Statistical optimization of critical medium components for lipase production from Yarrowia lipolytica (MTCC 35)

Kishan, G.; Gopalakannan, P.; Muthukumaran, C.; Muthukumaresan, K. Thirumalai; Kumar, M. Dharmendira; Tamilarasan, K.

doi:10.1016/j.jgeb.2013.06.001

Cited by 16 publications

(10 citation statements)

References 17 publications

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“…Significance of each variable was determined using Student t test. These finding illustrates the order of significance of the variables affecting the 2,3-BDO production as: yeast extract > KH 2 PO 4 > K 2 HPO 4 > MnSO 4 > KCl [20]. Different media composition resulted in significant variation in 2,3-BDO production ranging from 2.71 to 3.82 g/L (Supplementary Table 1 Fig.…”

Section: Resultsmentioning

confidence: 70%

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Efficient production of (R,R)-2,3-butanediol from cellulosic hydrolysate using Paenibacillus polymyxa ICGEB2008

Adlakha¹,

Yazdani²

2014

J Ind Microbiol Biotechnol

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We report here the production of pure (R,R)-2,3-butanediol (2,3-BDO) isomer by the non-pathogenic Paenibacillus polymyxa ICGEB2008 using lignocellulosic hydrolysate as substrate. Experimental design based on Plackett-Burman resulted in identification of Mn and K as most crucial salt elements along with the yeast extract for 2,3-BDO production. Further experiments using Box-Behnken design indicated that both KCl and yeast extract together had major impact on 2,3-BDO production. Optimized medium resulted in 2,3-BDO production with 2.3-fold higher maximum volumetric productivity (2.01 g/L/h) and similar yield (0.33 g/g sugar) as compared to rich yeast extract-peptone-dextrose medium in the bioreactor studies. Considering that the balance substrate was channeled towards ethanol, carbon recovery was close to theoretical yield between the two solvents, i.e., 2,3-BDO and ethanol. Biomass hydrolysate and corn-steep liquor was used further to produce 2,3-BDO without impacting its yield. In addition, 2,3-BDO was also produced via simultaneous saccharification and fermentation, signifying robustness of the strain.

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Section: Resultsmentioning

confidence: 70%

“…ICGEB2008 was determined using Plackett-Burman screening design [20]. ICGEB2008 was determined using Plackett-Burman screening design [20].…”

Section: Resultsmentioning

confidence: 99%

Efficient production of (R,R)-2,3-butanediol from cellulosic hydrolysate using Paenibacillus polymyxa ICGEB2008

Adlakha¹,

Yazdani²

2014

J Ind Microbiol Biotechnol

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“…It was observed that A. niger C lipase activity (30.76 U.mL -1 ) obtained in this study were higher and/or similar to results reported in the literature: 4.52 U.mL -1 and 25.07 U.mL -1 [28] and 40.1 U.mL -1 [29], both employing the fungus Aspergillus sp. Using Trichoderma harzianum, Coradi et al [14] obtain 1.4 U.mL -1 while Kishan et al [8] found 9.40 U.mL -1 with Yarrowia lipolytica. These results indicate that the A. niger C strain produced the lipase enzyme at satisfactory levels when compared to levels already reported in the literature.…”

Section: Experimental Designmentioning

confidence: 99%

“…Lipase production is affected by the medium pH, temperature, medium composition, inoculation volume, aeration, agitation and many other factors. These factors have been studied in a number of microorganisms and several strategies have been used to model and to optimize the parameters of the fermentation process using statistical experimental designs [8].…”

Section: Introductionmentioning

confidence: 99%

Lipase Production by Aspergillus niger C by Submerged Fermentation

Lima

Gonçalves

Couri

et al. 2019

Braz. arch. biol. technol.

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This study aimed to evaluate the effects of variables on the process of lipases production by Aspergillus niger C by submerged fermentation (SmF). The production assays were performed in shake flasks for 72 hours at 150 rpm and 32°C. First, a fractional factorial design 2 5-1 (FFD) was carried out to evaluate the effect of the following process variables: sucrose, ammonium sulphate, soybean oil, yeast extract concentration and pH. After the selection of the variables that significantly influenced the lipase production, a central composite rotational design 2 2 (CCRD) was used, aiming to find the most favorable operational conditions. The selected assay condition (15.0 g.L-1 sucrose, 4.0 g.L-1 HIGHLIGHTS • Lipase was production by A. niger using sucrose and soybean oil as carbon source. • Experiment design was an efficient tool in improving A. niger lipase production. • Lipase production kinetics indicated good yield levels in a short fermentation time.

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“…The one‐factor‐at‐a‐time optimization design is simple, but fails to seek the optimum region because the interaction effects of variables are not considered. Response surface methodology (RSM) based on central composite design (CCD) is a collection of mathematical and statistical techniques for evaluating the relative significance of a multivariable system and determining the optimum conditions for various bioprocesses 31–35.…”

Section: Introductionmentioning

confidence: 99%

Fabrication of a Chitosan‐Coated Magnetic Nanobiocatalyst for Starch Hydrolysis

Jain

Sebatini

Sharmila³

et al. 2015

Chem Eng & Technol

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The preparation of chitosan-coated magnetic nanoparticles (MNPs) and covalent immobilization of a-amylase for starch hydrolysis was investigated. Surface morphology, chemical composition, and structural characteristics of the MNPs were analyzed by scanning electron microscopy, energy dispersion spectrometry, and X-ray diffractometry, respectively. Surface functional groups of MNPs, chitosancoated MNPs, and a-amylase-immobilized MNPs were characterized by Fourier transform infrared spectroscopy. Response surface methodology based on three levels was implemented to optimize three immobilization conditions and a regression model was developed. a-Amylase-immobilized MNPs provided better stability towards pH and temperature. The prepared thermostable nanobiocatalyst is well-suited for industrial processes involving starch hydrolysis.a-Amylase, chitosan, ferric chloride, ferrous sulfate, ammonium hydroxide, and solvents were purchased from Hi-media, www.cet-journal.com

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Statistical optimization of critical medium components for lipase production from Yarrowia lipolytica (MTCC 35)

Cited by 16 publications

References 17 publications

Efficient production of (R,R)-2,3-butanediol from cellulosic hydrolysate using Paenibacillus polymyxa ICGEB2008

Efficient production of (R,R)-2,3-butanediol from cellulosic hydrolysate using Paenibacillus polymyxa ICGEB2008

Lipase Production by Aspergillus niger C by Submerged Fermentation

Fabrication of a Chitosan‐Coated Magnetic Nanobiocatalyst for Starch Hydrolysis

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