States and challenges for high-value biohythane production from waste biomass by dark fermentation technology

Expression of different types of [FeFe]-hydrogenase genes in bacteria isolated from a population of a bio-hydrogen pilot-scale plant

“…A particular interest concerning the precise mode and timing of hydrogen production is relevant for bio-hytane production, a very promising fuel that can be obtained in two-phase dark fermentation plants by suitably combining bio-hydrogen and bio-methane streams [4].…”

Biofuels from food processing wastes

“…The main advantage of the bio-hythane process is the low cost and high energy yield. The key bioengineering strategy for hythane production is to control the acidogenesis, acetogenesis and methanogenesis stages to reach a hydrogen/methane ratio suitable for hythane [16,18 ].…”

“…In the first stage, operation parameters (pH of 5.5-6.5, thermophilic conditions) favouring the growth of hydrogenogenic bacteria, but inhibiting methanogenic bacteria need to be well controlled, while higher pH (7.0-7.5) and mesophilic condition are used in the second methanogenic stage [18 ]. There are a few of update successful studies on the two-stage processes for biohythane production using palm oil effluent [20 ], fruit vegetable waste [21], and starch wastewater [22 ].…”

Deciphering acidogenic process towards biohydrogen, biohythane, and short chain fatty acids production: multi-output optimization strategy

“…The gaseous products of acidogenesis (i.e., H2 and CH4) are renewable and important energy carriers which can be used individually as well as in a blend. The individual limitations of H2 and CH4 could be overcome by blending them together in proportionate ratios to form biohythane (Liu et al, 2013; Venkata Mohan, 2015 2015;…”