Starvation-induced posttranscriptional control of rat liver mitochondrial citrate carrier expression

Siculella, Luisa; Sabetta, Simona; Summa, Roberta di; Leo, Monica; Giudetti, Anna Maria; Palmieri, Ferdinando; Gnoni, Gabriele V.

doi:10.1016/s0006-291x(02)02666-9

Cited by 24 publications

(31 citation statements)

References 28 publications

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“…The absence of a consistent difference in the transcriptional rates due to the dietary treatment suggested that Cic gene regulation occurred by a post-transcriptional mechanism. In particular, this study confirmed that the reduced CiC mRNA abundance observed in starved rats could be ascribed to its shorter half-life, suggesting that starvation accelerates the degradation of CiC mRNA (59). The reduced CiC activity in starved animals gradually increased upon refeeding, reaching fed-rat values 6-9 h following refeeding.…”

Section: Starvation and Refeedingsupporting

confidence: 80%

“…These findings suggested that the starvation-induced decrease of CiC activity could be ascribed to modifications of the intrinsic properties of CiC. In fact, a subsequent study showed a considerable reduction ( 40%) of CiC mRNA abundance in starved rats when compared with fed animals (59). The absence of a consistent difference in the transcriptional rates due to the dietary treatment suggested that Cic gene regulation occurred by a post-transcriptional mechanism.…”

Section: Starvation and Refeedingmentioning

confidence: 95%

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The mitochondrial citrate carrier: Metabolic role and regulation of its activity and expression

et al. 2009

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SummaryThe citrate carrier (CiC), a nuclear-encoded protein located in the mitochondrial inner membrane, is a member of the mitochondrial carrier family. CiC plays an important role in hepatic lipogenesis, which is responsible for the efflux of acetyl-CoA from the mitochondria to the cytosol in the form of citrate, the primer for fatty acid and cholesterol synthesis. In addition, CiC is a key component of the isocitrate-oxoglutarate and the citrate-malate shuttles. CiC has been purified from various species and its reconstituted function characterized as well as its cDNA isolated and sequenced. CiC mRNA and/or CiC protein levels are high in liver, pancreas, and kidney, but are low or absent in brain, heart, skeletal muscle, placenta, and lungs. A reduction of CiC activity was found in diabetic, hypothyroid, starved rats, and in rats fed on a polyunsaturated fatty acid (PUFA)-enriched diet. Molecular analysis suggested that the regulation of CiC activity occurs mainly through transcriptional and post-transcriptional mechanisms. This review begins with an assessment of the current understanding of CiC structural and biochemical characteristics, underlying the structure-function relationship. Emphasis will be placed on the molecular basis of the regulation of CiC activity in coordination with fatty acid synthesis.

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Section: Starvation and Refeedingsupporting

confidence: 80%

Section: Starvation and Refeedingmentioning

confidence: 95%

The mitochondrial citrate carrier: Metabolic role and regulation of its activity and expression

et al. 2009

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“…Similar changes were observed in ANIT, carbon tetrachloride, and hydrazineinduced hepatotoxicity (Wu et al, 2005). The reduced organic acid levels suggested that oxidative phosphorylation was inhibited, glycolysis was increased (Siculella et al, 2002;Connor et al, 2004), and that exposure to PCBs and TCDD affected the mitochondrial energy metabolism in liver, although the effect of other factors could not be excluded. The level of glucose in the urine was increased significantly in all treatment groups, which suggested that the rates of gluconeogenesis and glycolysis increased because the lipid metabolism in these animals failed.…”

Section: Discussionmentioning

confidence: 57%

NMR-based metabonomic analysis of the hepatotoxicity induced by combined exposure to PCBs and TCDD in rats

Wang

Sheng

et al. 2010

Toxicology and Applied Pharmacology

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“…TCC has been extensively characterized in liver mitochondria from mammals and fish (for review, see [11]). A parallel decrease of TCC activity and lipogenesis in the liver of starved [12] and n À 6 PUFA-fed rats was reported [13]. The aim of this study was to investigate whether diets with different fatty acid compositions, i.e., rich in either long chain saturated fatty acids (BT) or monounsaturated fatty acids (OO) or n À 3 PUFA (FO) could influence the hepatic TCC expression and, if so, to characterize the molecular mechanism responsible for TCC gene regulation.…”

Section: Introductionmentioning

confidence: 90%

“…The RNA blots were hybridized with rat liver TCC cDNA probe as reported by Siculella et al [12]. For normalization of the hybridization signals, the same membranes were hybridized using a probe encoding part of the human b-actin.…”

Section: Isolation Of Rna and Northern Blot Analysismentioning

confidence: 99%

Different dietary fatty acids have dissimilar effects on activity and gene expression of mitochondrial tricarboxylate carrier in rat liver

et al. 2004

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The tricarboxylate carrier (TCC), an integral protein of the mitochondrial inner membrane, transports mitochondrial acetyl-CoA into the cytosol, where lipogenesis occurs. We investigated in rat liver mitochondria the effect of diets enriched with saturated fatty acids (beef tallow, BT), monounsaturated fatty acids (olive oil, OO) or n À 3 polyunsaturated fatty acids (fish oil, FO), respectively, on the activity and expression of TCC. TCC activity decreased, in parallel with TCC mRNA abundance, only upon FO-feeding. The TCC transcription rate, mRNA turnover and RNA processing indicated that FO administration regulates TCC gene at transcriptional and post-transcriptional steps, whereas BT-and OO-feeding do not seem to affect either TCC activity or gene expression.

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Starvation-induced posttranscriptional control of rat liver mitochondrial citrate carrier expression

Cited by 24 publications

References 28 publications

The mitochondrial citrate carrier: Metabolic role and regulation of its activity and expression

The mitochondrial citrate carrier: Metabolic role and regulation of its activity and expression

NMR-based metabonomic analysis of the hepatotoxicity induced by combined exposure to PCBs and TCDD in rats

Different dietary fatty acids have dissimilar effects on activity and gene expression of mitochondrial tricarboxylate carrier in rat liver

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