Statistical optimization of culture medium for neutral protease production by Aspergillus oryzae. Comparative study between solid and submerged fermentations on tomato pomace

Belmessikh, Aicha; Boukhalfa, Hayet; Mechakra-Maza, Aicha; Gheribi-Aoulmi, Z; Amrane, Abdeltif

doi:10.1016/j.jtice.2012.12.011

Cited by 51 publications

(34 citation statements)

References 46 publications

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“…These positive effects of the two protein-rich complexes on growth and protease synthesis by Bacillus cells match previous findings (Kaur et al, 2001;Patel et al, 2005;Anbu, 2013). Though the presence of casein enhances protease production in some Bacillus strains (Belmessikh et al, 2013), it is likely that casein is not among the best protease inducers for the experimental Bacillus strain. Similarly, slight negative effects of casein on enzyme production by other related bacterial strains have been formerly reported (Nilegaonkar et al, 2007;Daroit et al, 2011).…”

Section: Discussionsupporting

confidence: 88%

Isolation and characterization of a haloalkaliphilic protease producer bacterium from Wadi Natrun in Egypt

Lotfy¹,

Abd-El-Karim²,

El-Sharouny³

et al. 2017

Afr. J. Biotechnol.

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A Gram-positive, rod-shaped, spore-forming haloalkaliphilic bacterium designated as NA7 was isolated from the surface of a Helianthemum nummularium root sample obtained from Wadi Natrun in Egypt. Sequence analysis of the 16S rRNA gene revealed a Bacillus haloalkaliphilius strain as the closest match with 99% identity. In a shake flask culture containing 10% NaCl, adjusted to pH 10 and incubated at 37°C, the isolated strain produced thermostable extracellular alkaline protease with relatively stable maximum activity records (0.610-0.625 TU) within a relatively long stationary phase that exceeded 60 h. A 2-level fractional factorial design (Plackett-Burman) was then applied to screen for nutritional and cultivation factors regulating protease production by the isolate and to appraise their effects. Calculated statistical parameters revealed that NaCl and MgSO 4 are the most significant independent variables affecting alkaline protease production by NA7 and suggested a near-optimum culture condition. Verification of this predicted condition resulted in an alkaline protease specific activity record of 509 TU/mg protein with a 1.27 fold increase when compared to the basal medium culture.

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Section: Discussionsupporting

confidence: 88%

Isolation and characterization of a haloalkaliphilic protease producer bacterium from Wadi Natrun in Egypt

Lotfy¹,

Abd-El-Karim²,

El-Sharouny³

et al. 2017

Afr. J. Biotechnol.

View full text Add to dashboard Cite

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“…The production of enzymes is slow at the start of fermentation and then accelerates until it reaches a maximum. From there the high concentration of products inhibits enzyme activity through negative feedback (GOMES et al, 2000;CAMASSOLA et al, 2004;OMEMU et al, 2005).…”

Section: Resultsmentioning

confidence: 99%

“…also produced enzymes with higher activity at pH 5.0 and 6.0 (GOMES et al, 2000). Total cellulase from P. echinulatum, however, had higher activity at pH 4.5 (CAMASSOLA et al, 2004).…”

Section: Inmentioning

confidence: 95%

“…These costs may be reduced through the use of plant biomass as a source of microorganism culture. As such, the use of biomass as the raw material for the production of compounds with industrial applications through biological, thermal and/or chemical processes are becoming more popular with the advancements in technology (GALEMBECK et al, 2009;GHORAI et al, 2009;BELMESSIKH et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

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Production and Characterization of Cellulolytic Enzymes by Aspergillus Niger and Rhizopus Sp. By Solid State Fermentation of Prickly Pear

et al. 2016

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-Prickly palm cactus husk was used as a solid-state fermentation support substrate for the production of cellulolytic enzymes using Aspergillus niger and Rhizopus sp. A Box-Behnken design was used to evaluate the effects of water activity, fermentation time and temperature on endoglucanase and total cellulase production. Response Surface Methodology showed that optimum conditions for endoglucanase production were achieved at after 70.35 h of fermentation at 29.56°C and a water activity of 0.875 for Aspergillus niger and after 68.12 h at 30.41°C for Rhizopus sp. Optimum conditions for total cellulase production were achieved after 74.27 h of fermentation at 31.22°C for Aspergillus niger and after 72.48 h and 27.86°C for Rhizopus sp. Water activity had a significant effect on Aspergillus niger endoglucanase production only. In industrial applications, enzymatic characterization is important for optimizing variables such as temperature and pH. In this study we showed that endoglucanase and total cellulase had a high level of thermostability and pH stability in all the enzymatic extracts. Enzymatic deactivation kinetic experiments indicated that the enzymes remained active after the freezing of the crude extract. Based on the results, bioconversion of cactus is an excellent alternative for the production of thermostable enzymes.Keywords: Filamentous fungi. Response surface methodology. Bioprocesses. Semi-arid region. Enzymatic characterization. PRODUÇÃO E CARACTERIZAÇÃO DE ENZIMAS CELULOLÍTICAS POR ASPERGILLUS

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“…Meanwhile, Belmessikh et al [26] reported that protease activity was nine times higher in SSF (21,309 U/g) compared to SmF (2343.5 U/g) using A. oryzae on tomato pomace.…”

Section: Proteasementioning

confidence: 99%

Multi-enzymes Production Studies in Single Tray Solid State Fermentation with Opened and Closed System

Manan¹,

Webb²

2016

JLS

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Abstract:The robustness of A. awamori and A. oryzae as enzyme producers is exploited in fungal fermentation on agricultural solid waste. High-level production of extracellular glucoamylase, protease, cellulase and xylanase has been achieved. Three different types of 'waste' solids (wheat bran, soybean hulls and rapeseed meal) have been used in studies of solid state fermentation (SSF). The enzymes could be produced in significant levels by continuously supplying oxygen (O 2 ) through the tray system known as "closed" and "opened" tray systems. A perforated tray system was developed in this study that permits direct access to O 2 . Testing the tray system with different perforated mesh aperture sizes in this study did not yield different results in growth performance of A. awamori and A. oryzae. A. awamori and A. oryzae can be very versatile in producing various enzymes with different substrates with different starch, protein, hemicellulose and cellulose contents. These studies indicate that A. awamori is more suitable for the efficient production of multiple enzymes in the closed system including xylanase and cellulase, while the production of glucoamylase and protease is superior in the opened system. A. oryzae is more suitable for the efficient production of protease and cellulase in the closed system, while the production of protease is more favourable the opened system. A. awamori efficiently consumed starch in wheat bran medium and produced very high glucoamylase activity, and after that, the fungus efficiently produced other enzymes to degrade other complex nutrients such as protein, hemicellulose and cellulose. Meanwhile, A. oryzae efficiently consumed protein in rapeseed meal and produced very high protease activity. The ability of both filamentous fungi, to convert biomass through SSF bioconversion will have a great impact on food and agro-industry in every aspect of life from food and medicine to fuel.

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Statistical optimization of culture medium for neutral protease production by Aspergillus oryzae. Comparative study between solid and submerged fermentations on tomato pomace

Cited by 51 publications

References 46 publications

Isolation and characterization of a haloalkaliphilic protease producer bacterium from Wadi Natrun in Egypt

Isolation and characterization of a haloalkaliphilic protease producer bacterium from Wadi Natrun in Egypt

Production and Characterization of Cellulolytic Enzymes by Aspergillus Niger and Rhizopus Sp. By Solid State Fermentation of Prickly Pear

Multi-enzymes Production Studies in Single Tray Solid State Fermentation with Opened and Closed System

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