Statistical optimization and characterization of a biocellulose produced by local Egyptian isolate Komagataeibacter hansenii AS.5

Saleh, Ahmed K.; Soliman, Nadia A.; Farrag, Ayman A.; Ibrahim, Maha M.; El-Shinnawy, N. A.; Abdel-Fattah, Yasser R.

doi:10.1016/j.ijbiomac.2019.12.103

Cited by 27 publications

(15 citation statements)

References 49 publications

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“…The NaOH treatment is considered able to eradicate the bacteria by cell lysis. Concentrations of NaOH higher than 5% are not recommended because this could change the crystalline structure of BC from cellulose I to cellulose II [ 53 , 54 ].…”

Section: Methodsmentioning

confidence: 99%

Antibacterial Activity of Bacterial Cellulose Loaded with Bacitracin and Amoxicillin: In Vitro Studies

et al. 2020

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The use of bacterial cellulose (BC) in skin wound treatment is very attractive due to its unique characteristics. These dressings’ wet environment is an important feature that ensures efficient healing. In order to enhance the antimicrobial performances, bacterial-cellulose dressings were loaded with amoxicillin and bacitracin as antibacterial agents. Infrared characterization and thermal analysis confirmed bacterial-cellulose binding to the drug. Hydration capacity showed good hydrophilicity, an efficient dressing’s property. The results confirmed the drugs’ presence in the bacterial-cellulose dressing’s structure as well as the antimicrobial efficiency against Staphylococcus aureus and Escherichia coli. The antimicrobial assessments were evaluated by contacting these dressings with the above-mentioned bacterial strains and evaluating the growth inhibition of these microorganisms.

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Section: Methodsmentioning

confidence: 99%

Antibacterial Activity of Bacterial Cellulose Loaded with Bacitracin and Amoxicillin: In Vitro Studies

et al. 2020

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“…The Plackett Burman design is an essential tool in screening the effects of process variables. Further, it is frequently used to enhance the product yield, reduces the process variability and number of experiments, process time and overall cost [21][22][23]. The Response surface methodology (RSM) with Box Behnken design is a widely used mathematical optimization tool due to simultaneous analysis of interactions of several parameters with the minimum of experimental trials.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

“…The XRD analysis was performed to distinguish the phases and to gain an overview of the morphology of the biomass and regularization of shear bands that form the structure of the polysaccharides [22]. The XRD pattern of shoot vines wastes were carried out at the CuKα, radiation (λ= 1.54056 A °).…”

Section: X-ray Diffraction (Xrd)mentioning

confidence: 99%

Characterization of cardinal vine shoot waste as new resource of lignocellulosic biomass and valorization into value-added chemical using Plackett–Burman and Box Behnken

Amine

Amrane

Aksil

et al. 2021

Biomass Conv. Bioref.

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The objective of this work was to valorize a waste from cardinal vine shoot into a hydro-lysate rich in reducing sugars. Plackett-Burman design was considered to identify the significant factors while a Box Behnken design was considered to optimize the extraction in the following experimental conditions: 100 °C, 750 rpm, trifluoracetic acid (CF3O2H) concentration (TFA) in the range (1 -10%), during 20 to 180 min and considering the following solid-liquid (S/V) ratios (1:1, 3:1, 5:1). The optimal result was 2.53% in sugars equivalent to a yield of 50.64% per g of dry matter. Shoot vine waste was characterized by Attenuated Total Reflection-Fourier Transform Infrared

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“…Gram-negative Komagataeibacter sp. that belong to a group of acetic acid bacteria are considered to be the model organism for BC production and have been extensively described in the literature 6 , 22 – 25 . By contrast, BC production from Gram-positive bacteria has been reported from only a few species such as Lactobacillus sp.…”

Section: Introductionmentioning

confidence: 99%

“…Optimizing the production, including the composition of the medium and parameters for cultivation conditions is critical for cost reduction. Various optimization techniques can be applied to enhance BC yields, including one variable at a time (OVAT) methods or statistical experimental designs (SED) 22 , 28 , 33 . The OVAT technique is a conventional optimization approach wherein variables are held constant and only one variable is altered.…”

Section: Introductionmentioning

confidence: 99%

Bioprocess development for bacterial cellulose biosynthesis by novel Lactiplantibacillus plantarum isolate along with characterization and antimicrobial assessment of fabricated membrane

Saleh

El‐Gendi

Soliman

et al. 2022

Sci Rep

Self Cite

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Bacterial cellulose (BC) is an ecofriendly biopolymer with diverse commercial applications. Its use is limited by the capacity of bacterial production strains and cost of the medium. Mining for novel organisms with well-optimized growth conditions will be important for the adoption of BC. In this study, a novel BC-producing strain was isolated from rotten fruit samples and identified as Lactiplantibacillus plantarum from 16S rRNA sequencing. Culture conditions were optimized for supporting maximal BC production using one variable at a time, Plackett–Burman design, and Box Behnken design approaches. Results indicated that a modified Yamanaka medium supported the highest BC yield (2.7 g/l), and that yeast extract, MgSO4, and pH were the most significant variables influencing BC production. After optimizing the levels of these variables through Box Behnken design, BC yield was increased to 4.51 g/l. The drug delivery capacity of the produced BC membrane was evaluated through fabrication with sodium alginate and gentamycin antibiotic at four different concentrations. All membranes (normal and fabricated) were characterized by scanning electron microscope, Fourier transform-infrared spectroscopy, X-ray diffraction, and mechanical properties. The antimicrobial activity of prepared composites was evaluated by using six human pathogens and revealed potent antibacterial activity against Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus, and Streptococcus mutans, with no detected activity against Pseudomonas aeruginosa and Candida albicans.

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Statistical optimization and characterization of a biocellulose produced by local Egyptian isolate Komagataeibacter hansenii AS.5

Cited by 27 publications

References 49 publications

Antibacterial Activity of Bacterial Cellulose Loaded with Bacitracin and Amoxicillin: In Vitro Studies

Antibacterial Activity of Bacterial Cellulose Loaded with Bacitracin and Amoxicillin: In Vitro Studies

Characterization of cardinal vine shoot waste as new resource of lignocellulosic biomass and valorization into value-added chemical using Plackett–Burman and Box Behnken

Bioprocess development for bacterial cellulose biosynthesis by novel Lactiplantibacillus plantarum isolate along with characterization and antimicrobial assessment of fabricated membrane

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