Static and dynamic model fluorescence quenching of laser dye by carbon tetrachloride in binary mixtures

Kadadevarmath, Jagadish S.; Malimath, G.H.; Melavanki, Raveendra; Patil, N. R.

doi:10.1016/j.saa.2013.08.053

Cited by 42 publications

(14 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The two dyes revealed different baseline fluorescence intensities and slope magnitudes, thus resulting in variations in fluorescent intensities at similar time points within both time lapses. Although the relationship between dye concentration and fluorescence intensity is initially linear40, very high concentrations of these dyes can result in a decrease of fluorescence intensity as a result of dynamic quenching, an effect described by the Stern-Volmer equation in which excited chromophore molecules will interact with each other and lose energy through processes other than fluorescent emission41. Our testing of logarithmic concentrations of these dyes and measuring their emitted fluorescence through ImageJ ensured that the concentrations used for FU and TR here did not exhibit dynamic quenching.…”

Section: Discussionmentioning

confidence: 99%

Differential Canalograms Detect Outflow Changes from Trabecular Micro-Bypass Stents and Ab Interno Trabeculectomy

Parikh

Loewen

Roy

et al. 2016

Sci Rep

View full text Add to dashboard Cite

Recently introduced microincisional glaucoma surgeries that enhance conventional outflow offer a favorable risk profile over traditional surgeries, but can be unpredictable. Two paramount challenges are the lack of an adequate training model for angle surgeries and the absence of an intraoperative quantification of surgical success. To address both, we developed an ex vivo training system and a differential, quantitative canalography method that uses slope-adjusted fluorescence intensities of two different chromophores to avoid quenching. We assessed outflow enhancement by trabecular micro-bypass (TMB) implantation or by ab interno trabeculectomy (AIT). In this porcine model, TMB resulted in an insignificant (p > 0.05) outflow increase of 13 ± 5%, 14 ± 8%, 9 ± 3%, and 24 ± 9% in the inferonasal, superonasal, superotemporal, and inferotemporal quadrant, respectively. AIT caused a 100 ± 50% (p = 0.002), 75 ± 28% (p = 0.002), 19 ± 8%, and 40 ± 21% increase in those quadrants. The direct gonioscopy and tactile feedback provided a surgical experience that was very similar to that in human patients. Despite the more narrow and discontinuous circumferential drainage elements in the pig with potential for underperformance or partial stent obstruction, unequivocal patterns of focal outflow enhancement by TMB were seen in this training model. AIT achieved extensive access to outflow pathways beyond the surgical site itself.

show abstract

Section: Discussionmentioning

confidence: 99%

Differential Canalograms Detect Outflow Changes from Trabecular Micro-Bypass Stents and Ab Interno Trabeculectomy

Parikh

Loewen

Roy

et al. 2016

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Although the relationship between dye concentration and fluorescence intensity is initially linear 38 , very high concentrations of these dyes can result in a decrease of fluorescence intensity as a result of dynamic quenching, an effect described by the Stern-Volmer equation in which excited chromophore molecules will interact with each other and lose energy through processes other than fluorescent emission. 39 Our testing of logarithmic concentrations of these dyes and measuring their emitted fluorescence through ImageJ ensured that the concentrations used for FU and TR here did not exhibit dynamic quenching. A normalization coefficient corrected TR values to match FU thereby allowing a direct comparison of flow rates before and after each intervention.…”

Section: Discussionmentioning

confidence: 99%

Differential Canalograms Detect Outflow Changes from Trabecular Micro-Bypass Stents and Ab Interno Trabeculectomy

Parikh

Loewen

Roy

et al. 2016

Preprint

View full text Add to dashboard Cite

The increasing prevalence of glaucoma, a leading cause of blindness, makes the development of safer and more effective treatment more urgent. Recently introduced microincisional glaucoma surgeries that enhance conventional outflow offer a favorable risk profile but can be unpredictable. Two paramount challenges are the lack of an adequate surgical training model for new surgeries and the absence of pre-or intraoperative guidance to sites of reduced flow.To address both, we developed an ex vivo training system and a differential, quantitative canalography method to assess outflow enhancement by trabecular micro-bypass (TMB) implantation or by ab interno trabeculectomy (AIT). TMB resulted in insignificant (p>0.05) outflow increases of 13±5%, 14±8%, 9±3%, and 24±9% in the inferonasal, superonasal, superotemporal, and inferotemporal quadrants. AIT caused a 100±50% (p=0.002), 75±28% (p=0.002), 19±8%, and 40±21% increase in those quadrants. AIT eyes had a 7.5 (p=0.01), 5.7 (p=0.004), 2.3, and 1.8-fold greater outflow enhancement than matching quadrants of paired TMB-implanted eyes. Quantitative canalography demonstrated that TMB, when successful, provided focal outflow enhancements, while AIT achieved a more extensive access to outflow pathways including and beyond the surgical site itself.All rights reserved. No reuse allowed without permission.(which was not peer-reviewed) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity.The copyright holder for this preprint . http://dx

show abstract

“…The fluorescence quenching of organic molecules including especially the coumarine derivatives, such as 4-(5-methyl-3-furan-2-ylbenzofuran-2-yl)-7-methyl-chromen-2-one (MFBMC) [78], 4-(5-chloro-3-furan-2-yl benzofuran-2-yl)-6-methyl-2H-chromen-2-one (ClFBMC) [79] or a laser dye compound namely 4,4‴-Bis (2-butyloctyl-oxy)-p-quaterphenyl [BIBUQ] [80] in binary solvent mixtures (benzene-acetonitrile, 1,4-dioxane-acetonitrile) by quenchers like aniline, carbon tetrachloride etc. has been investigated by using steady-state and lifetime measurements.…”

Section: Fluorescence Quenching In Binary Solvent Mixturesmentioning

confidence: 99%

“…Melavanki and coauthors have studied comparatively the effect of different quenchers on the photo-optical behavior of many coumarine derivatives in binary solvent mixtures [80][81][82] and in pure solvents [83]. These fluorophores in microheterogeneous environment show a specific photophysical behavior.…”

Section: Fluorescence Quenching In Binary Solvent Mixturesmentioning

confidence: 99%

Investigations of absorption and emission spectral data in mixed liquid media. A short review of recent literature

Homocianu¹,

Airinei²

2015

Journal of Molecular Liquids

View full text Add to dashboard Cite

Static and dynamic model fluorescence quenching of laser dye by carbon tetrachloride in binary mixtures

Cited by 42 publications

References 20 publications

Differential Canalograms Detect Outflow Changes from Trabecular Micro-Bypass Stents and Ab Interno Trabeculectomy

Differential Canalograms Detect Outflow Changes from Trabecular Micro-Bypass Stents and Ab Interno Trabeculectomy

Differential Canalograms Detect Outflow Changes from Trabecular Micro-Bypass Stents and Ab Interno Trabeculectomy

Investigations of absorption and emission spectral data in mixed liquid media. A short review of recent literature

Contact Info

Product

Resources

About