State-of-art based approaches for anticancer drug-targeting to nucleus

Tiwari, Rahul; Jain, Priyanka; Asati, Saket; Haider, Tanweer; Soni, Vandana; Pandey, Vikas

doi:10.1016/j.jddst.2018.10.011

Cited by 16 publications

(7 citation statements)

References 78 publications

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“…Their moderate tumor uptake may be due to the limited binding rate with the nuclear receptors and rapid metabolism. Effective drug delivery to the nucleus is a challenge because of various physiological barriers, which provide a broader platform for cancer diagnosis and treatment by enhancing nuclear uptake and reducing uptake of normal tissues . In this study, a novel radioiodinated progesterone derivative conjugated with PEGylated phenylboronic acid ( 131 I‐EIPBA) was prepared to enhance the nucleus uptake, and the feasibility of PR targeting was demonstrated in vitro and in vivo.…”

Section: Discussionmentioning

confidence: 99%

Radioiodinated progesterone derivative for progesterone receptor targeting with enhanced nucleus uptake via phenylboronic acid conjugation

Gao

Peng

et al. 2019

Labelled Comp Radiopharmac

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A novel 131I‐radiolabeled probe with aromatic boronate motif (131I‐EIPBA) was designed to target progesterone receptor (PR)–positive breast cancer with enhanced nucleus uptake. Acetylene progesterone was conjugated with pegylated phenylboronic acid via click reaction and radiolabeled with 131I to afford 131I‐EIPBA. Meanwhile, 131I‐EIPB without boronate was prepared as control agent. After determination of the lipophilicity and stability of these tracers, in vitro cell uptake studies and in vivo biodistribution in rats were performed to verify the enhanced nucleus uptake and PR targeting ability of 131I‐EIPBA. 131I‐EIPBA was obtained with moderate radiochemical yield (40.35 ± 3.52%) and high radiochemical purity (>98%). As expected, the high binding affinity (39.58 nM) of 131I‐EIPBA for PR was determined by cell binding assay. The internalization ratio of 131I‐EIPBA was remarkably higher than that of 131I‐EIPB in PR‐positive MCF‐7 cells. Furthermore, the enhanced nucleus uptake of 131I‐EIPBA (0.59 ± 0.02%) was found to be significantly higher than that of 131I‐EIPB (0.13 ± 0.01%) in MCF‐7 cells. A novel 131I‐EIPBA compound was developed for PR targeting with improved cellular nucleus uptake. Furthermore, the introduction of aromatic boronate motif provides a worthwhile strategy for enhancing the nuclear receptor targeting of tracers.

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Section: Discussionmentioning

confidence: 99%

Radioiodinated progesterone derivative for progesterone receptor targeting with enhanced nucleus uptake via phenylboronic acid conjugation

Gao

Peng

et al. 2019

Labelled Comp Radiopharmac

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“…Cell-labeling dyes represent a good study model thanks to their (i) easy monitoring through fluorescence, (ii) high Raman cross sections, (iii) specificity toward given cellular compartments, and (iv) sizes comparable to that of therapeutic drugs. The nuclear labeling dye DAPI was chosen as a model study for therapeutic drugs, which normally target the cellular machinery inside the nucleus. , Concurrently, the membrane labeling DiO was selected as a representative for high-interest therapeutic agents targeting the plasma membrane. − Accordingly, prior to the endoscopy experiments, the nucleus or plasma membrane were labeled with DAPI or DiO, respectively. Investigations through Raman spectroscopy require reference spectra of the compounds under study, to be compared with the spectra obtained via endoscopy.…”

Section: Methodsmentioning

confidence: 99%

Gold-Etched Silver Nanowire Endoscopy: Toward a Widely Accessible Platform for Surface-Enhanced Raman Scattering-Based Analysis in Living Cells

et al. 2021

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Recently, our group introduced the use of silver nanowires (AgNWs) as novel non-invasive endoscopic probes for detecting intracellular Raman signals. This method, although innovative and promising, relies exclusively on the plasmonic waveguiding effect for signal enhancement. It, therefore, requires sophisticated operational tools and protocols, drastically limiting its applicability. Herein, an advanced strategy is offered to significantly enhance the performance of these endoscopic probes, making this approach widely accessible and versatile for cellular studies. By uniformly forming gold structures on the smooth AgNW surface via a galvanic replacement reaction, the density of the light coupling points along the whole probe surface is drastically increased, enabling high surface-enhanced Raman scattering (SERS) efficiency upon solely focusing the excitation light on the gold-etched AgNW. The applicability of these gold-etched AgNW probes for molecular sensing in cells is demonstrated by detecting site-specific and high-resolved SERS spectra of cell compartment-labeling dyes, namely, 4′,6-diamidino-2-phenylindole in the nucleus and 3,3′-dioctadecyloxacarbocyanine on the membrane. The remarkable spectral sensitivity achieved provides essential structural information of the analytes, indicating the overall potential of the proposed approach for cellular studies of drug interactions with biomolecular items.

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“…The nuclear labeling dye DAPI was chosen as a model study for therapeutic drugs, which normally target the cellular machinery inside the nucleus. 28,29 Concurrently, the membrane labeling DiO was selected as a representative for high-interest therapeutic agents targeting the plasma membrane. 30−32 Accordingly, prior to the endoscopy experiments, the nucleus or plasma membrane were labeled with DAPI or DiO, respectively.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

Low-Cytotoxic Gold-Coated Silver Nanoflowers for Intracellular pH Sensing

Zhang

Wen

Watanabe

et al. 2020

ACS Appl. Nano Mater.

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Recently, our group introduced the use of silver nanowires (AgNWs) as novel non-invasive endoscopic probes for detecting intracellular Raman signals. This method, although innovative and promising, relies exclusively on the plasmonic waveguiding effect for signal enhancement. It, therefore, requires sophisticated operational tools and protocols, drastically limiting its applicability. Herein, an advanced strategy is offered to significantly enhance the performance of these endoscopic probes, making this approach widely accessible and versatile for cellular studies. By uniformly forming gold structures on the smooth AgNW surface via a galvanic replacement reaction, the density of the light coupling points along the whole probe surface is drastically increased, enabling high surface-enhanced Raman scattering (SERS) efficiency upon solely focusing the excitation light on the gold-etched AgNW. The applicability of these goldetched AgNW probes for molecular sensing in cells is demonstrated by detecting site-specific and high-resolved SERS spectra of cell compartment-labeling dyes, namely, 4′,6-diamidino-2-phenylindole in the nucleus and 3,3′-dioctadecyloxacarbocyanine on the membrane. The remarkable spectral sensitivity achieved provides essential structural information of the analytes, indicating the overall potential of the proposed approach for cellular studies of drug interactions with biomolecular items.

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State-of-art based approaches for anticancer drug-targeting to nucleus

Cited by 16 publications

References 78 publications

Radioiodinated progesterone derivative for progesterone receptor targeting with enhanced nucleus uptake via phenylboronic acid conjugation

Radioiodinated progesterone derivative for progesterone receptor targeting with enhanced nucleus uptake via phenylboronic acid conjugation

Gold-Etched Silver Nanowire Endoscopy: Toward a Widely Accessible Platform for Surface-Enhanced Raman Scattering-Based Analysis in Living Cells

Low-Cytotoxic Gold-Coated Silver Nanoflowers for Intracellular pH Sensing

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