State-of-the-Art in CRISPR Technology and Engineering Drought, Salinity, and Thermo-tolerant crop plants

Chennakesavulu, Kunchapu; Singh, Harshita; Trivedi, Prabodh Kumar; Jain, Mukesh; Yadav, Shri Ram

doi:10.1007/s00299-021-02681-w

Cited by 33 publications

(23 citation statements)

References 143 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this article, we present a small snapshot of the utility of gene editing tools that can be brought to bear to further our knowledge of two DNA repair pathways, MMR and BER. The far-reaching implications of CRISPRbased gene editing go well beyond basic science research with roles in medicine and therapeutics [423], gene therapy [424], agriculture [425], advances in animal modeling [426], and cancer discovery [427]. CRISPR methods open the door to the personalized treatment of genetic diseases.…”

Section: Discussionmentioning

confidence: 99%

Exploiting DNA Endonucleases to Advance Mechanisms of DNA Repair

Thompson

Sobol

Prakash

2021

Biology

View full text Add to dashboard Cite

The earliest methods of genome editing, such as zinc-finger nucleases (ZFN) and transcription activator-like effector nucleases (TALENs), utilize customizable DNA-binding motifs to target the genome at specific loci. While these approaches provided sequence-specific gene-editing capacity, the laborious process of designing and synthesizing recombinant nucleases to recognize a specific target sequence, combined with limited target choices and poor editing efficiency, ultimately minimized the broad utility of these systems. The discovery of clustered regularly interspaced short palindromic repeat sequences (CRISPR) in Escherichia coli dates to 1987, yet it was another 20 years before CRISPR and the CRISPR-associated (Cas) proteins were identified as part of the microbial adaptive immune system, by targeting phage DNA, to fight bacteriophage reinfection. By 2013, CRISPR/Cas9 systems had been engineered to allow gene editing in mammalian cells. The ease of design, low cytotoxicity, and increased efficiency have made CRISPR/Cas9 and its related systems the designer nucleases of choice for many. In this review, we discuss the various CRISPR systems and their broad utility in genome manipulation. We will explore how CRISPR-controlled modifications have advanced our understanding of the mechanisms of genome stability, using the modulation of DNA repair genes as examples.

show abstract

Section: Discussionmentioning

confidence: 99%

Exploiting DNA Endonucleases to Advance Mechanisms of DNA Repair

Thompson

Sobol

Prakash

2021

Biology

View full text Add to dashboard Cite

show abstract

“…The CRISPR-Cas system is now increasingly used for developing edited crops due to its diverse applications in genome editing. Using the CRISPR-Cas, research groups have engineered several crop systems for disease resistance ( Schenke and Cai, 2020 ), drought, salinity, and thermotolerance ( Chennakesavulu et al, 2021 ). CRISPR-Cas is aiding in developing climate-ready crops ( Razzaq et al, 2021 ) and improving crop quality parameters such as appearance, palatability, nutritional components, and other preferred traits ( Liu Q et al, 2021 ).…”

Section: Development Of Biofortified Crops Through Crispr-cas Genome ...mentioning

confidence: 99%

CRISPR-Based Genome Editing for Nutrient Enrichment in Crops: A Promising Approach Toward Global Food Security

et al. 2022

View full text Add to dashboard Cite

The global malnutrition burden imparts long-term developmental, economic, social, and medical consequences to individuals, communities, and countries. The current developments in biotechnology have infused biofortification in several food crops to fight malnutrition. However, these methods are not sustainable and suffer from several limitations, which are being solved by the CRISPR-Cas-based system of genome editing. The pin-pointed approach of CRISPR-based genome editing has made it a top-notch method due to targeted gene editing, thus making it free from ethical issues faced by transgenic crops. The CRISPR-Cas genome-editing tool has been extensively used in crop improvement programs due to its more straightforward design, low methodology cost, high efficiency, good reproducibility, and quick cycle. The system is now being utilized in the biofortification of cereal crops such as rice, wheat, barley, and maize, including vegetable crops such as potato and tomato. The CRISPR-Cas-based crop genome editing has been utilized in imparting/producing qualitative enhancement in aroma, shelf life, sweetness, and quantitative improvement in starch, protein, gamma-aminobutyric acid (GABA), oleic acid, anthocyanin, phytic acid, gluten, and steroidal glycoalkaloid contents. Some varieties have even been modified to become disease and stress-resistant. Thus, the present review critically discusses CRISPR-Cas genome editing-based biofortification of crops for imparting nutraceutical properties.

show abstract

“…The multiplex CRISPR/Cas9 system in the regulation of abiotic stress tolerance has been thoroughly reviewed in oilseed rape (Chikkaputtaiah et al, 2017 ). In this paper, we provide an overview of CRISPR/Cas GE technology in genome editing in oilseed crops, including primary editing (PE), base editing (BE), tissue-specific editing (CRISPR-TSKO), epigenome editing, and inducible genome editing (CRISPR-IGE), which can help to obtain resistant varieties that can tolerate the deleterious effects of high-temperature stress (Chennakesavulu et al, 2021 ) and has three dimensions, including adoption, crRNA biogenesis, and interference (Gasiunas et al, 2012 ; Jinek et al, 2012 ). Synthetic 20-nucleotide guide crRNA or RNA (gRNA) and Cas proteins are introduced into plants via a plasmid.…”

Section: Mechanism Of Heat Stress Tolerance In Plantsmentioning

confidence: 99%

Adaptation Strategies to Improve the Resistance of Oilseed Crops to Heat Stress Under a Changing Climate: An Overview

et al. 2021

View full text Add to dashboard Cite

Temperature is one of the decisive environmental factors that is projected to increase by 1. 5°C over the next two decades due to climate change that may affect various agronomic characteristics, such as biomass production, phenology and physiology, and yield-contributing traits in oilseed crops. Oilseed crops such as soybean, sunflower, canola, peanut, cottonseed, coconut, palm oil, sesame, safflower, olive etc., are widely grown. Specific importance is the vulnerability of oil synthesis in these crops against the rise in climatic temperature, threatening the stability of yield and quality. The natural defense system in these crops cannot withstand the harmful impacts of heat stress, thus causing a considerable loss in seed and oil yield. Therefore, a proper understanding of underlying mechanisms of genotype-environment interactions that could affect oil synthesis pathways is a prime requirement in developing stable cultivars. Heat stress tolerance is a complex quantitative trait controlled by many genes and is challenging to study and characterize. However, heat tolerance studies to date have pointed to several sophisticated mechanisms to deal with the stress of high temperatures, including hormonal signaling pathways for sensing heat stimuli and acquiring tolerance to heat stress, maintaining membrane integrity, production of heat shock proteins (HSPs), removal of reactive oxygen species (ROS), assembly of antioxidants, accumulation of compatible solutes, modified gene expression to enable changes, intelligent agricultural technologies, and several other agronomic techniques for thriving and surviving. Manipulation of multiple genes responsible for thermo-tolerance and exploring their high expressions greatly impacts their potential application using CRISPR/Cas genome editing and OMICS technology. This review highlights the latest outcomes on the response and tolerance to heat stress at the cellular, organelle, and whole plant levels describing numerous approaches applied to enhance thermos-tolerance in oilseed crops. We are attempting to critically analyze the scattered existing approaches to temperature tolerance used in oilseeds as a whole, work toward extending studies into the field, and provide researchers and related parties with useful information to streamline their breeding programs so that they can seek new avenues and develop guidelines that will greatly enhance ongoing efforts to establish heat stress tolerance in oilseeds.

show abstract

State-of-the-Art in CRISPR Technology and Engineering Drought, Salinity, and Thermo-tolerant crop plants

Cited by 33 publications

References 143 publications

Exploiting DNA Endonucleases to Advance Mechanisms of DNA Repair

Exploiting DNA Endonucleases to Advance Mechanisms of DNA Repair

CRISPR-Based Genome Editing for Nutrient Enrichment in Crops: A Promising Approach Toward Global Food Security

Adaptation Strategies to Improve the Resistance of Oilseed Crops to Heat Stress Under a Changing Climate: An Overview

Contact Info

Product

Resources

About