Statistical Improvement of Batch Culture with Immobilized <i>Pichia pastoris</i> Cells for rPOXA 1B Laccase Production

Gouzy-Olmos, Michelle; Cháves-Tequia, Luis M.; Rojas-Fajardo, María F.; Morales-Álvarez, Edwin D.; Rivera-Hoyos, Claudia M.; Poutou-Piñales, Raúl A.; González-Neira, Eliana María; Reyes-Montaño, Edgar Antonio; Cardozo-Bernal, Ángela M.; Gómez-Méndez, Luis D.; Pedroza-Rodríguez, Aura M.

doi:10.3844/ajbbsp.2018.88.107

Cited by 3 publications

(2 citation statements)

References 30 publications

(42 reference statements)

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“…Similar results obtained by Lassouane et al, 2019 by immobilizing crude laccase from Trametes pubescens MB89 on sodium alginate beads [222]. Although immobilization of enzymes is frequent, other investigations immobilized the wild-type or recombinant laccase-producing organisms [27,156].…”

Section: Stability Of Laccasessupporting

confidence: 79%

“…Biological ones are not effective for some of the coloured wastewater due to the toxicity of commercial dyes for the organisms used in the process [151]. However, it has led to the evaluation of treatments that do not require the direct use of the microorganism, such as adsorption by dead microbial biomass (biosorption), living immobilized biomass and the use of free or immobilized enzymes [8,27,156].…”

Section: Treatment Of Coloured Wastewatermentioning

confidence: 99%

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A Brief History of Colour, the Environmental Impact of Synthetic Dyes and Removal by Using Laccases

et al. 2021

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The history of colour is fascinating from a social and artistic viewpoint because it shows the way; use; and importance acquired. The use of colours date back to the Stone Age (the first news of cave paintings); colour has contributed to the social and symbolic development of civilizations. Colour has been associated with hierarchy; power and leadership in some of them. The advent of synthetic dyes has revolutionized the colour industry; and due to their low cost; their use has spread to different industrial sectors. Although the percentage of coloured wastewater discharged by the textile; food; pharmaceutical; cosmetic; and paper industries; among other productive areas; are unknown; the toxic effect and ecological implications of this discharged into water bodies are harmful. This review briefly shows the social and artistic history surrounding the discovery and use of natural and synthetic dyes. We summarise the environmental impact caused by the discharge of untreated or poorly treated coloured wastewater to water bodies; which has led to physical; chemical and biological treatments to reduce the colour units so as important physicochemical parameters. We also focus on laccase utility (EC 1.10.3.2), for discolouration enzymatic treatment of coloured wastewater, before its discharge into water bodies. Laccases (p-diphenol: oxidoreductase dioxide) are multicopper oxidoreductase enzymes widely distributed in plants, insects, bacteria, and fungi. Fungal laccases have employed for wastewater colour removal due to their high redox potential. This review includes an analysis of the stability of laccases, the factors that influence production at high scales to achieve discolouration of high volumes of contaminated wastewater, the biotechnological impact of laccases, and the degradation routes that some dyes may follow when using the laccase for colour removal

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Section: Stability Of Laccasessupporting

confidence: 79%

Section: Treatment Of Coloured Wastewatermentioning

confidence: 99%

A Brief History of Colour, the Environmental Impact of Synthetic Dyes and Removal by Using Laccases

et al. 2021

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Tertiary treatment (Chlorella sp.) of a mixed effluent from two secondary treatments (immobilized recombinant P. pastori and rPOXA 1B concentrate) of coloured laboratory wastewater (CLWW)

et al. 2020

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Pichia pastoris as a biological platform to produce recombinant proteins: relevance for development of biotechnological products in Colombia

Cabrera¹,

Rodríguez

2021

Rev. Fac. Cienc.

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The world market for compounds produced by biotechnological means is growing due to the search and implementation of cellular systems that allow the mass production of complex molecules with a specific biological activity. These range from drugs, to enzymes and proteins for diverse uses, such as academic research and the development of industrial processes. Pichia pastoris is a methylotrophic yeast that has been studied in recent decades for the expression and generation of recombinant proteins, because it has features that make it especially efficient, not only to host external DNA, but also to express it and, thus, produce a wide variety of molecules. In this study, the most important aspects related to the production of recombinant proteins are examined, by using P. pastoris as a model, from the most common expression strategy, to the aspects related to the cultivation at bioreactor scale and, by yielding high-value products. Some papers conducted, in Colombia, are also reviewed, as well as their approach and the current state of the expression system in the country's biotechnology and its barriers, by concluding that studies with P. pastoris are scarce and are mainly developed around a few academic centers.

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Statistical Improvement of Batch Culture with Immobilized Pichia pastoris Cells for rPOXA 1B Laccase Production

Cited by 3 publications

References 30 publications

A Brief History of Colour, the Environmental Impact of Synthetic Dyes and Removal by Using Laccases

A Brief History of Colour, the Environmental Impact of Synthetic Dyes and Removal by Using Laccases

Tertiary treatment (Chlorella sp.) of a mixed effluent from two secondary treatments (immobilized recombinant P. pastori and rPOXA 1B concentrate) of coloured laboratory wastewater (CLWW)

Pichia pastoris as a biological platform to produce recombinant proteins: relevance for development of biotechnological products in Colombia

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