Static time‐of‐flight secondary ion mass spectrometry imaging of freeze‐fractured, frozen‐hydrated biological membranes

Pacholski, Michaeleen L.; Cannon, Donald M.; Ewing, Andrew G.; Winograd, Nicholas

doi:10.1002/(sici)1097-0231(19980930)12:18<1232::aid-rcm319>3.3.co;2-7

Cited by 28 publications

(55 citation statements)

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“…This drawback has been partially worked around by the use of a tandem mass spectrometer, but in that particular case, the lateral resolution was limited to several hundreds of microns 7. The TOF‐SIMS technique, which by comparison is limited to ions like the phosphatidylcholine head group8 at m / z 184, can achieve a lateral resolution approaching a few tens of nanometers by using focused ion beams. Historically, the ion sources commonly used in TOF‐SIMS experiments have been Ga + or In + liquid metal ions guns (LMIGs);9 these provide excellent lateral resolutions but poor desorption efficiencies.…”

Section: Introductionmentioning

confidence: 99%

Biological tissue imaging with time‐of‐flight secondary ion mass spectrometry and cluster ion sources

2005

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Time-of-flight secondary ion mass spectrometry (TOF-SIMS) using liquid metal ion guns (LMIGs) is now sensitive enough to produce molecular-ion images directly from biological tissue samples. Primary cluster ions strike a spot on the sample to produce a mass spectrum. An image of this sample is achieved by rastering the irradiated point over the sample surface. The use of secondary ion mass spectrometry for mapping biological tissue surfaces provides unique analytical capabilities; in particular, it enables in a single acquisition a large variety of biological compounds to be localised on a micrometer scale and scrutinised for colocalisations. Without any treatment of the sample, this method is fully compatible with subsequent and complementary analyses like fluorescence microscopy, histochemical staining, or even matrix-assisted laser desorption/ionisation imaging. Basic physical concepts, required instrumentation (ion source and mass analyzer), sample preparation methods, image acquisition, image processing, and emerging biological applications will be described and discussed.

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Section: Introductionmentioning

confidence: 99%

Biological tissue imaging with time‐of‐flight secondary ion mass spectrometry and cluster ion sources

2005

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“…The secondary ion mass spectrometry (SIMS) is also a powerful technique for structural characterization and imaging for various kinds of samples, including biological tissues 15–18. The most popular primary ion source is the liquid metal ion source, which can produce a metal ion beam, such as Ga + .…”

Section: Introductionmentioning

confidence: 99%

“…In general, SIMS mass spectra of biological tissues were composed of atomic ions and low mass fragment ions. For example, the membrane lipids, phosphatidylcholines (PCs) were detected as PC head group fragment at m / z 184, and intact molecular ions are absent 10, 17. Thus, information of fatty acid residues in PCs can hardly be obtained.…”

Section: Introductionmentioning

confidence: 99%

Direct analysis of lipids in mouse brain using electrospray droplet impact/SIMS

et al. 2010

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Electrospray droplet impact (EDI)/secondary ion mass spectrometry (SIMS) is a new desorption/ionization technique for mass spectrometry in which highly charged water clusters produced from the atmospheric-pressure electrospray are accelerated in vacuum by 10 kV and impact the sample deposited on the metal substrate. EDI/SIMS was shown to enhance intact molecular ion formation dramatically compared to conventional SIMS. EDI/SIMS has been successfully applied to the analysis of mouse brain without any sample preparation. Five types of lipids, i.e. phosphatidylcholine (PC), phosphatidylserine, phosphatidylinositol (PI), galactocerebroside (GC) and sulfatide (ST), were readily detected from mouse brain section. In addition, by EDI/SIMS, six different regions of the mouse brain (cerebral cortex, corpus callosum, striatum, medulla oblongata, cerebellar cortex and cerebellar medulla) were examined. While GCs and STs were found to be rich in white matter, PIs were rich in gray matter.

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“…Historically, mapping of inorganic and a few low molecular weight (MW) organic compounds has been accomplished at nanometer resolution using secondary ion mass spectrometry (SIMS) 1. Static SIMS has been successfully employed with Cs + or Ga + primary ion beams as desorption probes to image various tissue samples and has proven to be a powerful method to investigate lipid membrane composition as well as steroid concentrations 2, 5. Because of the ion energetics involved, usually only fragments of the targeted compounds are imaged using SIMS.…”

Section: Introductionmentioning

confidence: 99%

Instrument design and characterization for high resolution MALDI‐MS imaging of tissue sections

2007

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In previous work, we have reported using a MALDI imaging time-of-flight mass spectrometer for the detection of protein ions from tissue sections with spatial resolution of 25 microm. We present here imaging mass spectrometry results obtained with a high-resolution scanning MALDI time-of-flight mass spectrometer, equipped with a coaxial laser illumination ion source, capable of achieving irradiation areas as small as 40 microm(2) (ca 7 microm diameter). MALDI-generated analyte ion signals from these very small irradiation volumes can be observed in a molecular weight range up to 27,000. High-resolution imaging mass spectrometry images were successfully generated from matrix thin film samples and tissue sections with scanning resolutions at and below 10 microm. This work also provides fundamental characterization of the ion signal dependence as a function of various focus and fluence parameters that will be required for extension to tissue imaging at the subcellular level.

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Static time‐of‐flight secondary ion mass spectrometry imaging of freeze‐fractured, frozen‐hydrated biological membranes

Cited by 28 publications

References 0 publications

Biological tissue imaging with time‐of‐flight secondary ion mass spectrometry and cluster ion sources

Biological tissue imaging with time‐of‐flight secondary ion mass spectrometry and cluster ion sources

Direct analysis of lipids in mouse brain using electrospray droplet impact/SIMS

Instrument design and characterization for high resolution MALDI‐MS imaging of tissue sections

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