Star volume in bone research. A histomorphometric analysis of trabecular bone structure using vertical sections

Vesterby, A.

doi:10.1002/ar.1092350217

Cited by 35 publications

(11 citation statements)

References 43 publications

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“…(9) These calculations demand the presence of a steady state between resorption and formation. (9) Marrow star volume, which reflects the degree of trabecular disintegration caused by perforating resorption, was measured according to the method of Vesterby et al (10) In 17 women from the HRT group and 18 from the pla-cebo group, we succeeded in getting evaluable pre-and post-treatment biopsies. The subsequent analysis is based on these biopsies only.…”

Section: Methodsmentioning

confidence: 99%

Hormone Replacement Therapy Prevents Osteoclastic Hyperactivity: A Histomorphometric Study in Early Postmenopausal Women

Eriksen¹,

Langdahl²,

Vesterby³

et al. 1999

Journal of Bone and Mineral Research

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136

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In a randomized, double blind, clinical prospective trial comprising 35 women treated with either hormone replacement therapy (HRT) (cyclic estradiol/norethisterone acetate) or placebo we performed histomorphometric studies on paired bone biopsies obtained before and after 2 years of treatment. Untreated women developed a progressively more negative balance at individual bone multicellular units (BMUs) (i.e., wall thickness-erosion depth) (2.2 ± 1.7 µm vs. −5.7 ± 1.4 µm; p < 0.01), while women on HRT displayed preservation of bone balance (2.4 ± 2.4 µm vs. 2.5 ± 2.5 µm; NS). No significant differences in wall thickness between the two groups were demonstrable, but the untreated women developed a pronounced increase in erosion depth over 2 years (46.9 ± 1.8 µm vs. 52.0 ± 1.9 µm; p < 0.05), while the HRT group revealed no change (47.8 ± 2.7 µm vs. 44.6 ± 1.7 µm; NS). Furthermore, the placebo group displayed an increased osteoclastic erosion depth (17.8 ± 1.6 µm vs. 25.0 ± 1.7 µm; p < 0.001), compared with unchanged values in the HRT group (20.0 ± 1.6 µm vs. 16.9 ± 1.4 µm/day; NS). While the placebo group revealed a slight increase in volume referent resorption rate (35 ± 8% vs. 38 ± 8%; NS) the HRT group revealed a pronounced decrease (46 ± 8% vs. 28 ± 5%; p < 0.05). No significant changes in marrow star volume (an index of trabecular perforations) were demonstrable in either group. Our results demonstrate that bone remodeling in early postmenopausal women is characterized by progressive osteoclastic hyperactivity, which is reduced by cyclic HRT. This reduction of resorptive activity at the BMU level after HRT seems to precede the reduction in activation frequency demonstrated in previous studies on older postmenopausal women. (J Bone Miner

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Section: Methodsmentioning

confidence: 99%

Hormone Replacement Therapy Prevents Osteoclastic Hyperactivity: A Histomorphometric Study in Early Postmenopausal Women

Eriksen¹,

Langdahl²,

Vesterby³

et al. 1999

Journal of Bone and Mineral Research

Self Cite

136

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“…1 Anterior section, 2 middle section, and 3 posterior section. Directional body axes based on the comparative anatomy approach proposed by Stasek (1963) Aquacult Int (2009) 17:491-506 495 The gonadal biomass (GB) was determined by extrapolation stereology principle described by Versterby (1993): GB = (W vm 9 GA)/100, where GB is the gonadal biomass in g (wet weight), GA is the mean percentage of gonadal area (in % of the mean of the three sections for each individual), and W vm is the visceral mass (gonad ? digestive gland) in g (wet weight).…”

Section: Quantitative Analysesmentioning

confidence: 99%

Gametogenesis, reproductive investment, and spawning behavior of the Pacific giant oyster Crassostrea gigas: evidence of an environment-dependent strategy

et al. 2008

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The progress of gametogenesis was studied in oysters Crassostrea gigas having the same origin (Tremblade), but cultured during 1 year in two distinctive French marine areas, the Baie des Veys and Marennes-Oléron. We assessed seasonal changes in the reproduction cycle on the basis of stereological techniques to estimate reproductive investment and measurement of gonad evolution area by quantitative histology. From a qualitative point of view, both oyster groups presented typical reproductive stages, but showed differential timing, in particular during the sequence of spawning and duration of the re-absorption stage. Oysters in Baie des Veys had a single partial spawning in August and a re-absorption stage that extended until winter. Oysters in Merennes-Oléron had a partial spawning in July and massive release of gametes during August. Spawnings in both the areas were related to maximum temperature (19°C). The quantitative analysis showed, on an annual basis, a higher reproductive investment by oysters from Baie des Veys, 86% against 53% in the other group. Larger gonads, higher gamete production, and more intensive spawning were the characteristics of oysters in Baie des Veys. Recently, the reproduction pattern and investment has been related with summer mortalities; therefore, a quantitative understanding of reproductive processes becomes necessary for C. gigas. Environmental conditions at each site may explain differences in the progress and intensity of gametogenesis. While temperature regulated the time and speed of gametogenesis, results suggest that the intensity was influenced by the quantity of available food but may need further research. However, nutrient recycling from unreleased gametes in the gonads of oysters from Baie des Vey is a factor to be considered in the results of this study.

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“…16,19 The most common method for quantifying the fabric of TB networks is the mean-intercept-length method ͑MIL͒ introduced by Whitehouse in 1974. 20 Other volumetric approaches, such as volume orientation 21 and star volume distribution, 22 have also been used to characterize trabecular morphology. Harrigan and Mann 23 showed that the MIL method applied in three dimensions produces a second-rank tensor that characterizes the structural anisotropy of materials with at least orthotropic symmetry.…”

Section: Introductionmentioning

confidence: 99%

Spatial autocorrelation and mean intercept length analysis of trabecular bone anisotropy applied to in vivo magnetic resonance imaging

et al. 2007

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Osteoporosis is characterized by bone loss and deterioration of the trabecular bone (TB) architecture that leads to impaired overall mechanical strength of the bone. Bone mineral density (BMD) measured by dual-energy x-ray absorptiometry is currently the standard clinical metric assessing bone integrity but it fails to capture the structural changes in the TB. Recent research suggests that structure contributes to bone strength in a manner complementary to BMD. Besides parameters of scale such as the mean TB thickness and mean bone volume fraction, parameters describing the anisotropy of the trabecular architecture play an important role in the characterization of TB since trabeculae are preferentially oriented along the direction of local loading. Therefore, the degree of structural anisotropy is of pivotal importance to the bone's mechanical competence. The most common method for measuring structural anisotropy of TB is the mean-intercept length (MIL). In this work we present a method, based on the three-dimensional spatial autocorrelation function (ACF), for mapping of the full structural anisotropy ellipsoid of both TB thickness and spacing and we examine its performance as compared to that of MIL. Not only is the ACF method faster by several orders of magnitude, it is also considerably more robust to noise. Further, it is applicable at lower spatial resolution and is relatively insensitive to image shading. The chief reason for ACF's superior performance is that it does not require binarization, which is difficult to achieve in the limited spatial regime of in vivo magnetic resonance imaging. MIL and ACF have been applied to high-resolution magnetic resonances images of the tibia in a group of ten healthy postmenopausal women by comparing the structural anisotropy and principal direction of the computed fabric tensor for each method. While there is fair agreement between the two methods, ACF analysis yielded greater anisotropy than MIL for both TB thickness and spacing. There was good agreement between the two techniques as far as the eigenvectors of the fabric ellipsoids were concerned, which parallel the bone's macroscopic axis.

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Star volume in bone research. A histomorphometric analysis of trabecular bone structure using vertical sections

Cited by 35 publications

References 43 publications

Hormone Replacement Therapy Prevents Osteoclastic Hyperactivity: A Histomorphometric Study in Early Postmenopausal Women

Hormone Replacement Therapy Prevents Osteoclastic Hyperactivity: A Histomorphometric Study in Early Postmenopausal Women

Gametogenesis, reproductive investment, and spawning behavior of the Pacific giant oyster Crassostrea gigas: evidence of an environment-dependent strategy

Spatial autocorrelation and mean intercept length analysis of trabecular bone anisotropy applied to in vivo magnetic resonance imaging

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