Starch and cell wall degrading enzymes from fungal organisms grown on cereal and millet brans

Kavitha, S.; Hegde, Shyamala; Muralikrishna, G.; Varadaraj, M. C.; Rao, E. Rati

doi:10.1007/s00217-004-1101-4

Cited by 4 publications

(2 citation statements)

References 14 publications

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“…Recently, we studied the ability of a strain of A. niger (CFR 1105) to degrade wheat bran (Hegde et al 2006) and also the production of cell wall/starch-degrading enzymes namely, feruloyl esterase, xylanase, 1,3/1,4-b-D glucanase, pullulanase, and amylase (Hegde et al 2006;Kavitha et al 2005). Here, we report further studies on the feruloyl esterase of this organism grown under solid state fermentation (ssf) and submerged fermentation (smf) condition and purification and partial characterization of two isoenzymes (designated as FAE-1 and FAE-2), produced under submerged fermentation conditions.…”

Section: Introductionmentioning

confidence: 99%

Isolation and partial characterization of alkaline feruloyl esterases from Aspergillus niger CFR 1105 grown on wheat bran

Hegde

Muralikrishna

2009

World J Microbiol Biotechnol

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Feruloyl esterases (FAEs) of a strain of Aspergillus niger (CFR 1105) grown in solid state (ssf) and submerged fermentations (smf) using wheat bran both as carbon source and inducer of the enzyme were studied. The feruloyl esterase activity was maximum after 4 days in solid state as well as in submerged fermentations (32.5 and 31.5 U/g dry weight of wheat bran respectively) and the enzyme titers were comparable. The specific activity was maximum on day 2 in ssf (12.8 U/mg protein) and it decreased thereafter, whereas specific activity was maximum on day 3 (11.7 U/mg protein) in smf and it remained constant up to 5 days. Two isoenzymes of feruloyl esterases were isolated and purified to homogeneity by conventional protein purification methods from the day 5 culture filtrate of A. niger grown in smf. On a DEAEcellulose column, two enzyme activity peaks designated as FAE-1 and FAE-2 were eluted with 0.3 and 0.35 M NaCl, respectively. They were monomeric glycoproteins with approximate molecular weights of 50 kDa (FAE-1) and 55 kDa (FAE-2), respectively. FAE-1 showed a temperature optimum of 40°C whereas FAE-2 showed a wider temperature optimum of 40-50°C. FAE-1 and FAE-2 exhibited pH optima of 9 and 6, respectively, and both were stable over a pH range of 6-9. The ability of the enzyme to be active in alkaline pH may be advantageous in biotechnological applications.

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Section: Introductionmentioning

confidence: 99%

Isolation and partial characterization of alkaline feruloyl esterases from Aspergillus niger CFR 1105 grown on wheat bran

Hegde

Muralikrishna

2009

World J Microbiol Biotechnol

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“…produced enzymes which lyses Rhizopus cell walls[21,22]. Likewise, lytic enzyme was produced by Rhizopus strains[23,24]. Apparent form difference of R. peka in the co-culture had little influence on antibiotic activities.…”

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ANTIBIOTIC ACTIVITY IN CO-CULTURE: Influence of <I>Bacillus subtilis</I> on the Antibiotic Activity of <I>Rhizopus peka</I>

2008

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Behavioral Responses of Adult Sitophilus granarius to Individual Cereal Volatiles

2008

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The antennae of Sitophilus granarius (L.) (Coleoptera: Curculionidae) adults detect a wide variety of compounds in the odor blend of various cereal grains (Germinara et al., Tec. Molit., 53:27-34, 2002). In the present study, we looked at the behavioral responses of the granary weevil to 20 of these individual volatiles (aliphatic alcohols, aldehydes, ketones, and aromatics) in a two-choice pitfall olfactometer, using the aggregation pheromone and propionic acid as the attractant and repellent controls, respectively. Five doses, ranging from 1 microg to 1 mg, of each compound were tested. At least one concentration of eight compounds attracted beetles but required doses 1,000- to 5,000-fold higher than the concentration of aggregation pheromone to elicit a response. Three compounds, while attractive at lower concentrations, acted as repellents at higher doses. Twelve compounds were repellent at concentrations similar to the quantity of propionic acid that significantly repelled beetles. The data show that granary weevil adults have the ability to respond behaviorally to a wide range of cereal volatiles and that responses may change as a function of concentration. The results suggest that host finding behavior of weevils will depend on the balance of positive and negative volatile stimuli from grain as the relative concentrations of volatiles may change during storage. An understanding of how the weevils respond to such changes could be useful for the development of effective integrated pest management strategies.

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Starch and cell wall degrading enzymes from fungal organisms grown on cereal and millet brans

Cited by 4 publications

References 14 publications

Isolation and partial characterization of alkaline feruloyl esterases from Aspergillus niger CFR 1105 grown on wheat bran

Isolation and partial characterization of alkaline feruloyl esterases from Aspergillus niger CFR 1105 grown on wheat bran

ANTIBIOTIC ACTIVITY IN CO-CULTURE: Influence of <I>Bacillus subtilis</I> on the Antibiotic Activity of <I>Rhizopus peka</I>

Behavioral Responses of Adult Sitophilus granarius to Individual Cereal Volatiles

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