Statistical optimization of culture media for growth and lipid production of Botryococcus braunii LB572

Tran, Hai-Linh; Kwon, Ji-Sue; Kim, Z-Hun; Oh, You-Kwan; Lee, Choul‐Gyun

doi:10.1007/s12257-009-0127-7

Cited by 54 publications

(28 citation statements)

References 14 publications

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“…Phosphorus -usually in the form of phosphate -at concentrations up to 444 mg/L increased the biomass concentration and reduced the lipid content 7.3 fold and 1.7 fold, respectively [64]. Lipid productivity also increased 1.2 fold by enhancing the phosphate concentration from 40 mg/L to 83 mg/L [56]. However, it should be pointed out that phosphate was not a limiting factor to biomass growth [17] due to the B. braunii's ability to accumulate large amounts of surplus phosphorus [68].…”

Section: Growth Environmentmentioning

confidence: 98%

“…Carbon, nitrogen, phosphorus, sulfur, and iron are essential nutrients both for biomass productivity and for the quantity and quality of the synthesized lipids. To reach this target, many synthetic media have been investigated [20,23,24,28,29,39,[55][56][57][58][59][60], but only the modified Chu13 medium [29,30,61] has been shown to be optimal. However, commercial biodiesel production using this synthetic medium is mostly discouraged, as the high consumption rates of fossilbased fertilizers as the source of inorganic nutrients make it costly and unsustainable.…”

Section: Growth Environmentmentioning

confidence: 99%

“…Phosphorus and sulfur concentrations also have major impacts on both the growth and lipid productivity of B. braunii (LB 572), as demonstrated by statistical optimization of culture media [56]. Phosphorus -usually in the form of phosphate -at concentrations up to 444 mg/L increased the biomass concentration and reduced the lipid content 7.3 fold and 1.7 fold, respectively [64].…”

Section: Growth Environmentmentioning

confidence: 99%

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Botryococcus braunii for biodiesel production

Tasić

Pinto

Klein

et al. 2016

Renewable and Sustainable Energy Reviews

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Section: Growth Environmentmentioning

confidence: 98%

Section: Growth Environmentmentioning

confidence: 99%

Section: Growth Environmentmentioning

confidence: 99%

See 1 more Smart Citation

Botryococcus braunii for biodiesel production

Tasić

Pinto

Klein

et al. 2016

Renewable and Sustainable Energy Reviews

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“…Controlled conditions at 60 µmol photons/m 2 .s along with other parameters led to a biomass productivity of 40 mg/L.d (Lv et al, 2010). Optimization of media in terms of major components such as metal elements (Mandalam and Palsson, 1998), and specifically iron , potassium, and magnesium (Tran et al, 2010) increased lipid content to 56.6% and lipid and biomass productivities to 190 mg/L.d and 304 mg/L.d; respectively. In a recent study, medium optimization resulted in high lipid content of 59.6% and lipid productivity of 74 mg/L.d obtained under 750 mg/L nitrogen content and zero phosphorous content while iron was supplemented as well (Singh et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Improved lipid and biomass productivities in Chlorella vulgaris by differing the inoculation medium from the production medium

Hamedi

Mahdavi

Gheshlaghi

2016

BRJ

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Please cite this article as: Hamedi S., Mahdavi M.A., Gheshlaghi R. Improved lipid and biomass productivities in Chlorella vulgaris by differing inoculation medium from production medium. Biofuel Research Journal 10 (2016) HIGHLIGHTSUsing different media for inoculums preparation and microalgae production improved lipid and biomass productivities.When SH4 was selected for inoculation medium and N8 was selected for production medium, 130% increase in biomass productivity and 40% increase in lipid productivity was observed. Specific growth rate improved by differing inoculums preparation medium from production medium and changed from 0.0040/h to 0.0122/h. Improvement of biomass and lipid productivities is now one of the main concerns in commercialization of microalgae cultivation as a feedstock for algal biofuel production. Conventional photoautotrophic processes using well-studied and rich in oil strain of Chlorella vulgaris are not able to meet such demands. A new strategy of inoculating algae production medium with cells grown in a different medium from the production medium was proposed herein. More specifically, when SH4 was used as production medium and N8 was used as inoculation medium, biomass and lipid productivities increased by 2.33 folds and 1.44 folds, respectively, compared with when the production and inoculation media were the same, such as SH4. The findings of the present investigation showed that this cultivation scheme resulted in 52% increase in cell number and 54% increase in dry weight leading to improved productivities. Although by even considering this improvement, photoautotrophic cultivation of algae can hardly compete with the heterotrophic cultivation, the high cost of hydrocarbon supply required in large-scale heterotrophic processes marks the technique proposed in the present study as a promising approach for commercialization of algal biofuel production. ARTICLE INFO ABSTRACT © 2016 BRTeam. All rights reserved.Journal homepage: www.biofueljournal.com Hamedi et al. / Biofuel Research Journal 10 (2016) 410-416 Please cite this article as: Hamedi S., Mahdavi M.A., Gheshlaghi R. Improved lipid and biomass productivities in Chlorella vulgaris by differing inoculation medium from production medium.

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“…At present, the racemic Ketoprofen was widely used in clinical. Therefore, it has important research value and application value to effective chiral resolution of racemic Ketoprofen split for SKetoprofen [2].…”

Section: Introductionmentioning

confidence: 99%

Optimization of Lipase-Catalyzed Asymmetric Synthesis of Ketoprofen in Non-Aqueous Media by Response Surface Methodology

Liu

Yin

2016

2016 Eighth International Conference on Measuring Technology and Mechatronics Automation (ICMTMA)

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Lipase-catalyzed asymmetric synthesis of ketoprofen was developed by response surface methodology under Box-Behenkon experimental designs. The optimal reaction system was Novozym 435 from Candida Antarctica, 1 -propanol as acyl donors, isooctane as reaction medium, and water content of 0.5%. Optimization of reaction temperature, substrate molar ratio (acyl donors: ketoprofen) and enzyme concentration was carried out response surface methodology under Box-Behenkon experimental designs. Optimal conditions were: temperature 36.62 °C, substrate molar ratio 3.5:1, and enzyme concentration 4.91 g/L. the conversion was 46.68%, eep was 93.35%, and E was 73.8 at optimal conditions. Reaction progress at optimum synthesis conditions showed that very satisfactory conversion (64.74%) and production concentration (20.13 g/L) could be achieved in short time (6 h).

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Statistical optimization of culture media for growth and lipid production of Botryococcus braunii LB572

Cited by 54 publications

References 14 publications

Botryococcus braunii for biodiesel production

Botryococcus braunii for biodiesel production

Improved lipid and biomass productivities in Chlorella vulgaris by differing the inoculation medium from the production medium

Optimization of Lipase-Catalyzed Asymmetric Synthesis of Ketoprofen in Non-Aqueous Media by Response Surface Methodology

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