State of microbiocenosis of southern chernozem under the no-till system

Melnichuk, T.N.; Egovtseva, A.Yu.; Abdurashytov, S.F.; Abdurashytova, E. R.; Turin, E. N.; Gongalo, A. A.; Zubochenko, A.A.; Pashtetskiy, V S

doi:10.1051/e3sconf/202022404009

Cited by 3 publications

(2 citation statements)

References 26 publications

(27 reference statements)

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“…As a source of microbiota, we chose chernozem, a soil type common in the southern regions of Russia. Cellulolytic capabilities of the chernozem microbiome were reported earlier [ 24 , 25 ]. Our team has already worked with chernozem and demonstrated that it can be a potential source of cellulolytic microorganisms by both traditional microbiology and molecular methods [ 26 , 27 ].…”

Section: Introductionmentioning

confidence: 63%

The Succession of the Cellulolytic Microbial Community from the Soil during Oat Straw Decomposition

Kimeklis

Gladkov

Орлова

et al. 2023

IJMS

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The process of straw decomposition is dynamic and is accompanied by the succession of the microbial decomposing community, which is driven by poorly understood interactions between microorganisms. Soil is a complex ecological niche, and the soil microbiome can serve as a source of potentially active cellulolytic microorganisms. Here, we performed an experiment on the de novo colonization of oat straw by the soil microbial community by placing nylon bags with sterilized oat straw in the pots filled with chernozem soil and incubating them for 6 months. The aim was to investigate the changes in decomposer microbiota during this process using conventional sequencing techniques. The bacterial succession during straw decomposition occurred in three phases: the early phase (first month) was characterized by high microbial activity and low diversity, the middle phase (second to third month) was characterized by low activity and low diversity, and the late phase (fourth to sixth months) was characterized by low activity and high diversity. Analysis of amplicon sequencing data revealed three groups of co-changing phylotypes corresponding to these phases. The early active phase was abundant in the cellulolytic members from Pseudomonadota, Bacteroidota, Bacillota, and Actinobacteriota for bacteria and Ascomycota for fungi, and most of the primary phylotypes were gone by the end of the phase. The second intermediate phase was marked by the set of phylotypes from the same phyla persisting in the community. In the mature community of the late phase, apart from the core phylotypes, non-cellulolytic members from Bdellovibrionota, Myxococcota, Chloroflexota, and Thermoproteota appeared. Full metagenome sequencing of the microbial community from the end of the middle phase confirmed that major bacterial and fungal members of this consortium had genes of glycoside hydrolases (GH) connected to cellulose and chitin degradation. The real-time analysis of the selection of these genes showed that their representation varied between phases, and this occurred under the influence of the host, and not the GH family factor. Our findings demonstrate that soil microbial community may act as an efficient source of cellulolytic microorganisms and that colonization of the cellulolytic substrate occurs in several phases, each characterized by its own taxonomic and functional profile.

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Section: Introductionmentioning

confidence: 63%

The Succession of the Cellulolytic Microbial Community from the Soil during Oat Straw Decomposition

Kimeklis

Gladkov

Орлова

et al. 2023

IJMS

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“…Straw introduction into the soil creates a surplus of nutrients, specifically carbon compounds, which facilitates a new path in the microbiota succession [24]. Earlier we demonstrated that chernozem is a potential source of cellulolytic microorganisms by both traditional microbiology and molecular methods [25,26]. Here we attempted to analyze the process of colonization of the lignocellulosic substrate by the chernozem microbiota and identify its most prominent phases.…”

Section: Discussionmentioning

confidence: 99%

The Succession of the Cellulolytic Microbial Community from the Soil during Oat Straw Decomposition

Kimeklis¹,

Gladkov²,

Orlova³

et al. 2022

Preprint

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The decomposition of straw is a dynamic process, which is reflected in the decomposing microbial consortia, however, this process is poorly understood. Here we performed an experiment on the gradual colonization of oat straw by the soil microbial community. The results showed that soil respiration and bacterial diversity were negatively correlated – while the first decreased, the latter increased. In accordance with the dynamics of diversity and respiration, succession was divided into early, middle, and late decomposition phases. Analysis of 16S rRNA and ITS2 amplicon sequencing data revealed three jointly changing groups of phylotypes, corresponding with distinguished phases of decomposition. The presence of cellulolytic members of Proteobacteria, Bacteroidota, Firmicutes, and Actinobacteriota for bacteria and Ascomycota for fungi had been detected since the early phase, but most of the initial phylotypes were gone by the end of the phase. The second phase marked the functional core with a constant set of other phylotypes from these phyla, persisting in the community. Full metagenome sequencing of the microbial community from the end of the middle phase confirmed that major members of this consortium had GH genes, connected to cellulose and chitin degradation. The appearance of non-cellulolytic members from Bdellovibrio, Myxococcota, Chloroflexi, and Crenarchaeota characterized the last phase of the mature community.

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Taxonomic structure of the southern chernozem under application of biological preparations and different farming systems

Melnichuk

Abdurashytov

Egovtseva

et al. 2021

IOP Conf. Ser.: Earth Environ. Sci.

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The study of the taxonomic structure of the southern chernozem under influence of microbial preparations and various farming systems in the conditions of the southern steppe zone has been carried out. Metagenomic analysis of the microbiome of southern chernozem showed the presence of 174 genera representatives of prokaryotes, among which 17 are dominant, representing more than 1%. Farming systems cause changes in the taxonomic structure of the microbiome in comparison with virgin soil in the steppe zone. The influence of microbial preparations of complex action on the qualitative and quantitative composition of the microbiome of southern chernozem and increasing under no-till farming system has been established. The maximum content of water-soluble organic matter was found in virgin soil, which has higher amount of plant residues, up to 491 mg/kg of soil. Under the influence of farming systems its amount decreased in 4.2-8.5 times. The use of a complex of microbial preparations in the southern chernozem contributed to an increase in the amount of water-soluble fraction of humus depending on the farming system: 1.7 times with the conventional farming system and 3 times with no-till compared to the control without CMP.

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State of microbiocenosis of southern chernozem under the no-till system

Cited by 3 publications

References 26 publications

The Succession of the Cellulolytic Microbial Community from the Soil during Oat Straw Decomposition

The Succession of the Cellulolytic Microbial Community from the Soil during Oat Straw Decomposition

The Succession of the Cellulolytic Microbial Community from the Soil during Oat Straw Decomposition

Taxonomic structure of the southern chernozem under application of biological preparations and different farming systems

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