Static magnetic field enhances synthesis and secretion of membrane-derived microvesicles (MVs) rich in VEGF and BMP-2 in equine adipose-derived stromal cells (EqASCs)—a new approach in veterinary regenerative medicine

Abstract

The aim of this work study was to evaluate the cytophysiological activity of equine adipose-derived stem cells (ASCs) cultured under conditions of static magnetic field. Investigated cells were exposed to a static magnetic field (MF) with the intensity of 0.5 T. In order to investigate the effects of magnetic field on stem cell signaling, the localization and density and content of microvesicles (MVs) as well as morphology, ultrastructure, and proliferation rate of equine ASCs were evaluated. Results showed that potential of equine adipose-derived mesenchymal stem cells was accelerated when magnetic field was applied. Resazurin-based assay indicated that the cells cultured in the magnetic field reached the population doubling time earlier and colony-forming potential of equine ASCs was higher when cells were cultured under magnetic field conditions. Morphological and ultrastructural examination of equine ASCs showed that the exposure to magnetic field did not cause any significant changes in cell morphology whereas the polarity of the cells was observed under the magnetic field conditions in ultrastructural examinations. Exposition to MF resulted in a considerable increase in the number of secreted MVs—we have clearly observed the differences between the numbers of MVs shed from the cells cultured under MF in comparison to the control culture and were rich in growth factors. Microvesicles derived from ASCs cultured in the MF condition might be utilized in the stem cell-based treatment of equine musculoskeletal disorders and tendon injuries.

Altered expression of CD63 and exosomes in scleroderma dermal fibroblasts

“…Prior to euthanasia, blood samples of mice were collected and centrifuged for serum separation. Exosomes were isolated by PureExo isolation kit (101Bio, Palo Alto, CA, USA) [19].…”

The effect of low static magnetic field on osteogenic and adipogenic differentiation potential of human adipose stromal/stem cells

“…Colony-forming unit fibroblast (CFU-F) potential of single cells was determined by plating cells with a density of 1000 cells/well on a 6-well plate for 14 days and analyzed as previously described [18].…”

“…As it has been shown, ASCs release MVs, which contain a wide range of molecules, including cytokines, growth factors, and microRNA (miRNA). They are involved in the improvement of donor cells response, proliferation and differentiation potential [17,18].…”

Combination of resveratrol and 5‐azacytydine improves osteogenesis of metabolic syndrome mesenchymal stem cells

“…Moreover, ASCs have the ability to differentiate into adipocytes, myocytes, chondrocytes and osteoblasts, which underlines their potential utility in future cell‐based therapies. The pro‐regenerative properties of ASCs are explained by their paracrine and autocrine activities based on the secretion of membrane‐derived extracellular vesicles (ExMVs), which are known to play a critical role in intracellular signalling 15, 16. ExMVs were demonstrated to contain a broad range of growth factors, including vascular endothelial growth factors, fibroblast growth factors and transforming growth factor‐β—all of which are crucial in the treatment of MetS 17.…”