Starch aided synthesis of giant unilamellar vesicles

Maoyafikuddin, Mohammad; Pundir, Meenakshi; Thaokar, Rochish

doi:10.1016/j.chemphyslip.2019.104834

Cited by 4 publications

(4 citation statements)

References 23 publications

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“…In this study, instead of electroformation, the gel-assisted method was used to prepare vesicles in a low-salt (∼0.3 mM) medium because it generated giant-sized vesicles of a diameter larger than 20 μm with a good yield. For this purpose, a 2 wt % gel was prepared from starch per the protocol described in the literature . Dried lipid stacks on the gel surface were hydrated with a solution containing 0.3 mM NaCl and 0.1 M sucrose for 30 min to obtain surface-adhered vesicles.…”

Section: Methodsmentioning

confidence: 99%

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Effect of the Waveform of the Pulsed Electric Field on the Cylindrical Deformation of Uncharged Giant Unilamellar Vesicles

Maoyafikuddin

Thaokar

2023

Langmuir

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High-speed imaging of giant unilamellar vesicles (GUVs) in recent years has shown significant shape deformation of these vesicles under electroporating direct current (DC) pulsed electric fields, possibly altering the surface distribution of transmembrane potential (TMP) and, thereby, the location and extent of electroporation on the bilayer membrane. The development of TMP, the corresponding shape deformation, and the extent of electroporation depend upon the waveform of the applied electric field. In this work, the deformation of vesicles was carried out under a high-intensity, single cycle of a sinusoidal pulsed electric field (SSPEF) and a square wave pulsed electric field (SWPEF). The cylindrical shape deformations of vesicles were observed for both SSPEF and SWPEF and were dependent upon the ratio of conductivity of the inner medium to the outer medium, α. For α = 1 and α > 1, the vesicles deformed into prolate cylinders as a result of Maxwell stress, whereas they were compressed into oblate cylinders for α < 1. Vesicles subjected to a SSPEF relaxed following either the pore closure dominated t 2 or the efflux and lipid loss dominated, slow t 3 mechanism depending upon the value of α. For α = 1 and α < 1, the relaxation of the vesicles was found to be predominantly dependent upon pore closure. On the other hand, a majority of vesicles gained excess area during poration when α > 1, which can be attributed to a higher TMP and faster charging of the membrane. The predictions of the approximate model for the deformation of vesicles agreed with the experiment, with deviations between the two as a result of the simplicity of the model. Moreover, the degree of deformation of vesicles [measured by the aspect ratio (AR)] and shape deformations of vesicles were found to be dependent upon the pulse width (T P) and amplitude (E 0) of the SSPEF. The specific temporal variation of pore-forming tendencies of SSPEF and SWPEF, with their associated peculiarities, can be judiciously used for controlling electroporation in cells and vesicles.

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Section: Methodsmentioning

confidence: 99%

“…For this purpose, a 2 wt % gel was prepared from starch per the protocol described in the literature. 27 Dried lipid stacks on the gel surface were hydrated with a solution containing 0.3 mM NaCl and 0.1 M sucrose for 30 min to obtain surface-adhered vesicles. Then, the vesicles were harvested from the gel surface.…”

Section: ■ Experimental Sectionmentioning

confidence: 99%

Effect of the Waveform of the Pulsed Electric Field on the Cylindrical Deformation of Uncharged Giant Unilamellar Vesicles

Maoyafikuddin

Thaokar

2023

Langmuir

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“…Thus, the integration of biopolymers (including polysaccharides, proteins, and their derivatives) represents a promising strategy for improving performances of liposomes, making them more protected, stable and consequently applicable [36,39]. Among the biopolymers available to coat liposomes, chitosan is one of the most commonly used [40], besides starch [41], alginate [42] and pectin [43].…”

Section: Liposomesmentioning

confidence: 99%

Lipid-Based Nanostructures for the Delivery of Natural Antimicrobials

2021

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Encapsulation can be a suitable strategy to protect natural antimicrobial substances against some harsh conditions of processing and storage and to provide efficient formulations for antimicrobial delivery. Lipid-based nanostructures, including liposomes, solid lipid nanoparticles (SLNs), and nanostructured lipid nanocarriers (NLCs), are valuable systems for the delivery and controlled release of natural antimicrobial substances. These nanostructures have been used as carriers for bacteriocins and other antimicrobial peptides, antimicrobial enzymes, essential oils, and antimicrobial phytochemicals. Most studies are conducted with liposomes, although the potential of SLNs and NLCs as antimicrobial nanocarriers is not yet fully established. Some studies reveal that lipid-based formulations can be used for co-encapsulation of natural antimicrobials, improving their potential to control microbial pathogens.

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“…As the membrane structure of a real cell is complex, researchers have focused on studying the artificially synthesized giant unilamellar vesicle (GUV) as an alternative to real cells. 25,26 It is becoming a promising material for studying the interaction of NPs with cell membranes because GUVs can be easily synthesized and readily modified to meet specific requirements. There have been numerous studies investigating the interactions between NPs and lipid membranes.…”

Section: Introductionmentioning

confidence: 99%

Increase in anionic Fe₃O₄ nanoparticle-induced membrane poration and vesicle deformation due to membrane potential – an experimental study

Moniruzzaman¹,

Karal²,

Wadud³

et al. 2023

Phys. Chem. Chem. Phys.

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Starch aided synthesis of giant unilamellar vesicles

Cited by 4 publications

References 23 publications

Effect of the Waveform of the Pulsed Electric Field on the Cylindrical Deformation of Uncharged Giant Unilamellar Vesicles

Effect of the Waveform of the Pulsed Electric Field on the Cylindrical Deformation of Uncharged Giant Unilamellar Vesicles

Lipid-Based Nanostructures for the Delivery of Natural Antimicrobials

Increase in anionic Fe₃O₄ nanoparticle-induced membrane poration and vesicle deformation due to membrane potential – an experimental study

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Starch aided synthesis of giant unilamellar vesicles

Cited by 4 publications

References 23 publications

Effect of the Waveform of the Pulsed Electric Field on the Cylindrical Deformation of Uncharged Giant Unilamellar Vesicles

Effect of the Waveform of the Pulsed Electric Field on the Cylindrical Deformation of Uncharged Giant Unilamellar Vesicles

Lipid-Based Nanostructures for the Delivery of Natural Antimicrobials

Increase in anionic Fe3O4 nanoparticle-induced membrane poration and vesicle deformation due to membrane potential – an experimental study

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Increase in anionic Fe₃O₄ nanoparticle-induced membrane poration and vesicle deformation due to membrane potential – an experimental study