Starch accumulation in rice grains subjected to drought during grain filling stage

Prathap,; Ali, Kishwar; Singh, Archana; Vishwakarma, Chandrapal; Krishnan, Veda; Chinnusamy, Viswanathan; Tyagi, Aruna

doi:10.1016/j.plaphy.2019.07.027

Cited by 93 publications

(11 citation statements)

References 65 publications

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“…In legumes, although drought ultimately affects the productivity of grains at all growth stages, the incidence during reproductive and grain development stages results in more substantial grain production loss ( Farooq et al 2017 ). A similar phenomenon was also observed in cereals, such as wheat and rice ( Tripathy et al 2000 ; Farooq et al 2014 ; Prathap et al 2019 ). Drought stress during the reproductive stage can reduce yield dramatically ( Garrity and O’Toole 1994 ; Venuprasad et al 2007 ; Raman et al 2012 ).…”

Section: Introductionsupporting

confidence: 71%

“…Conversely, during the later grain-filling stage, there were no common downregulated DEGs across the two genotypes that directly related to agronomic traits, but there were eight downregulated DEGs that encoded photosystem proteins ( Os01g0773700 , Os03g0747700 , Os03g0778100 , Os07g0148900 , Os07g0673550 , Os08g0119800 , Os09g0475800 and Os09g0481200 ) [see Supporting Information—Table S2 ] . Photosynthesis provides a carbon source for starch accumulation during the grain-filling stage in cereals ( Prathap et al 2019 ). Therefore, a decreased photosynthesis may contribute to poor grain filling, which affects the size and quality of the rice grains.…”

Section: Resultsmentioning

confidence: 99%

“…Drought stress during the reproductive stage can reduce yield dramatically ( Garrity and O’Toole 1994 ; Venuprasad et al 2007 ; Raman et al 2012 ). Likewise, many studies have shown that drought stress also has significant impacts during the grain-filling stage, including decreased photosystem II (PSII) activity ( Pieters and El Souki 2005 ), rapid accumulation of free radical (O 2 ·− ) and hydrogen peroxide (H 2 O 2 ) ( Wang et al 2010 ), fewer sink activities and smaller sink sizes ( Dong et al 2014 ) and early senescence leading to decreased starch in matured grains and the loose packaging of the starch granules ( Prathap et al 2019 ).…”

Section: Introductionmentioning

confidence: 99%

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Transcriptome profiling of two rice genotypes under mild field drought stress during grain-filling stage

Liang

Tabien²,

Tarpley³

et al. 2021

AoB PLANTS

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Drought is one of the most critical abiotic stresses that threaten crop production worldwide. This stress affects the rice crop in all stages of rice development; however, the occurrence during reproductive and grain filling stages has the most impact on grain yield. Although many global transcriptomic studies have been performed during the reproductive stage in rice, very limited information is available for the grain filling stage. Hence, we intend to investigate how the rice plant responds to drought stress during the grain filling stage and how the responses change over time under field conditions. Two rice genotypes were selected for RNA-seq analysis: ‘4610’, previously reported as a moderately tolerant breeding line, and Rondo, an elite indica rice cultivar susceptible to drought conditions. Additionally, ten agronomic traits were evaluated under normal irrigated and drought conditions. Leaf tissues were collected during grain-filling stages at two time points, 14 and 21 days after the drought treatment, from both the drought field and normal irrigated field conditions. Based on agronomic performances, ‘4610’ was less negatively affected than Rondo under mild drought conditions, and expression profiling largely aligned with the phenotypic data. The transcriptomic data indicated that, in general, ‘4610’ had much earlier responses than its counterpart in mitigating the impact of drought stress. Several key genes and gene families related to drought stress or stress-related conditions were found differentially expressed in this study, including transcription factors, drought tolerance genes, and reactive oxygen species (ROS) scavengers. Furthermore, this study identified novel differentially expressed genes (DEGs) without function annotations that may play roles in drought tolerance-related functions. Some of the important DEGs detected in this study can be targeted for future research.

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Section: Introductionsupporting

confidence: 71%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Transcriptome profiling of two rice genotypes under mild field drought stress during grain-filling stage

Liang

Tabien²,

Tarpley³

et al. 2021

AoB PLANTS

View full text Add to dashboard Cite

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“…Enzymes involved in starch accumulation are not only affected by genotype but also by the growing environment of rice (Yang, 2001;Yang J. et al, 2004;Halford et al, 2015;Mayer et al, 2016). Many previous studies found that abiotic factors could affect enzyme activity and subsequently, change total starch, amylose, and amylopectin contents (Pan, 1999;Sun et al, 2018;Cheng et al, 2019;Prathap et al, 2019). Among these factors, enzyme activities are sensitive to environmental factors (like nitrogen fertilizer and temperature), and they can, consequently, affect starch accumulation (Cao et al, 2015;Fu et al, 2019), which is consistent with our results in which the gene expression was suppressed by LT and NPF (Figure 3).…”

Section: Npf and Lt Suppressed Grain Filling Related Characteristicsmentioning

confidence: 99%

Super Rice With High Sink Activities Has Superior Adaptability to Low Filling Stage Temperature

Xu¹,

Yang

Tang³

et al. 2021

Front. Plant Sci.

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To investigate the differential responses of super rice grain filling to low filling stage temperature (LT) and the regulative effect of nitrogen panicle fertilizer (NPF), physiological and molecular experiments were conducted with two super rice varieties (Nanjing 7th: N7 and Nanjing 9108th: N9108) on two different filling stage temperature treatments implemented by applying two sowing dates [Normal filling stage temperature (CK): Sowed on May 30, Tmean = 24.7°C and low filling stage temperature (LT): Sowed on July 1, Tmean = 20.3°C], and two NPF levels (0 and 150 kg N ha−1). In this study, LT, NPF, and simultaneous LT and NPF treatments suppressed the grain filling in all varieties with different levels. Under LT or NPF treatments, the reduction of grain weight, seed setting rate, and filling rate were closely associated with suppressed starch biosynthesis rate in inferior seeds, suggesting that reduced starch biosynthesis rate, expression, and activities of enzymes encoded by related genes, Floury endosperm-4 (FLO4), Starch branching enzyme-I (SBE1), and Starch phosphorylase-L (PHO-l), were responsible for the grain filling reduction. Under LT or NPF treatments, significantly higher grain filling rates and lower variance were found in N9108 compared to that in N7, which were closely related to their higher starch biosynthesis ability, related gene expression, and enzymes activities. One of the probable explanations of the grain filling difference was the variation in the relative amount of key regulative hormones, Abscisic acid (ABA) and 1-aminocyclopropane-1-carboxylic acid (ACC). These results raise a possibility that super rice with higher sink activities has superior adaptability to LT and NPF due to their higher sink activities.

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“…Transformation from starch to monosaccharides is triggered by starch hydrolases (α-amylase and β-amylase) as plants takes a relatively low soluble sugar content providing energy for growth and development [ 7 , 8 ]. Starch accumulation in rice grains is positively correlated with the increase in AGPase and SSs [ 9 ]. At different developmental stages of wheat grains, the starch accumulation rate is significantly positively correlated with GBSS and SBE activities, and the accumulation process of amylopectin, amylose and the starch content after pollination follows a logistic growth equation [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Association among starch storage, metabolism, related genes and growth of Moso bamboo (Phyllostachys heterocycla) shoots

Zhang

Ding

et al. 2021

BMC Plant Biol

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Background Both underground rhizomes/buds and above-ground Moso bamboo (Phyllostachys heterocycla) shoots/culms/branches are connected together into a close inter-connecting system in which nutrients are transported and shared among each organ. However, the starch storage and utilization mechanisms during bamboo shoot growth remain unclear. This study aimed to reveal in which organs starch was stored, how carbohydrates were transformed among each organ, and how the expression of key genes was regulated during bamboo shoot growth and developmental stages which should lay a foundation for developing new theoretical techniques for bamboo cultivation. Results Based on changes of the NSC content, starch metabolism-related enzyme activity and gene expression from S0 to S3, we observed that starch grains were mainly elliptical in shape and proliferated through budding and constriction. Content of both soluble sugar and starch in bamboo shoot peaked at S0, in which the former decreased gradually, and the latter initially decreased and then increased as shoots grew. Starch synthesis-related enzymes (AGPase, GBSS and SBE) and starch hydrolase (α-amylase and β-amylase) activities exhibited the same dynamic change patterns as those of the starch content. From S0 to S3, the activity of starch synthesis-related enzyme and starch amylase in bamboo rhizome was significantly higher than that in bamboo shoot, while the NSC content in rhizomes was obviously lower than that in bamboo shoots. It was revealed by the comparative transcriptome analysis that the expression of starch synthesis-related enzyme-encoding genes were increased at S0, but reduced thereafter, with almost the same dynamic change tendency as the starch content and metabolism-related enzymes, especially during S0 and S1. It was revealed by the gene interaction analysis that AGPase and SBE were core genes for the starch and sucrose metabolism pathway. Conclusions Bamboo shoots were the main organ in which starch was stored, while bamboo rhizome should be mainly functioned as a carbohydrate transportation channel and the second carbohydrate sink. Starch metabolism-related genes were expressed at the transcriptional level during underground growth, but at the post-transcriptional level during above-ground growth. It may be possible to enhance edible bamboo shoot quality for an alternative starch source through genetic engineering.

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Starch accumulation in rice grains subjected to drought during grain filling stage

Cited by 93 publications

References 65 publications

Transcriptome profiling of two rice genotypes under mild field drought stress during grain-filling stage

Transcriptome profiling of two rice genotypes under mild field drought stress during grain-filling stage

Super Rice With High Sink Activities Has Superior Adaptability to Low Filling Stage Temperature

Association among starch storage, metabolism, related genes and growth of Moso bamboo (Phyllostachys heterocycla) shoots

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