Starch accumulation and starch related genes expression in novel inter-specific inbred squash line and their parents during fruit development

Nakkanong, Korakot; Yang, Jiani; Zhang, Ming Fang

doi:10.1016/j.scienta.2011.12.020

Cited by 16 publications

(16 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…And more, there are 109 common upregulated genes during SR development (Additional file 1: Figure S13c). Among these genes, eight genes that belong to starch metabolism pathways [52, 53], including GPT (Glucose-6-phosphate translocator) [53–55], PGM (Phosphoglucomutase), ISA (Isoamylase), SP (starch phosphorylase), DPEP (4-alpha-glucanotransferase) and 3 Beta-amylase genes (Additional file 1: Figure S14). KNOX and MIKC-like (type II MADS-box ) genes [45, 46, 56] were also identified among the 109 genes.…”

Section: Resultsmentioning

confidence: 99%

The wild sweetpotato (Ipomoea trifida) genome provides insights into storage root development

Yang

Xü

et al. 2019

BMC Plant Biol

View full text Add to dashboard Cite

Background Sweetpotato ( Ipomoea batatas (L.) Lam.) is the seventh most important crop in the world and is mainly cultivated for its underground storage root (SR). The genetic studies of this species have been hindered by a lack of high-quality reference sequence due to its complex genome structure. Diploid Ipomoea trifida is the closest relative and putative progenitor of sweetpotato, which is considered a model species for sweetpotato, including genetic, cytological, and physiological analyses. Results Here, we generated the chromosome-scale genome sequence of SR-forming diploid I. trifida var. Y22 with high heterozygosity (2.20%). Although the chromosome-based synteny analysis revealed that the I. trifida shared conserved karyotype with Ipomoea nil after the separation, I. trifida had a much smaller genome than I. nil due to more efficient eliminations of LTR-retrotransposons and lack of species-specific amplification bursts of LTR-RTs. A comparison with four non-SR-forming species showed that the evolution of the beta-amylase gene family may be related to SR formation. We further investigated the relationship of the key gene BMY11 (with identity 47.12% to beta-amylase 1 ) with this important agronomic trait by both gene expression profiling and quantitative trait locus (QTL) mapping. And combining SR morphology and structure, gene expression profiling and qPCR results, we deduced that the products of the activity of BMY11 in splitting starch granules and be recycled to synthesize larger granules, contributing to starch accumulation and SR swelling. Moreover, we found the expression pattern of BMY11 , sporamin proteins and the key genes involved in carbohydrate metabolism and stele lignification were similar to that of sweetpotato during the SR development. Conclusions We constructed the high-quality genome reference of the highly heterozygous I. trifida through a combined approach and this genome enables a better resolution of the genomics feature and genome evolutions of this species. Sweetpotato SR development genes can be identified in I. trifida and these genes perform similar functions and patterns, showed that the diploid I. trifida var. Y22 with typical SR could be considered an ideal model for the studies of sweetpotato SR development. Electronic supplementary material The online version of this article (10.1186/s12870-019-1708-z) contains supplementary material, which is available to authorized users.

show abstract

Section: Resultsmentioning

confidence: 99%

The wild sweetpotato (Ipomoea trifida) genome provides insights into storage root development

Yang

Xü

et al. 2019

BMC Plant Biol

View full text Add to dashboard Cite

show abstract

“…The molecular structure of plant starch consists almost completely of the glucose homopolymers amylopectin and amylase (Tetlow, 2006 ; Jeon et al, 2010 ). The amounts and proportions of amylose and amylopectin in plants vary depending on the type of storage organ, plant species, and cultivar (Gao et al, 1998 ; Sweetlove et al, 1999 ; Vrinten and Nakamura, 2000 ; Nakkanong et al, 2012 ). Total starch and amylase contents were measured in Zhewan 1 and Zhongwan 6 during seed development.…”

Section: Resultsmentioning

confidence: 99%

“…Fully mixed frozen seeds (0.2 g) were prepared for each sample. The amylose contents were evaluated with a dual-wavelength iodine binding colorimetry method as previously described (Nakkanong et al, 2012 ).…”

Section: Methodsmentioning

confidence: 99%

Comparative Transcriptomic Analyses of Vegetable and Grain Pea (Pisum sativum L.) Seed Development

Liu

Zhang

et al. 2015

Front. Plant Sci.

View full text Add to dashboard Cite

Understanding the molecular mechanisms regulating pea seed developmental process is extremely important for pea breeding. In this study, we used high-throughput RNA-Seq and bioinformatics analyses to examine the changes in gene expression during seed development in vegetable pea and grain pea, and compare the gene expression profiles of these two pea types. RNA-Seq generated 18.7 G of raw data, which were then de novo assembled into 77,273 unigenes with a mean length of 930 bp. Our results illustrate that transcriptional control during pea seed development is a highly coordinated process. There were 459 and 801 genes differentially expressed at early and late seed maturation stages between vegetable pea and grain pea, respectively. Soluble sugar and starch metabolism related genes were significantly activated during the development of pea seeds coinciding with the onset of accumulation of sugar and starch in the seeds. A comparative analysis of genes involved in sugar and starch biosynthesis in vegetable pea (high seed soluble sugar and low starch) and grain pea (high seed starch and low soluble sugar) revealed that differential expression of related genes at late development stages results in a negative correlation between soluble sugar and starch biosynthetic flux in vegetable and grain pea seeds. RNA-Seq data was validated by using real-time quantitative RT-PCR analysis for 30 randomly selected genes. To our knowledge, this work represents the first report of seed development transcriptomics in pea. The obtained results provide a foundation to support future efforts to unravel the underlying mechanisms that control the developmental biology of pea seeds, and serve as a valuable resource for improving pea breeding.

show abstract

“…Glucose-1-phosphate adenylyltransferase, an enzyme that is critical for starch synthesis and that catalyzes the first step of starch synthesis ( Nakkanong et al, 2012 ), was proved to be composed of two subunits ( Morell et al, 1987 ). Consistent with LrSUS1 and LrSUS2 genes, the expression levels of genes encoding the large and small subunits of AGPase were significantly decreased in the GA 3 treatment, but in the other treatments (mostly PBZ and ethrel), they were increased at several time points, particularly LrAGPL1 , LrAGPS1 , and LrAGPS2 genes at 3 DAT, and LrAGPL2 and LrAGPS2 genes at 30 DAT ( Figure 7 ).…”

Section: Resultsmentioning

confidence: 99%

Effect of Exogenous Gibberellin, Paclobutrazol, Abscisic Acid, and Ethrel Application on Bulblet Development in Lycoris radiata

Li³

et al. 2021

Front. Plant Sci.

View full text Add to dashboard Cite

Lycoris species have great ornamental and medicinal values; however, their low regeneration efficiency significantly restricts their commercial production. Exogenous hormone application is an effective way to promote bulblet development, but their effect on Lycoris radiata has not been verified to date. In the present study, we examined the effect of different exogenous hormones on bulblet development in L. radiata, and found that gibberellic acid (GA) significantly inhibited, whereas paclobutrazol (PBZ), abscisic acid (ABA), and ethrel promoted bulblet development, especially PBZ, a GA biosynthesis inhibitor. Furthermore, GA reduced endogenous cytokinin (CK) content, as well as the activities of carbohydrate metabolism enzymes, including sucrose synthase (SUS) and glucose-1-phosphate adenylyltransferase (AGPase), by downregulating the expression levels of LrSUS1, LrSUS2, and genes encoding AGPase large and small subunits. This resulted in the decrease in carbohydrate accumulation in the bulblets, thus hindering their development. PBZ had the opposite effect to GA on carbohydrate metabolism; it decreased endogenous GA15 and GA24, thereby promoting bulblet development. ABA promoted endogenous auxin content and the activities of starch synthesis enzymes, especially soluble starch synthase (SSS) and granule-bound SS (GBSS), through the up-regulation of the expression levels of LrSS1, LrSS2, and LrGBSS1 genes, which could also result in the accumulation of carbohydrates in the bulblets and promote their development. In addition, ethrel application partly promoted bulblet development by promoting endogenous CK content. Although the accumulation of carbohydrates and the activity of starch enzymes were increased by ethrel treatment, we hypothesized that the effect of ethrel on regulating carbohydrate metabolism may be indirect. Our results could provide a basis for improving the propagation efficiency of L. radiata for production, as well as propose some directions for future research.

show abstract

Starch accumulation and starch related genes expression in novel inter-specific inbred squash line and their parents during fruit development

Cited by 16 publications

References 45 publications

The wild sweetpotato (Ipomoea trifida) genome provides insights into storage root development

The wild sweetpotato (Ipomoea trifida) genome provides insights into storage root development

Comparative Transcriptomic Analyses of Vegetable and Grain Pea (Pisum sativum L.) Seed Development

Effect of Exogenous Gibberellin, Paclobutrazol, Abscisic Acid, and Ethrel Application on Bulblet Development in Lycoris radiata

Contact Info

Product

Resources

About