State of Stress-Marker Organs in Rats after a Single Exposure to Long-Term Stress and Treatment with Lipopolysaccharide

Алексеева, И. В.; Abramova, A. Yu.; Kozlov, A. Yu.; Коплик, Е. В.; Перцов, А С; Lyadov, D.A.; Nikenina, E. V.; Перцов, С. С.

doi:10.1007/s10517-019-04584-z

Cited by 4 publications

(5 citation statements)

References 9 publications

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“…The described protocol provided 25–35% of susceptible mice and 65–75% of resilient mice. This protocol can be defined as safe and could be conceivably used both in rats and mice [ 91 , 94 , 95 , 96 ].…”

Section: Methodsmentioning

confidence: 99%

Dysregulation of miR-15a-5p, miR-497a-5p and miR-511-5p Is Associated with Modulation of BDNF and FKBP5 in Brain Areas of PTSD-Related Susceptible and Resilient Mice

Maurel

Torrisi

Barbagallo

et al. 2021

IJMS

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Post-traumatic stress disorder (PTSD) is a neuropsychiatric disorder occurring in susceptible individuals following a traumatic event. Understanding the mechanisms subserving trauma susceptibility/resilience is essential to develop new effective treatments. Increasing evidence suggests that non-coding RNAs, such as microRNAs (miRNAs), may play a prominent role in mediating trauma susceptibility/resilience. In this study, we evaluated the transcriptional expression of two key PTSD-related genes (FKBP5 and BDNF) and the relative targeting miRNAs (miR-15a-5p, miR-497a-5p, miR-511-5p, let-7d-5p) in brain areas of PTSD-related susceptible and resilient mice identified through our recently developed mouse model of PTSD (arousal-based individual screening (AIS) model). We observed lower transcript levels of miR-15a-5p, miR-497a-5p, and miR-511a-5p in the hippocampus and hypothalamus of susceptible mice compared to resilient mice, suggesting that the expression of these miRNAs could discriminate the two different phenotypes of stress-exposed mice. These miRNA variations could contribute, individually or synergically, to the inversely correlated transcript levels of FKBP5 and BDNF. Conversely, in the medial prefrontal cortex, downregulation of miR-15a-5p, miR-511-5p, and let-7d-5p was observed both in susceptible and resilient mice, and not accompanied by changes in their mRNA targets. Furthermore, miRNA expression in the different brain areas correlated to stress-induced behavioral scores (arousal score, avoidance-like score, social memory score and PTSD-like score), suggesting a linear connection between miRNA-based epigenetic modulation and stress-induced phenotypes. Pathway analysis of a miRNA network showed a statistically significant enrichment of molecular processes related to PTSD and stress. In conclusion, our results indicate that PTSD susceptibility/resilience might be shaped by brain-area-dependent modulation of miRNAs targeting FKBP5, BDNF, and other stress-related genes.

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Section: Methodsmentioning

confidence: 99%

Dysregulation of miR-15a-5p, miR-497a-5p and miR-511-5p Is Associated with Modulation of BDNF and FKBP5 in Brain Areas of PTSD-Related Susceptible and Resilient Mice

Maurel

Torrisi

Barbagallo

et al. 2021

IJMS

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“…Thus, the stage of the appearance of a significant direct relationship between the weight of the adrenal glands and the indicator of the perceptual component of nociception in rats coincides with the period of development of pronounced multiple changes in these parameters after 24-h immobilization stress [2,8]. It can be assumed that animals with more pronounced adrenal hypertrophy (a typical manifestation of the stress response) are characterized by weakening of the perceptual component of pain sensitivity.…”

Section: Resultsmentioning

confidence: 99%

“…The concentration of cytokines was measured by immune assay on a Bio-Plex device (Bio-Rad). These techniques are described in detail in our previous reports [1,2,8,9].…”

Section: Methodsmentioning

confidence: 99%

“…In our previous studies, it was found that long-term stress exposure in rats is accompanied by involution of the thymus after 3 h, 1 day, and 8 days of the post-stress period, as well as adrenal hypertrophy at the latest stage of observation. These stress-induced changes in the weight of stress marker organs on day 8 were not detected in animals that received an intraperitoneal injection of LPS [2]. It was also found that 24-h immobilization leads, in general, to suppression of pain perception in rats, which is most significant in the late post-stress period (day 8), but does not change the emotional component of nociceptive sensitivity.…”

mentioning

confidence: 88%

“…The consequences of stress are a wide variety of pathological conditions [7]. According to recent reports, stress has a modulating effect on nociceptive sensitivity, the state of stress marker organs, as well as indicators characterizing the intensity of metabolic processes in mammals [2,8,9]. These reactions are largely associated with changes in immune functions under the influence of stress [5].…”

mentioning

confidence: 99%

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Correlation of Physiological Parameters in Rats after Stress Exposure under Conditions of Antigenic Stimulation with Lipopolysaccharide Administration

2021

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We studied correlation dependences between physiological parameters in rats in 3 h, 1 day, and 8 days after administration of LPS (100 μg/kg) at the end of 24-h immobilization stress. In 3 h after LPS administration against the background of stress exposure, significant correlations of metabolic parameters with the relative weight of the adrenal glands and the perceptual component of nociception in rats were revealed. A direct relationship between the concentration of the proinflammatory cytokine TNFα and anti-inflammatory IL-4 was also found in these animals. On the first day after LPS injection, correlations were revealed, predominantly positive, only between the indicators of the cytokine blood profile. In the late post-stress period after antigenic exposure, no correlations between the studied physiological parameters were found. It can be hypothesized that immune modulation through systemic administration of LPS prevents persistent excessive stress of physiological functions at the later stages after stress exposure.

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Dynamics of Metabolic Measures in Rats at Different Stages of the Poststress Period in Conditions of Antigen Exposure by Administration of Lipopolysaccharide

Перцов

Алексеева

Abramova

et al. 2021

Neurosci Behav Physi

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State of Stress-Marker Organs in Rats after a Single Exposure to Long-Term Stress and Treatment with Lipopolysaccharide

Cited by 4 publications

References 9 publications

Dysregulation of miR-15a-5p, miR-497a-5p and miR-511-5p Is Associated with Modulation of BDNF and FKBP5 in Brain Areas of PTSD-Related Susceptible and Resilient Mice

Dysregulation of miR-15a-5p, miR-497a-5p and miR-511-5p Is Associated with Modulation of BDNF and FKBP5 in Brain Areas of PTSD-Related Susceptible and Resilient Mice

Correlation of Physiological Parameters in Rats after Stress Exposure under Conditions of Antigenic Stimulation with Lipopolysaccharide Administration

Dynamics of Metabolic Measures in Rats at Different Stages of the Poststress Period in Conditions of Antigen Exposure by Administration of Lipopolysaccharide

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