Statistical optimization of culture conditions for 1,3-propanediol by Klebsiella pneumoniae AC 15 via central composite design

Zheng, Zongming; Hu, Qiulong; Hao, Jian; Xu, Feng; Guo, Ni-Ni; Sun, Yuankui; Liu, Dehua

doi:10.1016/j.biortech.2007.02.038

Cited by 74 publications

(39 citation statements)

References 15 publications

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“…The kinetic model for substrate and energy consumption of microbial growth under substrate-sufficient conditions and the mathematical model of bioconversion of glycerol to 1,3-PD by K. pneumoniae were created (Sun et al, 2008;Zeng and Deckwer, 1995). However, it appears that possible intrinsic reason(s) should be sought in the intracellular metabolic flux, enzyme activities profile, internal redox state analysis, etc.…”

Section: Introductionmentioning

confidence: 99%

Physiologic mechanisms of sequential products synthesis in 1,3‐propanediol fed‐batch fermentation by Klebsiella pneumoniae

Zheng

Liu

et al. 2008

Biotech & Bioengineering

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The glycerol fed-batch fermentation by Klebsiella pneumoniae CGMCC 1.6366 exhibited the sequential synthesis of products, including acetate, 1,3-propanediol (1,3-PD), 2,3-butanediol, ethanol, succinate, and lactate. The dominant flux distribution was shifted from acetate formation to 1,3-PD formation in early- exponential growth phase and then to lactate synthesis in late-exponential growth phase. The underlying physiological mechanism of the above observations has been investigated via the related enzymes, nucleotide, and intermediary metabolites analysis. The carbon flow shift is dictated by the intrinsic physiological state and enzymatic activity regulation. Especially, the internal redox state could serve as a rate-controlling factor for 1,3-PD production. The q(1,3-PD) formation was the combined outcomes of regulations of glycerol dehydratase activity and internal redox balancing. The q(ethanol)/q(acetate) ratios demonstrated the flexible adaptation mechanism of K. pneumoniae preferring ATP generation in early-exponential growth phase. A low PEP to pyruvate ratio corresponded LDH activity increase, leading to lactate accumulation in stationary phase.

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Section: Introductionmentioning

confidence: 99%

Physiologic mechanisms of sequential products synthesis in 1,3‐propanediol fed‐batch fermentation by Klebsiella pneumoniae

Zheng

Liu

et al. 2008

Biotech & Bioengineering

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“…The 3-hydroxypropionaldehyde accumulation during fermentation has been described in anaerobic fermentation by K. pneumoniae, and when the initial glycerol concentration was superior to 430 mM, a high concentration of 3-HPA inhibited cell growth and production of 1,3-propanediol [33,34]. Zheng et al [35] had demonstrated a productivity of 1.81 g/l.h from 50 g/l of glycerol, controlling pH, aeration rate and stirring in the bioreactor at 6,48, 0.6 VVM and 318 rpm, respectively, and the yield and productivity of 71.03 g/l, 2.37 g/l.h respectively and 0.6425 mol/mol (1,3-PDO/glycerol) at a fermentation of 30 h with the initial glycerol, rate of stirring, air aeration and pH of 50 g/l, 320 rpm, 0.6 VVM and 6.5 by fed-batch fermentation maintaining glycerol to 20-30 g/l. To optimize 1,3-propanediol synthesis, two stage processes are ideal, in which cells can be cultured first in a rich medium, and 1,3-propanediol production may be induced by addition of glycerol on the stationary phase.…”

Section: Batch Fermentation Of K Pneumoniae Glc29mentioning

confidence: 99%

Experimental Design For 1,3-Propanediol Biosynthesis by K. Pneumoniae GLC29 Using Glycerol

Neto¹,

Silva²,

Coelho³

et al. 2017

JABB

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Biodiesel glycerol can be used for the production of 1,3-propanediol by bacteria. In this study on the production of 1,3-propanediol by Klebsiella pneumoniae GLC29 was possible to optimize media culture the production of 1,3-propanediol using statistical experimental design techniques and response surface methodology, where 11 variables were tested and only 5 were found significant (glycerol, yeast extract, ammonium sulfate, vitamin B12 and fumarate). Subsequently, production of 1,3-propanediol was verified in 0,75 liter reactors, both in batch and exponential fedbatch. It was possible to achieve 23.6 g/l of 1,3-propanediol in batch cultures using pure glycerol, 27 g/l of 1,3-propanediol in batch cultures using biodiesel glycerol, and 29.9 g/l of 1,3-propanediol in exponential fed-batch using biodiesel glycerol.

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“…The molar yield of 1,3-PD to glycerol of 0.47 mol•mol −1 was obtained [7]. Another study on bioconversion of raw glycerol into 1,3-PD by K. pneumoniae showed that the molar yield of 1,3-PD to glycerol of 0.41 mol•mol −1 [23]. In this study, the strain ZH-1 had a higher molar conversion rate than some strains of the previous study, whereas compared with the study of Yang et al…”

Section: Optimum Of Ph Value For K Pneumoniae Zh-1mentioning

confidence: 99%

Isolation and Identification of Alkali-Resistant 1,3-Propanediol Producing Strain

Zhao¹,

Wen²,

Rong³

et al. 2016

AiM

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Abstract1,3-Propanediol is a promising renewable resource produced by microbial production. It is mainly used in many synthetic reactions, particularly applied to the polymer synthesis and cosmetics industry. We described here the isolation of strain ZH-1, which has the ability of high production with 1,3-propanediol, from Fenhe River in China. It was classified as a member of K. pneumoniae after the study of phenotypic, physiological, biochemical and phylogenetic (16S rDNA). The initial glycerol concentration, fermentation time and pH value of strain ZH-1 were determined to be 50 g•L −1 , 36 h and 8.0. Under these conditions, the practical yield of 1,3-PD was 18.53 g•L −1 and a molar yield (mol 1,3-PD mol Glycerol −1 ) of 1,3-propanediol to glycerol of 0.497. In addition, we found that for the strain ZH-1, the optimum grown pH was 9.0, so we can determine that it is a new member of alkali-resistant strains.

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Statistical optimization of culture conditions for 1,3-propanediol by Klebsiella pneumoniae AC 15 via central composite design

Cited by 74 publications

References 15 publications

Physiologic mechanisms of sequential products synthesis in 1,3‐propanediol fed‐batch fermentation by Klebsiella pneumoniae

Physiologic mechanisms of sequential products synthesis in 1,3‐propanediol fed‐batch fermentation by Klebsiella pneumoniae

Experimental Design For 1,3-Propanediol Biosynthesis by K. Pneumoniae GLC29 Using Glycerol

Isolation and Identification of Alkali-Resistant 1,3-Propanediol Producing Strain

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