Stationary phase modulation in liquid chromatography through the serial coupling of columns: A review

Alvarez-Segura, T.; Torres-Lapası́o, J.R.; Ortiz-Bolsico, C.; Garcı́a-Alvarez-Coque, M.C.

doi:10.1016/j.aca.2016.03.040

Cited by 63 publications

(27 citation statements)

References 94 publications

(14 reference statements)

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“…A PFP column that exhibits hybrid stationary phase characteristics was used in a mixed-mode chromatography method and operated under both RPLC and HILIC conditions for SAMHSA-5 illicit drugs panel in urine (Clyde et al, 2015). Strategically combining RPLC, HILIC and PFP columns can capture and elute a wide range of both polar and hydrophilic metabolites in human matrices, covering a wider spectrum of the environmental organics chemical space (Tang et al, 2014, Alvarez-Segura et al, 2016, Shi et al, 2015). To achieve ideal results in a 2D-chromatography analysis, it is essential that the first and second columns are made of orthogonal phases enabling differing separations.…”

Section: Advances In Analytical Tools For Profiling the Environmenmentioning

confidence: 99%

Trends in the application of high-resolution mass spectrometry for human biomonitoring: An analytical primer to studying the environmental chemical space of the human exposome

Andra

Austin

Patel

et al. 2017

Environment International

124

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Global profiling of xenobiotics in human matrices in an untargeted mode is gaining attention for studying the environmental chemical space of the human exposome. Defined as the study of a comprehensive inclusion of environmental influences and associated biological responses, human exposome science is currently evolving out of the metabolomics science. In analogy to the latter, the development and applications of high resolution mass spectrometry (HRMS) has shown potential and promise to greatly expand our ability to capture the broad spectrum of environmental chemicals in exposome studies. HRMS can perform both untargeted and targeted analysis because of its capability of full- and/or tandem-mass spectrum acquisition at high mass accuracy with good sensitivity. The collected data from target, suspect and non-target screening can be used not only for the identification of environmental chemical contaminants in human matrices prospectively but also retrospectively. This review covers recent trends and advances in this field. We focus on advances and applications of HRMS in human biomonitoring studies, and data acquisition and mining. The acquired insights provide stepping stones to improve understanding of the human exposome by applying HRMS, and the challenges and prospects for future research.

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Section: Advances In Analytical Tools For Profiling the Environmenmentioning

confidence: 99%

Trends in the application of high-resolution mass spectrometry for human biomonitoring: An analytical primer to studying the environmental chemical space of the human exposome

Andra

Austin

Patel

et al. 2017

Environment International

124

View full text Add to dashboard Cite

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“…Good summaries of serial couplings have recently been published in comprehensive reviews by Haggarty and Burgess and Alvarez‐Segura et al . . The second type of setup (tandem LC) uses first a trap column to divide the sample composition into hydrophilic and hydrophobic compounds.…”

Section: Introductionmentioning

confidence: 99%

Tandem HILIC‐RP liquid chromatography for increased polarity coverage in food analysis

et al. 2018

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Comprehensive non-targeted analysis of food products normally requires two complementary chromatographic runs to achieve maximum compound coverage. In this study, we present a sensitive tandem-LC method, which combines RP and HILIC separation in a single run. The setup consists of a C18 trap column and two subsequently coupled analytical columns (HILIC and C18) which are operated in parallel. First, hydrophobic compounds are retained on the RP trap column while rather hydrophilic compounds are directly transferred onto a HILIC phase. Next, the pre-fractionated sample composition is analyzed by HILIC or RP chromatography, respectively. The presented setup allows individual and independent gradient elution as well as interfacing with mass spectrometry. The performance of the method has been proven by means of food relevant standards and analysis of complex food samples (e.g. red wine, meat extract). The simple and robust setup provides high flexibility in the selection of column combinations and does not require sophisticated instrumental setups or software. The method significantly increases the covered polarity range compared to classical one-dimensional chromatography. Our results indicate that tandem LC is a valuable and universal tool in the non-targeted screening of various types of complex food samples.

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“…The chromatographic column selection is mainly based on the hydrophobicity, hydrophilicity, molecular size (shape and size), charge (ionic exchange), dissociation (pKa), and so on . Type of chromatographic column packing, particle shape, particle size, specific surface area, pore size, ratio of bonding phase (carbon content), end group closure, column diameter, and length can all affect chromatographic separation effects . Several chromatographic column characterization systems have been reported by now, such as (a) the Snyder/Dolan approach (also called the PQRI procedure) is considered as the most comprehensive method for chromatographic column characterization .…”

Section: Introductionmentioning

confidence: 99%

Column selection approach for related substances determination of progesterone by high‐performance liquid chromatography

et al. 2018

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In this study, HPLC-MS/MS with triple-quadrupole tandem mass spectrometer was used to analyze the base-catalyzed thermal degradation derivative of progesterone in Chinese Pharmacopoeia. The full-scan spectrometry revealed that the degradation product was an isomer of progesterone, it was proposed as impurity M ((17α)-pregn-4-ene-3,20-dione) in European Pharmacopoeia, and both the derivative and progesterone were neutral molecules. Four kinds of chromatographic column characterization databases including PQRI database using Snyder/Dolan method, the USP chromatographic column database using the SRM 870 tests, the Tanaka/Euerby approach within the ACD program, and the Hoogmartens approach were compared. Combing the principles of column characterization databases with the system requirement of progesterone-related substances testing, a suitable PQRI-based method was finally set up for progesterone-related substances detecting. Our results indicated that PQRI system of Snyder/Dolan method was the most suitable system for related substances detection of progesterone.

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Stationary phase modulation in liquid chromatography through the serial coupling of columns: A review

Cited by 63 publications

References 94 publications

Trends in the application of high-resolution mass spectrometry for human biomonitoring: An analytical primer to studying the environmental chemical space of the human exposome

Trends in the application of high-resolution mass spectrometry for human biomonitoring: An analytical primer to studying the environmental chemical space of the human exposome

Tandem HILIC‐RP liquid chromatography for increased polarity coverage in food analysis

Column selection approach for related substances determination of progesterone by high‐performance liquid chromatography

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