Statherin and Histatin 1 Reduce Parotid Saliva-Promoted &lt;i&gt;Streptococcus mutans&lt;/i&gt; Strain MT8148 Adhesion to Hydroxyapatite Surfaces

Shimotoyodome, Akira; Kobayashi, Hisataka; Tokimitsu, Ichiro; Mutoh, T.; Takaesu, Yoshinori

doi:10.1159/000094286

Cited by 53 publications

(50 citation statements)

References 54 publications

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“…A small layer of isopropanol is added to the top of the gel prior to polymerization to straighten the level of the gel [11][12][13].…”

Section: Estimation Of Salivary Proline Rich Proteinsmentioning

confidence: 99%

Salivary proteins as biomarkers in dental caries: In vivo study

Nireeksha¹,

Hegde²,

N³

et al. 2017

Dent Oral Craniofac Res

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The aim of the study was to determine the relationship between salivary proteins and dental caries. 80 healthy adults were further divided into caries free (N=20) and caries active (N=60) based on DMFT index. Caries active was further divided into group I (D=1-3), group II (D=4-10), group III (D= >10).Saliva samples collected were further analysed for salivary total protein levels, salivary IgA levels, salivary mucin levels, salivary albumin/globulin ratio, salivary C-reactive protein levels and proline rich proteins. The data was then statistically analysed using one way ANOVA test and Post Hoc Tukey's test. P value < 0.05 was considered as statistically significant. Study showed significantly high levels of salivary total protein, salivary IgA and salivary albumin globulin ratio in caries free group. Salivary mucin and salivary C-reactive protein levels were significantly high in caries active group. 95% proline rich protein bands were present in caries free group, whereas caries active group showed 65% proline rich protein bands. This study adds to better understanding of salivary components and their role in dental caries. Modulation of these proteins could play an important role in determining the onset and progression of caries, opening a new avenue of caries prevention.

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“…A small layer of isopropanol is added to the top of the gel prior to polymerization to straighten the level of the gel [11][12][13].…”

Section: Estimation Of Salivary Proline Rich Proteinsmentioning

confidence: 99%

Salivary proteins as biomarkers in dental caries: In vivo study

Nireeksha¹,

Hegde²,

N³

et al. 2017

Dent Oral Craniofac Res

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“…In the present study, the surface hydrophilization with phosphorylated PEG derivatives was used for preventing saliva-promoted bacterial adhesion on hydroxyapatite. Pretreatment of the HA surface with MDP-PEG copolymers prior to saliva incubation hydrophilized the surface and thereby reduced salivary protein adsorption and saliva-promoted bacterial attachment to HA surfaces, mimicking statherin and histatin 1 (28).…”

Section: Discussionmentioning

confidence: 99%

“…Dental biofilm formation after thorough teeth cleaning was positively correlated with both salivary glycoprotein content and S. mutans adhesion onto saliva-coated HA surfaces. A higher glycoprotein content forms an "adhesive" conditioning film (acquired pellicle) that promotes a higher rate of bacterial attachment onto HA surfaces in vitro (28).…”

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confidence: 99%

“…In contrast to salivary glycoprotein, some small salivary proteins, statherin (26) and histatin 1 (20), have an inhibitory effect on saliva-mediated S. mutans adhesion in vitro (28). These non-S. mutans-binding phosphoproteins reduce S. mutans adhesion by competitively inhibiting the adsorption of S. mutans-binding salivary glycoproteins to HA surfaces.…”

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confidence: 99%

“…To design statherin or histatin 1-like compounds, we considered the following two factors. (i) Our previous study showed that the phosphate and carboxyl groups in the Nterminal domain of statherin and histatin 1, which are capable of binding to HA surfaces through charge interactions, were essential for their inhibitory effects on bacterial adhesion, as well as noncharged or weakly charged amino acid domains (28). (ii) The studies thus far have proved that a noncharged hydrophilic surface, which may result from treatment with, for example, polyethylene glycol (PEG) derivatives, prevent bacterial adhesion effectively (12,21,22).…”

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confidence: 99%

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Reduction of Streptococcus mutans Adherence and Dental Biofilm Formation by Surface Treatment with Phosphorylated Polyethylene Glycol

Shimotoyodome

Koudate

Kobayashi

et al. 2007

Antimicrob Agents Chemother

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Initial attachment of the cariogenic Streptococcus mutans onto dental enamel is largely promoted by the adsorption of specific salivary proteins on enamel surface. Some phosphorylated salivary proteins were found to reduce S. mutans adhesion by competitively inhibiting the adsorption of S. mutans-binding salivary glycoproteins to hydroxyapatite (HA). The aim of this study was to develop antiadherence compounds for preventing dental biofilm development. We synthesized phosphorylated polyethylene glycol (PEG) derivatives and examined the possibility of surface pretreatment with them for preventing S. mutans adhesion in vitro and dental biofilm formation in vivo. Pretreatment of the HA surface with methacryloyloxydecyl phosphate (MDP)-PEG prior to saliva incubation hydrophilized the surface and thereby reduced salivary protein adsorption and saliva-promoted bacterial attachment to HA. However, when MDP-PEG was added to the saliva-pretreated HA (S-HA) surface, its inhibitory effect on bacterial binding was completely diminished. S. mutans adhesion onto S-HA was successfully reduced by treatment of the surface with pyrophosphate (PP), which desorbs salivary components from S-HA. Treatment of S-HA surfaces with MDP-PEG plus PP completely inhibited salivapromoted S. mutans adhesion even when followed by additional saliva treatment. Finally, mouthwash with MDP-PEG plus PP prevented de novo biofilm development after thorough teeth cleaning in humans compared to either water or PP alone. We conclude that MDP-PEG plus PP has the potential for use as an antiadherence agent that prevents dental biofilm development.Dental plaque, a complex microbial biofilm, is the primary etiologic factor in dental caries. Colonization of enamel surfaces by the cariogenic bacterium Streptococcus mutans is thought to be initiated by attachment to a saliva-derived conditioning film, the acquired enamel pellicle (9). The acquired enamel pellicle is formed largely by adsorption of heterogeneous salivary proteins (1, 18) onto dental enamel (ϳ98% [wt/wt] hydroxyapatite [HA]) and promotes the adhesion of S. mutans by specific (2, 17) and nonspecific (4) mechanisms.Saliva contains a multitude of proteins that contribute to oral microbial ecology and biofilm formation (6,16,32). A variety of salivary proteins have been shown to modulate bacterial adhesion onto HA surfaces in vitro. Adsorption of specific salivary proteins, such as acidic proline-rich proteins (8) and agglutinin (3), promotes the adhesion of S. mutans onto HA surfaces by providing ligands for bacterial attachment. Many studies thus far have suggested that an initial bacterial adhesion promoted by salivary protein adsorption onto the enamel surface contributes to facilitate dental biofilm development.Recently, we reported the clinical relevance of saliva-promoted S. mutans adhesion in both de novo dental biofilm development and caries experience (29). Dental biofilm formation after thorough teeth cleaning was positively correlated with both salivary glycoprotein content and S. mutans a...

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Molecular Simulation of Statherin Adsorption on Hydroxyapatite (001) Surface

Ding

Zeng

Luo

et al. 2022

Adv Materials Inter

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Because of the high prevalence and ability to rapidly break the enamel, [6] it is important to develop the effective treatment and prevention methods. Unfortunately, up to now, there is no mature standard method for the treatment of enamel erosion. Although the use of fluorinated dental products can inhibit the occurrence of enamel erosion to a certain extent, [7] yet it does not solve the problem from the root.There are several natural protective mechanisms for tooth demineralization in human saliva and the formation of acquired pellicle (AP) is one of them. AP is a membrane formed onto the surface of teeth in saliva, including some lipids and glycoproteins. [8] As a natural barrier, AP can prevent the direct contact between enamel and food acid. Under acidic condition, the diffusion rate of phosphate and calcium ions (Ca 2+ ) into surrounding saliva decreases significantly, thus preventing enamel demineralization. [9] Statherin (SN) is an important protein for AP, which can selectively adsorb on enamel surface. [10] It is composed of 43 amino acids and has a high degree of structural and charge asymmetry. SN can effectively inhibit the excessive precipitation of calcium phosphate and maintain saliva as a supersaturated solution of Ca 2+ and phosphorus ions. It can also promote enamel remineralization when enamel is demineralized. [11] Early results showed that the high negatively charged density at N-terminus of SN and the α-helix structure composed of the first 15 residues were the key factors for its adsorption on the HAP surface. [11a] In addition, its N-terminus pre-hexapeptide D-pS-pS-EEK (pS denotes the phosphoserine) could also form α-helix structure and adsorb on HAP, and its variant DDDEEK also had similar adsorption capacity. [12] However, a molecular understanding of adsorption process of SN on HAP surface is lacking. Currently, some researchers have attempted to utilize the molecular-level simulation method to study the adsorption mechanism of protein on HAP surface. Hoff et al. [13] used molecular dynamics (MD) method to quantify the binding free energies of 20 natural amino acids and citrate ions on HAP surface, and found that citrate has the highest affinity to HAP, followed by charged residues, while amino acids without ionic side groups exhibit weak binding to HAP. Basu et al. [14] investigated the influence of electrical stimuli on fibronectin (FN) adsorption on HAP surface using the MD simulation method, and found that the charged residues contributed majorly to FN adsorption, and the adsorption was significantly affected by electric field strength. Moreover, Statherin (SN) is an important protein that can adsorb on enamel to maintain calcium ions balance and promote biomineralization in the human oral cavity. In this work, parallel tempering Monte Carlo simulation and all-atom molecular dynamics simulation are combined to study the behavior of SN adsorbed on the hydroxyapatite (HAP) (001) surface. The results indicate that SN can "anchor" on HAP (001) surface by its Asp1 and Sep2 with "head...

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Statherin and Histatin 1 Reduce Parotid Saliva-Promoted <i>Streptococcus mutans</i> Strain MT8148 Adhesion to Hydroxyapatite Surfaces

Cited by 53 publications

References 54 publications

Salivary proteins as biomarkers in dental caries: In vivo study

Salivary proteins as biomarkers in dental caries: In vivo study

Reduction of Streptococcus mutans Adherence and Dental Biofilm Formation by Surface Treatment with Phosphorylated Polyethylene Glycol

Molecular Simulation of Statherin Adsorption on Hydroxyapatite (001) Surface

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