Statistical Optimization of Alpha-Amylase Production with Immobilized Cells of Streptomyces erumpens MTCC 7317 in Luffa cylindrica L. Sponge Discs

Abstract

The purpose of this investigation was to study the effect of Streptomyces erumpens cells immobilized in various matrices, i.e., agar-agar, polyacrylamide, and luffa (Luffa cylindrica L.) sponge for production of alpha-amylase. Luffa sponge was found to be 21% and 51% more effective in enzyme yield than agar-agar and polyacrylamide, respectively. Response surface methodology was used to evaluate the effect of three main variables, i.e., incubation period, pH, and temperature on enzyme production with immobilized luffa cells. The experimental results showed that the optimum incubation period, pH, and temperature were 36h, 6.0, and 50 degrees C, respectively. The repeated batch fermentation of immobilized cells in shake flasks showed that S. erumpens cells were more or less equally physiologically active on the support even after three cycles of fermentation (3,830-3,575 units). The application of S. erumpens crude enzyme in liquefying cassava starch was studied. The maximum hydrolysis of cassava starch (85%) was obtained with the application of 4ml (15,200 units) of crude enzyme after 5 h of incubation.

Ethanol fermentation of mahula (Madhuca latifolia) flowers using free and immobilized bacteria Zymomonas mobilis MTCC 92

“…(Selvakumar et al 1994). A number of carrier materials have been used for entrapping microbial cells for bio-product production (Kar et al 2009). The most suitable carriers for cell immobilization is entrapment in calcium alginate as bead (Adinarayana et al 2005;Kar & Ray 2008) because this technique is simple and cost effective (Kourkoutas et al 2004).…”

Biosynthesis of industrial enzymes by free and immobilized Alicyclobacillus in different matrices and the use of ultrafiltration in the enzymes concentration

“…Kar et al, 41 studied the effect of different matrices in the Streptomyces erumpens immobilization for the amylase production, and verified that the loofa sponge presented excellent support due to the high value of aggregate biomass, being 21 and 51% more efficient than agar-agar and polyacrylamide also used, respectively.…”

Development of corn silk as a biocarrier for <i>Zymomonas mobilis</i> biofilms in ethanol production from rice straw

“…They remain productive for several months, are cost effective, less affected by inhibitory compounds, and facilitate product recovery (Behera et al, 2010;Chandel et al, 2007;Cheng et al, 2010;Rosche et al, 2009). Other conventional immobilization substrates, such as calcium polyurethane foam, alginate, gelatin and κ-carrageenan, can cause productivity loss due to the toxicity and poor stability of the carrier (Kar et al, 2009). Therefore, biofilm reactors have been developed to produce valuable compounds (Behera et al, 2010;Cheng et al, 2010).…”