STAT3-regulated exosomal miR-21 promotes angiogenesis and is involved in neoplastic processes of transformed human bronchial epithelial cells

Liu, Yi; Luo, Fei; Wang, Bairu; Li, Huiqiao; Xu, Yuan; Liu, Xinlu; Shi, Le; Lü, Xiaolin; Xu, Wanlin; Lu, Lu; Qin, Yu; Xiang, Qiaojun; Liu, Qizhan

doi:10.1016/j.canlet.2015.10.011

Cited by 238 publications

(195 citation statements)

References 68 publications

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“…miR-21-5p is a target of NF-κB in regulating cell proliferation in solid tumor 23. However, it has been shown that miR-21-5p is involved in angiogenesis 12, 24, 25. To test whether miR-21-5p is involved in the anti-angiogenic process of Andro, the expression of miR-21-5p was detected.…”

Section: Resultsmentioning

confidence: 99%

Andrographolide Inhibits Angiogenesis by Inhibiting the Mir-21-5p/TIMP3 Signaling Pathway

Dai¹,

Lin²,

Duan³

et al. 2017

Int. J. Biol. Sci.

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Angiogenesis provides nutrients and oxygen to promote tumor growth and affords a channel that facilitates tumor cell entry into the circulation. Andrographolide (Andro) possess anti-tumor activity; however, its direct effect on angiogenesis still needs to be clarified. In this study, our experiments revealed that Andro significantly inhibited vascular growth in chick embryo chorioallantoic membrane (CAM) and yolk sac membrane (YSM) models. Meanwhile, tumor angiogenesis was also suppressed by Andro. Additionally, we found that cell proliferation, migration and tube formation of vascular endothelial cells was inhibited by Andro treatment in vitro. The effect was primarily mediated through inhibition of miR-21-5p expression and further targeting of TIMP3. This work provides evidence that Andro directly inhibits angiogenesis and might be an effective anti-angiogenic therapeutic drug for cancer treatment.

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Section: Resultsmentioning

confidence: 99%

Andrographolide Inhibits Angiogenesis by Inhibiting the Mir-21-5p/TIMP3 Signaling Pathway

Dai¹,

Lin²,

Duan³

et al. 2017

Int. J. Biol. Sci.

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“…In recent years, increasing evidence indicates that EXs function as intercellular messengers to facilitate cancer progression and metastasis [27, 28]. Indeed, tumor-derived EXs have been shown to modify healthy cells by altering their translational profile to promote tumor progression [29].…”

Section: Discussionmentioning

confidence: 99%

Glioma stem cells-derived exosomes promote the angiogenic ability of endothelial cells through miR-21/VEGF signal

et al. 2017

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Glioma stem cells (GSCs) play an important role in glioblastoma prognosis. Exosomes (EXs) mediate cell communication by delivering microRNAs (miRs). Glioblastoma has a high level of miR-21 which could upregulate vascular endothelial growth factor (VEGF) expression. We hypothesized GSC-EXs can promote the angiogenic ability of endothelial cells (ECs) through miR-21/VEGF signal. GSCs were isolated from U-251 cells with stem cell marker CD133. GSCs transfected without or with scramble or miR-21 mimics were used to produce GSC-EXscon, GSC-EXssc and GSC-EXsmiR-21. Human brain ECs were co-cultured with vehicle, GSC-EXscon, GSC-EXssc or GSC-EXsmiR-21 plus VEGF siRNAs (siRNAVEGF). After 24 hours, the angiogenic abilities of ECs were evaluated. The levels of miR-21, VEGF and p-Flk1/VEGFR2 were determined. Results showed: 1) Over 90% of purified GSCs expressed CD133; 2) The levels of miR-21 and VEGF in GSCs and GSC-EXs were up-regulated by miR-21 mimic transfection; 3) Compared to GSC-EXscon or GSC-EXssc, GSC-EXsmiR-21 were more effective in elevating the levels of miR-21 and VEGF, and the ratio of p-Flk1/VEGFR2 in ECs; 4) GSC-EXsmiR-21 were more effective in promoting the angiogenic ability of ECs than GSC-EXscon or GSC-EXssc, which were remarkably reduced by siRNAVEGF pretreatment. In conclusion, GSC-EXs can promote the angiogenic ability of ECs by stimulating miR-21/VEGF/VEGFR2 signal pathway.

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“…Additionally, transformed lung cancer cells have been shown to transfer miR-21 via exosomes to nearby normal bronchial epithelial (HBE) cells. This increases VEGF production and promotes angiogenesis through a STAT3 dependent mechanism [49]. Similarly, hypoxic conditions induce A549 lung cancer cells to release miR-494 containing exosomes to surrounding endothelial cells, which leads to the suppression of PTEN and Akt/eNOS pathway activation, enhancing angiogenesis.…”

Section: Extracellular Micrornas In the Tumour Microenvironmentmentioning

confidence: 99%

Functional Roles for Exosomal MicroRNAs in the Tumour Microenvironment

Bell

Taylor

2017

Computational and Structural Biotechnology Journal

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Extracellular microRNAs are released from cells both passively and actively. The presence of these microRNAs in the tumour microenvironment (TME) can significantly impact on the plasticity of cancer cells leading to the promotion of metastatic and angiogenic processes. These extracellular microRNAs can act not only on other cancer cells, but also cells present in the TME, such as immune cells, endothelial cells, fibroblasts, and others acting to subvert the host immune system and drive tumour progression. In this review we highlight the current understanding of both the mechanisms by which microRNAs are released from tumour cells and the downstream functional effects that extracellular microRNAs have on recipient cells.

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STAT3-regulated exosomal miR-21 promotes angiogenesis and is involved in neoplastic processes of transformed human bronchial epithelial cells

Cited by 238 publications

References 68 publications

Andrographolide Inhibits Angiogenesis by Inhibiting the Mir-21-5p/TIMP3 Signaling Pathway

Andrographolide Inhibits Angiogenesis by Inhibiting the Mir-21-5p/TIMP3 Signaling Pathway

Glioma stem cells-derived exosomes promote the angiogenic ability of endothelial cells through miR-21/VEGF signal

Functional Roles for Exosomal MicroRNAs in the Tumour Microenvironment

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