Statistical design and optimization of single cell oil production from sugarcane bagasse hydrolysate by an oleaginous yeast Rhodotorula sp. IIP-33 using response surface methodology

Bandhu, Sheetal; Dasgupta, Diptarka; Akhter, Jawed; Kanaujia, Pankaj K.; Suman, Shankar; Agrawal, Deepti; Kaul, Savita; Adhikari, Dilip K.; Ghosh, Debashish

doi:10.1186/2193-1801-3-691

Cited by 23 publications

(11 citation statements)

References 19 publications

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“…According to the ANOVA results, the (NH 4 ) 2 SO 4 concentration showed a significant negative effect on lipid concentration and lipid content (data not shown). This was also observed by Bandhu et al [43] who found from BBD that (NH 4 ) 2 SO 4 concentration had significant negative effect on lipid concentration. Consequently, a decrease of the (NH 4 ) 2 SO 4 concentration led to enhanced lipid concentration together with lipid content.…”

Section: Optimization Of the Concentration Of (Nh 4 ) 2 So 4 Kh 2 Psupporting

confidence: 82%

Lipid Production from Sugarcane Top Hydrolysate and Crude Glycerol with Rhodosporidiobolus fluvialis Using a Two-Stage Batch-Cultivation Strategy with Separate Optimization of Each Stage

et al. 2020

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Lipids from oleaginous microorganisms, including oleaginous yeasts, are recognized as feedstock for biodiesel production. A production process development of these organisms is necessary to bring lipid feedstock production up to the industrial scale. This study aimed to enhance lipid production of low-cost substrates, namely sugarcane top and biodiesel-derived crude glycerol, by using a two-stage cultivation process with Rhodosporidiobolus fluvialis DMKU-SP314. In the first stage, sugarcane top hydrolysate was used for cell propagation, and in the second stage, cells were suspended in a crude glycerol solution for lipid production. Optimization for high cell mass production in the first stage, and for high lipid production in the second stage, were performed separately using a one-factor-at-a-time methodology together with response surface methodology. Under optimum conditions in the first stage (sugarcane top hydrolysate broth containing; 43.18 g/L total reducing sugars, 2.58 g/L soy bean powder, 0.94 g/L (NH4)2SO4, 0.39 g/L KH2PO4 and 2.5 g/L MgSO4 7H2O, pH 6, 200 rpm, 28 °C and 48 h) and second stage (81.54 g/L crude glycerol, pH 5, 180 rpm, 27 °C and 196 h), a high lipid concentration of 15.85 g/L, a high cell mass of 21.07 g/L and a high lipid content of 73.04% dry cell mass were obtained.

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Section: Optimization Of the Concentration Of (Nh 4 ) 2 So 4 Kh 2 Psupporting

confidence: 82%

Lipid Production from Sugarcane Top Hydrolysate and Crude Glycerol with Rhodosporidiobolus fluvialis Using a Two-Stage Batch-Cultivation Strategy with Separate Optimization of Each Stage

et al. 2020

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“…This was mainly attributed to the presence of P and S in these mineral salts. Because, in the literature, it is stated that carbon source is directed to cell growth in the presence of excessive P and S but to lipid synthesis under P and S limited conditions (Bandhu et al, 2014;González-García et al, 2017;Ortucu et al, 2017;Elfeky et al, 2019). The experiments revealed that NaCl concentrations up to 5 g/L significantly stimulated lipid synthesis.…”

Section: Discussionmentioning

confidence: 99%

Lipid Production by Yarrowia lipolytica B9 Using Crude Glycerol as Carbon Source

Arslan¹,

Tasar²

2021

Commagene Journal of Biology

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The present study was carried out to optimize some culture conditions to increase lipid production from Yarrowia lipolytica B9 in crude glycerol-based medium. The experiments displayed that too high concentrations of ammonium sulfate, KH2PO4, and MgSO4 increased cell growth but inhibited lipid synthesis. In contrast, more synthesis of lipids was determined to be achived at high concentrations of NaCl. The optimum concentrations of glycerol, ammonium sulfate, KH2PO4, MgSO4, and NaCl for lipid synthesis were determined as 50, 3, 1.5, 1 and 5 g/L, respectively. The optimal incubation time for lipid synthesis was found to be 6 days. Lipid concentration of 2.69 g/L and lipid content of 60.1%, were reached under optimal culture conditions.

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“…The enzyme solution was added after the cassava residue solution was cooled to approximately 40°C and the pH was adjusted to 4.0. Samples were then maintained at 30°C on a 150 r min −1 shaker .…”

Section: Methodsmentioning

confidence: 99%

Effects of the liquid vapor oxygen transfer coefficient (k_Lα) on ethanol production from cassava residue and analysis of the fermentation kinetics

Deng

et al. 2018

Energy Science & Engineering

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Cassava residue is the solid waste generated from the production of tapioca starch and has considerable reuse value. In this study, cassava residue was examined as a hydrolysate for ethanol production, and the effects of different liquid phase oxygen transfer coefficients (k L α) on the production of ethanol from cassava residue hydrolysates were studied. Based on analyses of dissolved oxygen and a set of optimal experimental schemes, dynamic models of cell growth and product synthesis were optimized, using MATLAB. When k L α was 85, fermentation was optimal; the ethanol titer reached 23.14 g L −1 at 72 h and cell growth reached 6.23 g L −1 at 96 h. Additionally, experiments were performed according to the dissolved oxygen curve obtained under these conditions. The resulting ethanol titer was 24.43 g L −1 (72 h) and the cell mass was 6.45 g L −1 (96 h). 84

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Statistical design and optimization of single cell oil production from sugarcane bagasse hydrolysate by an oleaginous yeast Rhodotorula sp. IIP-33 using response surface methodology

Cited by 23 publications

References 19 publications

Lipid Production from Sugarcane Top Hydrolysate and Crude Glycerol with Rhodosporidiobolus fluvialis Using a Two-Stage Batch-Cultivation Strategy with Separate Optimization of Each Stage

Lipid Production from Sugarcane Top Hydrolysate and Crude Glycerol with Rhodosporidiobolus fluvialis Using a Two-Stage Batch-Cultivation Strategy with Separate Optimization of Each Stage

Lipid Production by Yarrowia lipolytica B9 Using Crude Glycerol as Carbon Source

Effects of the liquid vapor oxygen transfer coefficient (k_Lα) on ethanol production from cassava residue and analysis of the fermentation kinetics

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Statistical design and optimization of single cell oil production from sugarcane bagasse hydrolysate by an oleaginous yeast Rhodotorula sp. IIP-33 using response surface methodology

Cited by 23 publications

References 19 publications

Lipid Production from Sugarcane Top Hydrolysate and Crude Glycerol with Rhodosporidiobolus fluvialis Using a Two-Stage Batch-Cultivation Strategy with Separate Optimization of Each Stage

Lipid Production from Sugarcane Top Hydrolysate and Crude Glycerol with Rhodosporidiobolus fluvialis Using a Two-Stage Batch-Cultivation Strategy with Separate Optimization of Each Stage

Lipid Production by Yarrowia lipolytica B9 Using Crude Glycerol as Carbon Source

Effects of the liquid vapor oxygen transfer coefficient (kLα) on ethanol production from cassava residue and analysis of the fermentation kinetics

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Effects of the liquid vapor oxygen transfer coefficient (k_Lα) on ethanol production from cassava residue and analysis of the fermentation kinetics