State of the Art of Genetic Engineering in Potato: From the First Report to Its Future Potential

Nahirñak, Vanesa; Almasia, Natalia Inés; Gonzalez, Matías Nicolás; Massa, G; Oneto, Cecilia Andrea Décima; Feingold, Sérgio Enrique; Hopp, H. Esteban; Rovere, Cecilia Vázquez

doi:10.3389/fpls.2021.768233

Cited by 14 publications

(21 citation statements)

References 164 publications

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“…Due to numerous applications and interesting biology, potato cultivars are often selected as research models in both basic and applied research (Nahirñak et al 2022 ). The primary factors that make potato an attractive research model include the simplicity of potato cultivation both in the field and under laboratory conditions, including in vitro, and the availability of optimized protocols for protoplast isolation and tissue culture of roots and tubers, thereby enabling the implementation of modern gene/genetic technologies (for review, see Hameed et al 2018 ; Nahirñak et al 2022 ; Tiwari et al 2022 ). It should be noted that potato is one of the few species for which effective in vitro regeneration procedures from a single protoplast to a fully organized plant have been developed (Mao et al 2019 ; Rather et al 2022 ).…”

Section: Applications Of Crispr/cas In Potatomentioning

confidence: 99%

“…As the main representatives of tuber crops and vegetatively reproducing polyploids, potatoes are frequently selected to test the usefulness of the latest NBTs aimed at improving plant quality and productivity (Hameed et al 2018 ; Tussipkan and Manabayeva 2021 ; Sharma et al 2022 ). Among the potato tetraploid cultivars, Désirée is most commonly used in studies involving the application of CRISPR/Cas technologies (Nahirñak et al 2022 ; Tiwari et al 2022 ; Sharma et al 2022 ). For this cultivar, various research tools are currently available, ranging from numerous established protocols for genetic manipulation to large amounts of data for omics studies and the recently released SNP map (Sevestre et al 2020 ; Nahirñak et al 2022 ).…”

Section: Applications Of Crispr/cas In Potatomentioning

confidence: 99%

“…Among the potato tetraploid cultivars, Désirée is most commonly used in studies involving the application of CRISPR/Cas technologies (Nahirñak et al 2022 ; Tiwari et al 2022 ; Sharma et al 2022 ). For this cultivar, various research tools are currently available, ranging from numerous established protocols for genetic manipulation to large amounts of data for omics studies and the recently released SNP map (Sevestre et al 2020 ; Nahirñak et al 2022 ). However, Désirée is not an ideal candidate for testing genome editing approaches; the analyses of quantitative traits, the selection of candidate genes underlying these traits, and the screening of mutated genes are more complex and time-consuming in tetraploid cultivars (Nahirñak et al 2022 ; Sharma et al 2022 ).…”

Section: Applications Of Crispr/cas In Potatomentioning

confidence: 99%

“…For this cultivar, various research tools are currently available, ranging from numerous established protocols for genetic manipulation to large amounts of data for omics studies and the recently released SNP map (Sevestre et al 2020 ; Nahirñak et al 2022 ). However, Désirée is not an ideal candidate for testing genome editing approaches; the analyses of quantitative traits, the selection of candidate genes underlying these traits, and the screening of mutated genes are more complex and time-consuming in tetraploid cultivars (Nahirñak et al 2022 ; Sharma et al 2022 ). Therefore, potatoes with a low ploidy level, such as the wild species S. chacoense , and with reduced ploidy, such as artificially generated dihaploids (DHs), are good candidates for reverse genetics studies and testing CRISPR/Cas techniques (Brigneti et al 2004 ; Duangpan et al 2013 ; Butler et al 2015 , 2016 ).…”

Section: Applications Of Crispr/cas In Potatomentioning

confidence: 99%

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Recent advances and challenges in potato improvement using CRISPR/Cas genome editing

Chincinska

Miklaszewska

Sołtys‐Kalina

2022

Planta

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Main conclusion Genome editing using CRISPR/Cas technology improves the quality of potato as a food crop and enables its use as both a model plant in fundamental research and as a potential biofactory for producing valuable compounds for industrial applications. Abstract Potato (Solanum tuberosum L.) plays a significant role in ensuring global food and nutritional security. Tuber yield is negatively affected by biotic and abiotic stresses, and enzymatic browning and cold-induced sweetening significantly contribute to post-harvest quality losses. With the dual challenges of a growing population and a changing climate, potato enhancement is essential for its sustainable production. However, due to several characteristics of potato, including high levels of heterozygosity, tetrasomic inheritance, inbreeding depression, and self-incompatibility of diploid potato, conventional breeding practices are insufficient to achieve substantial trait improvement in tetraploid potato cultivars within a relatively short time. CRISPR/Cas-mediated genome editing has opened new possibilities to develop novel potato varieties with high commercialization potential. In this review, we summarize recent developments in optimizing CRISPR/Cas-based methods for potato genome editing, focusing on approaches addressing the challenging biology of this species. We also discuss the feasibility of obtaining transgene-free genome-edited potato varieties and explore different strategies to improve potato stress resistance, nutritional value, starch composition, and storage and processing characteristics. Altogether, this review provides insight into recent advances, possible bottlenecks, and future research directions in potato genome editing using CRISPR/Cas technology.

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Section: Applications Of Crispr/cas In Potatomentioning

confidence: 99%

Section: Applications Of Crispr/cas In Potatomentioning

confidence: 99%

Section: Applications Of Crispr/cas In Potatomentioning

confidence: 99%

Section: Applications Of Crispr/cas In Potatomentioning

confidence: 99%

See 2 more Smart Citations

Recent advances and challenges in potato improvement using CRISPR/Cas genome editing

Chincinska

Miklaszewska

Sołtys‐Kalina

2022

Planta

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“…CRISPR/cas9 avoids foreign DNA insertions in the plant genome, an important criterion in the development of crop varieties not subjected to the cumbersome GMO regulation process [26][27][28]34]. Moreover, the availability of the potato genome sequence [35] has facilitated the development of comparative genomic analyses and functional studies of candidate genes to improve several important traits in potato [36]. In this review we discuss the past developments, and future perspectives of nutritional enhancement in potato using transgenic technologies.…”

Section: Introductionmentioning

confidence: 99%

Transgenic Approaches for Nutritional Enhancement of Potato

Datir¹,

Regan²

2023

Advances in Root Vegetables Research

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Potatoes provide an excellent source of carbohydrates, minerals, vitamins, carotenoids, anthocyanins, and several other metabolites which play an important role in human nutrition. These bioactive compounds are effective in preventing diseases like cancer, diabetes, and heart-related issues. In addition to their industrial uses, potatoes are a major focus of genetic engineering programs for the modification of nutritional properties. Several important candidate genes operating in phenylpropanoid mechanism, ascorbic acid biosynthesis pathway, carbohydrate metabolism, steroidal glycoalkaloid biosynthesis pathway, and other-related metabolic steps have been cloned and characterized at the biochemical and molecular levels. Overexpression and down regulation of genes operating in these pathways has revealed important insights into improved nutritional quality. Expression of a transgene has successfully resulted in increasing carotenoids, anthocyanins, and vitamin content in transgenic tubers. Reduction in glycoalkaloid content, enzymatic browning, flesh color, and chipping quality has been achieved via modification of the genes involved in the respective biochemical pathway in potatoes. Transgenic approaches not only resulted in improved quality but also helped in understanding the biochemical and molecular mechanisms associated with the regulation of genes in these pathways. Although the commercialization of transgenic potatoes is still hindered by consumers approval and ethical restrictions, the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system holds promise as a non-transgenic alternative for developing nutritionally enhanced potatoes.

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Applications of biotechnology for enhancing the shelf life of horticultural crops

Mihrete,

Workie,

Bogale

2024

J of Sust Agri & Env

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Horticultural crops, encompassing fruits, vegetables, spices and herbs, play a critical role in providing nutrition and health‐promoting compounds. However, their limited storability challenges producers and exporters, resulting in significant postharvest losses. Traditional preservation methods like cold storage, controlled atmosphere storage and packaging techniques have been employed to prolong shelf life, but they have their constraints. Biotechnological interventions, notably genetic engineering, offer promising avenues to address these limitations. Genetic modifications target physiological processes such as ripening and ethylene production, enhancing resistance to postharvest diseases and improving nutritional profiles. For instance, genetically modified tomatoes with prolonged shelf life and reduced susceptibility to fungal infections showcase the potential of genetic engineering. Similarly, genetic modification has been successfully applied to various horticultural crops like apples, bananas and mushrooms, resulting in decreased browning and heightened disease resistance. Emerging technologies such as modified atmosphere packaging, edible coatings and nanoparticle treatments further augment efforts to extend shelf life. Despite their benefits, the debate surrounding genetically modified fruits and vegetables persists due to concerns regarding environmental impact, health implications and ethical considerations. This review offers insights into current practices and research endeavours aimed at enhancing the shelf life of horticultural crops through both traditional and biotechnological means, shedding light on opportunities and hurdles in this domain. Future directions include intensifying basic research to unravel molecular processes in harvested tissues, prioritising investigations that directly benefit consumers and developing sustainable and cost‐effective approaches for emerging technologies like modified atmosphere packaging, edible coatings and postharvest treatments.

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State of the Art of Genetic Engineering in Potato: From the First Report to Its Future Potential

Cited by 14 publications

References 164 publications

Recent advances and challenges in potato improvement using CRISPR/Cas genome editing

Recent advances and challenges in potato improvement using CRISPR/Cas genome editing

Transgenic Approaches for Nutritional Enhancement of Potato

Applications of biotechnology for enhancing the shelf life of horticultural crops

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