. Cyclic-di-AMP, well known for its role in the regulation of diverse cellular pathways, including potassium homeostasis, biofilm formation, and cell wall biosynthesis, was also detected in our strain [99].…”

Section: Genetic Determinants For Biofilm Formation and Regulationmentioning

confidence: 59%

“…In our previous studies we have reported the expression of PLA hydrolyzing lipase and esterase from our isolates P. aeruginosa strain S3 and Sphingobacterium sp. strain S2 [12,97,98]. Genome annotation of S2, S3 and EC-3 reveal the presence of numerous hydrolytic enzymes (Table 5).…”

Section: Enzymesmentioning

confidence: 99%

Genome Annotation of Poly(lactic acid) Degrading Pseudomonas aeruginosa, Sphingobacterium sp. and Geobacillus sp.

Satti

Castro‐Aguirre

Shah

et al. 2021

IJMS

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Pseudomonas aeruginosa and Sphingobacterium sp. are well known for their ability to decontaminate many environmental pollutants while Geobacillus sp. have been exploited for their thermostable enzymes. This study reports the annotation of genomes of P. aeruginosa S3, Sphingobacterium S2 and Geobacillus EC-3 that were isolated from compost, based on their ability to degrade poly(lactic acid), PLA. Draft genomes of the strains were assembled from Illumina reads, annotated and viewed with the aim of gaining insight into the genetic elements involved in degradation of PLA. The draft genome of Sphinogobacterium strain S2 (435 contigs) was estimated at 5,604,691 bp and the draft genome of P. aeruginosa strain S3 (303 contigs) was estimated at 6,631,638 bp. The draft genome of the thermophile Geobacillus strain EC-3 (111 contigs) was estimated at 3,397,712 bp. A total of 5385 (60% with annotation), 6437 (80% with annotation) and 3790 (74% with annotation) protein-coding genes were predicted for strains S2, S3 and EC-3, respectively. Catabolic genes for the biodegradation of xenobiotics, aromatic compounds and lactic acid as well as the genes attributable to the establishment and regulation of biofilm were identified in all three draft genomes. Our results reveal essential genetic elements that facilitate PLA metabolism at mesophilic and thermophilic temperatures in these three isolates.

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“…Zhao J et al found that the lipase isolated from SJXYZ strain was able to maintain viability in the pH range of 5.0-9.0. Gao K et al found that Streptomyces violaceus OUC-lipase showed optimal activity at pH 9.0 [23][24][25].…”

Section: Discussionmentioning

confidence: 99%

Prokaryotic Expression and Enzymatic Properties of Lipase from Schizochytrium Pombe

Liu¹,

Li²

2023

J Food Tech Nutri Sci

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Based on the codon preference of Escherichia coli, the gene of lipase from Schizochytrium pombe was optimized and the codon adaptation index was 0.8 and the gene was expressed in Escherichia coli as a His-tag fusion protein using pET-30a as the expression vector. The recombinant strain with OD600 about 0.6 was induced with 1 mmol/L isopropyl-β-D-thiogalactoside at 30 °C for 12 h. The purified recombinant lipase STGL3 had a molecular mass of 32 kDa, and the purified lipase had a specific enzyme activity of 2.29 μmol/(min•mg), which was 2.3 times higher than before, and a protein content of 650.00 mg/L. The recombinant STGL3 showed the highest hydrolysis activity at 40 °C and pH 7.5, respectively. The effect of different metal ion concentrations on enzyme activity varied, lipase STGL3 was stable in the presence of Ca2+, while Mg2+, Mn2+, Zn2+, Fe2+ and EDTA had an inhibitory effects on lipase activity. Different types of surfactants and organic solvents inhibited the enzymatic activity of recombinant lipase to different extents. The recombinant lipases showed a preference towards pNP-esters with medium and longer acyl-chains. (C12, C14 and C16) and the recombinant lipases showed relatively weak lipase activity towards the shorter carbon chains. The kinetic constants of lipase were 0.29 mmol/L for Km, 2.28 mmol/(L•min-1 ) for Vmax, and 6.19 S-1 for Kcat. The present study not only achieved the expression and characterization of enzymatic properties of lipase from Leptosphaeria japonica, but also provided some basis for the study of enhancing the quality and quality of algal oil by means of genetic engineering, and further exploring the potential industrial applications of Leptosphaeria japonica lipase.

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Statistical optimization of lipase production from Sphingobacterium sp. strain S2 and evaluation of enzymatic depolymerization of Poly(lactic acid) at mesophilic temperature

Cited by 16 publications

References 42 publications

Sodium complexes as precise tools for cutting polymer chains. Exploration of PLA degradation by unique cooperation of sodium centers

Sodium complexes as precise tools for cutting polymer chains. Exploration of PLA degradation by unique cooperation of sodium centers

Genome Annotation of Poly(lactic acid) Degrading Pseudomonas aeruginosa, Sphingobacterium sp. and Geobacillus sp.

Prokaryotic Expression and Enzymatic Properties of Lipase from Schizochytrium Pombe

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