State of the Antioxidant System and the Severity of Lipid-Peroxidation Processes in the Myocardium and Blood Plasma of Rats of Different Ages with Postinfarction Cardiosclerosis

Rebrova, Т. Yu.; Afanasiev, S. A.

doi:10.1134/s2079057021020132

Cited by 3 publications

(5 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Также можно предположить, что при введении токсических доз изадрина в клетке в ответ на воздействие данного агента повышается синтез активных форм кислорода ввиду интенсификации процессов энергообразования и вероятного повышения утечки электронов из электрон-транспортной цепи. Это согласуется с результатами наших ранее проведенных исследований, показавших, что с возрастом снижается активность антиоксидантных ферментов в ткани миокарда [9].…”

Section: Discussionunclassified

Respiration in isolated cardiomyocytes and microviscosity of their membranes in rats of different ages with heart failure

2023

Self Cite

View full text Add to dashboard Cite

Background. According to the latest epidemiological data, heart failure (HF) is diagnosed in 10% of adult population over 70 years old. However, currently this diagnosis is being increasingly made in young and middleaged people. The pathogenesis of HF may be based on a decrease in respiration in cardiomyocytes with age, which affects the function of energy-dependent processes in cells.Aim. To study respiration in cardiomyocytes and microviscosity of their membranes in rats aged 2 and 15 months with heart failure.Materials and methods. The study was carried out on male Wistar rats. The animals were divided into 4 groups: 2 groups of intact rats aged 2 and 15 months (n = 12) and 2 groups of animals of similar ages (n = 10) with a model of HF. In the latter, HF formed by day 28 after a double subcutaneous injection of isoproterenol hydrochloride at a dose of 170 mg / kg with an interval of 24 hours. Isolated cardiomyocytes were obtained from enzyme-washed rat hearts. Cell respiration was studied in a thermostated chamber in an incubation medium supplemented with phosphorylation (ADP) and oxidation (succinate) substrates. The respiratory control (RC) ratio was calculated by dividing V3 respiration state to V4. Membrane microviscosity characteristics were assessed by the eximerization coefficient of pyrene fluorescence in the areas of protein – lipid and lipid – lipid interactions.Results. RC in cardiomyocyte membranes of intact animals did not change with age. In 2-month-old rats with HF, respiratory control ratio (RCR) did not change compared with the intact age-matched controls. In 15-month-old rats with HF, there was a significant decrease in RC of cardiomyocytes (CM) compared with the intact animals of this age and 2-month-old rats with HF. An age-dependent decrease in the microviscosity of CM membranes in the areas of lipid – lipid interactions and no significant changes in the parameter at the sites of protein – lipid interactions were noted. In 2-month-old animals with HF, the microviscosity of CM membranes in the areas of protein – lipid interactions significantly decreased, and in 15-month-old rats it increased, compared with the intact controls. When carrying out an intergroup comparison, an age-dependent increase in the microviscosity of CM membranes in the areas of protein – lipid interactions and no differences in the parameter in the areas of lipid – lipid interactions were revealed.Conclusion. In the intact rats, the absence of significant changes in respiration with age was revealed. In the 15-month-old animals with HF, respiration in CM was significantly lower than in the intact controls and 2-monthold animals with HF. These changes may be due to the differences in the membrane microviscosity characteristics in different periods of ontogenesis.

show abstract

Section: Discussionunclassified

Respiration in isolated cardiomyocytes and microviscosity of their membranes in rats of different ages with heart failure

2023

Self Cite

View full text Add to dashboard Cite

show abstract

“…Отмеченное в группе животных 4-месячного возраста с ПИКС наиболее выраженное угнетение относительно ЛО контроля прироста амплитуды ПЭС сокращений при ЭСИ длительностью 0,2 и 0,25 с может быть следствием снижения Са 2+ -аккумулирующей способности СПР в результате постинфарктного ремоделирования. Причиной такого нарушения функционирования СПР может являться состояние липидного бислоя мембран и снижение активности Са 2+ -АТФазы в результате активации свободнорадикальных процессов при развитии сердечной недостаточности ишемического генеза [27,28]. ПИКС в меньшей степени повлиял на Са 2+ -аккумулирующую способность СПР у 12-и 24-месячных крыс.…”

Section: Discussionunclassified

“…Такой результат хорошо согласуется с ранее сделанным предположением о том, что процессы перекисного окисления, активирующиеся в процессе онтогенеза и ассоциированные со старением, не могут приобрести еще большую активность при формировании ПИКС у стареющих животных [28].…”

Section: Discussionunclassified

“…В ранее проведенных исследованиях мы показали, что у животных в возрасте 12 и 24 мес. происходит снижение образования первичных продуктов перекисного окисления липидов [24,28] и карбонильных производных перекисного окисления белков [24]. Низкая интенсивность процессов перекисного окисления липидов и белков у животных старших возрастных групп сохраняется и при постинфарктном ремоделировании [24,28].…”

Section: Discussionunclassified

“…происходит снижение образования первичных продуктов перекисного окисления липидов [24,28] и карбонильных производных перекисного окисления белков [24]. Низкая интенсивность процессов перекисного окисления липидов и белков у животных старших возрастных групп сохраняется и при постинфарктном ремоделировании [24,28]. Эти результаты позволяют предположить, что снижение интенсивности перекисной модификации липидов и белков при ПИКС у животных в возрасте 24 мес.…”

Section: Discussionunclassified

See 2 more Smart Citations

Myocardial inotropic response of rats of different ages to extrasystolic exposures in postinfarction cardiosclerosis

Rebrova

Кондратьева

Афанасьев

et al. 2023

SJCEM

Self Cite

View full text Add to dashboard Cite

The age-dependent features of the inotropic response of isolated strips of rat papillary muscles in postinfarction cardiosclerosis were studied. The rhythmic-inotropic response of the myocardium to extrasystolic effects was assessed in sham-operated (SO) rats and rats with established post-infarction cardiosclerosis (PICS) in the age groups of 4, 12, and 24 months (mon). It was shown that the excitability of the sarcoplasmic membrane of cardiomyocytes increases in 12-mon-old SO animals, and decreases in 24-mon-old animals relative to the group of 4-mon-old SO animals. The ability of the sarcoplasmic reticulum (SPR) of cardiomyocytes to accumulate calcium ions (Ca2+) does not depend on age. Postinfarction myocardial remodeling is accompanied by a decrease in the excitability of the sarcolemma in 4-mon-old animals and an increase in 24-mon-old animals relative to the values in SO rats of the corresponding age. At the same time, the Ca2+-accumulating ability of the SBP decreases in 4- and 12-mon-old animals, remaining unchanged in 24-mon-old animals.

show abstract

Cardiomyocyte mitochondrial respiration and microviscosity in rats of different ages in heart failure

Korepanov

Rebrova

Gorbunov

et al. 2023

Kompleks. probl. serdečno-sosud. zabol.

Self Cite

View full text Add to dashboard Cite

Highlights. Heart failure in older age groups is an urgent medical and social issue. Mitochondrial dysfunction is a key link in the pathogenesis of heart failure. In this study we have demonstrated a decrease in mitochondrial respiratory function in old rats. In this age group, the development of heart failure is accompanied by a further decrease in respiratory control. An increase in the mitochondrial microviscosity in older animals may affect the activity of respiratory chain enzymes in heart failure.Aim. To study the respiratory activity and mitochondrial membrane microviscosity of cardiomyocyte of rats of different ages with heart failure.Methods. The study involved 22 2- and 15-month-old male Wistar rats. The animals were divided into 4 groups: 2 groups of intact animals of both ages (n = 12) and 2 groups of rats with isadrine model of heart failure (HF) (n = 10). HF was modeled by two subcutaneous injections of isoproterenol hydrochloride (170 mg/kg) at interval of 24 hours. Mitochondrial respiratory activity was assessed using respiratory control coefficient. The microviscosity of mitochondrial membranes was evaluated by eximerization coefficients of pyrene-based fluorescent probe in areas of protein-lipid and lipid-lipid contact. Comparative statistical analysis of independent groups was performed using the nonparametric Mann-Whitney test.Results. A decrease in mitochondrial respiratory control in older rats was shown in comparison with young animals. In the HF model, inter-age difference increases, but at the same time, in younger rats, the development of HF is not accompanied by significant changes in mitochondrial respiratory control. An age dependent decrease in the microviscosity of mitochondrial membranes in the area of protein-lipid and lipid-lipid interaction was revealed. In younger rats, the development of HF is characterized by a significant increase in microviscosity in the area of protein-lipid and lipid-lipid contact. In older rats, the development of the pathology is characterized by a significant decrease in microviscosity in the area of protein-lipid interaction.Conclusion. Multidirectional age-related changes in cardiomyocyte mitochondria of rats with heart failure were revealed. It was shown that mitochondria in younger rats retain their functional activity in the HF model unlike older rats.

show abstract

State of the Antioxidant System and the Severity of Lipid-Peroxidation Processes in the Myocardium and Blood Plasma of Rats of Different Ages with Postinfarction Cardiosclerosis

Cited by 3 publications

References 17 publications

Respiration in isolated cardiomyocytes and microviscosity of their membranes in rats of different ages with heart failure

Respiration in isolated cardiomyocytes and microviscosity of their membranes in rats of different ages with heart failure

Myocardial inotropic response of rats of different ages to extrasystolic exposures in postinfarction cardiosclerosis

Cardiomyocyte mitochondrial respiration and microviscosity in rats of different ages in heart failure

Contact Info

Product

Resources

About