Star polymer-based nanolayers with immobilized complexes of polycationic stars and DNA for deposition gene delivery and recovery of intact transfected cells

Mendrek, Barbara; Fus-Kujawa, Agnieszka; Teper, Paulina; Botor, Malwina; Kubacki, Jerzy; Sieroń, Aleksander; Kowalczuk, Agnieszka

doi:10.1016/j.ijpharm.2020.119823

Cited by 8 publications

(8 citation statements)

References 56 publications

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“…When the polymer solution was heated above the cloud point temperature (T CP ), given as 50% transmittance at the heating cycle, and then cooled to room temperature, practically no hysteresis of transition was observed (Figure 3b). An important factor influencing the biomedical applicability of polymers is the presence of various salts in the solution and their effect on the thermosensitivity of the polymers [36,37]. Figure 3c shows the cloud points of the copolymer recorded in the presence of increasing amounts of sodium chloride.…”

Section: Properties In the Aqueous Solutionmentioning

confidence: 99%

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Alternative to Poly(2-isopropyl-2-oxazoline) with a Reduced Ability to Crystallize and Physiological LCST

Wałach

Klama-Baryła

Sitkowska

et al. 2021

IJMS

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In this work, we sought to examine whether the presence of alkyl substituents randomly distributed within the main chain of a 2-isopropyl-2-oxazoline-based copolymer will decrease its ability to crystallize when compared to its homopolymer. At the same time, we aimed to ensure an appropriate hydrophilic/lipophilic balance in the copolymer and maintain the phase transition in the vicinity of the human body temperature. For this reason, copolymers of 2-ethyl-4-methyl-2-oxazoline and 2-isopropyl-2-oxazoline were synthesized. The thermoresponsive behavior of the copolymers in water, the influence of salt on the cloud point, the presence of hysteresis of the phase transition and the crystallization ability in a water solution under long-term heating conditions were studied by turbidimetry. The ability of the copolymers to crystallize in the solid state, and their thermal properties, were analyzed by differential scanning calorimetry and X-ray diffractometry. A cytotoxicity assay was used to estimate the viability of human fibroblasts in the presence of the obtained polymers. The results allowed us to demonstrate a nontoxic alternative to poly(2-isopropyl-2-oxazoline) (PiPrOx) with a physiological phase transition temperature (LCST) and a greatly reduced tendency to crystallize. The synthesis of 2-oxazoline polymers with such well-defined properties is important for future biomedical applications.

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Section: Properties In the Aqueous Solutionmentioning

confidence: 99%

“…In conclusion, when short-term heating conditions are applied, P(EtMetOx 10 -iPrOx 90 ) exhibits a thermoresponsive behavior comparable to that of PiPrOx. thermosensitivity of the polymers [36,37]. Figure 3c shows the cloud points of the copolymer recorded in the presence of increasing amounts of sodium chloride.…”

Section: Properties In the Aqueous Solutionmentioning

confidence: 99%

Alternative to Poly(2-isopropyl-2-oxazoline) with a Reduced Ability to Crystallize and Physiological LCST

Wałach

Klama-Baryła

Sitkowska

et al. 2021

IJMS

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“…Layers of star (co)polymers ( Figure 8 b) of OEGMA with DEGMA and glycidyl methacrylate (GMA) [ 65 , 72 ], DMAEMA [ 108 ] and hyperbranched polyglycidol ( Figure 8 c) [ 109 ] were prepared. Star (co)polymers were linked to the support by the “grafting to” method [ 65 , 72 , 108 ], while hyperbranched polyglycidol was linked by “grafting from” [ 109 ]. A schematic description of the properties of the obtained layers is provided in Table 3 .…”

Section: Polymer Nano- and Microstructurementioning

confidence: 99%

“…The layers made of poly(oligo(ethylene glycol) methacrylate) stars were thermoresponsive [ 65 , 72 ]. POEGMA star layers were used for culturing fibroblasts [ 65 ] or HT-1080 cells [ 72 ], and their noninvasive detachment was controlled only by changing the temperature [ 65 , 72 ]. The detachment of the fibroblast sheet from star POEGMA layers occurred faster than that from linear P(TEGMA-EE) brushes [ 86 ].…”

Section: Polymer Nano- and Microstructurementioning

confidence: 99%

“…The detachment of the fibroblast sheet from star POEGMA layers occurred faster than that from linear P(TEGMA-EE) brushes [ 86 ]. Thermoresponsive star POEGMA layers were also used for the deposition of DNA polymer carriers, which opened a route to the efficient delivery of nucleic acids into cells [ 72 ]. At an environmental temperature above the T CP of the nanolayer and simultaneously the DNA polymer carrier, polyplex deposition was performed.…”

Section: Polymer Nano- and Microstructurementioning

confidence: 99%

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The Role of Polymer Structure in Formation of Various Nano- and Microstructural Materials: 30 Years of Research in the Laboratory of Nano- and Microstructural Materials at the Centre of Polymer and Carbon Materials PAS

et al. 2021

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The review summarizes the research carried out in the Laboratory of Nano- and Microstructural Materials at the Centre of Polymer and Carbon Materials, Polish Academy of Sciences (CMPW PAS). Studies carried out for many years under the guidance of Professor Andrzej Dworak led to the development and exploration of the mechanisms of oxirane and cyclic imine polymerization and controlled radical polymerization of methacrylate monomers. Based on that knowledge, within the last three decades, macromolecules with the desired composition, molar mass and topology were obtained and investigated. The ability to control the structure of the synthesized polymers turned out to be important, as it provided a way to tailor the physiochemical properties of the materials to their specific uses. Many linear polymers and copolymers as well as macromolecules with branched, star, dendritic and hyperbranched architectures were synthesized. Thanks to the applied controlled polymerization techniques, it was possible to obtain hydrophilic, hydrophobic, amphiphilic and stimulus-sensitive polymers. These tailor-made polymers with controlled properties were used for the construction of various types of materials, primarily on the micro- and nanoscales, with a wide range of possible applications, mainly in biomedicine. The diverse topology of polymers, and thus their properties, made it possible to obtain various types of polymeric nanostructures and use them as nanocarriers by encapsulation of biologically active substances. Additionally, polymer layers were obtained with features useful in medicine, particularly regenerative medicine and tissue engineering.

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Poly(oligoethylene glycol methacrylate) Star‐Shaped Copolymers with Hydroxypropyl Methacrylate Cores

Sentoukas

Foryś

Marcinkowski

et al. 2022

Macro Chemistry & Physics

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Star-shaped copolymers with crosslinked hyperbranched cores, varying in both number and length of the arms, are prepared by the reversible addition-fragmentation chain transfer (RAFT) polymerization technique. Star synthesis is conducted via a simple, "core-first" method. Hydroxypropyl methacrylate monomer is used for the formation of the hyperbranched/crosslinked core, with ethylene glycol dimethacrylate used as a bifunctional monomer in the role of the cross-linker. Two different cross-linker/chain transfer agent ratios are chosen to manipulate the number of the arms in the final star nanostructure. Oligoethylene glycol methacrylate (OEGMA 500 ) is used as a biocompatible and hydrophilic component for the creation and extension of the arms. Arm length is controlled by the amount of OEGMA monomer used in the second polymerization step. Molecular characterization of the four synthesized stars is achieved via size exclusion chromatography and nuclear magnetic resonance, which provides information regarding the molar mass, dispersity, and composition of each star-shaped copolymer. Dynamic light scattering (DLS) studies of copolymers in N,N-dimethyl formamide solutions provide sizes of individual copolymer molecules in the size range of 8-20 nm. Aqueous dispersions of copolymers are investigated by DLS, cryogenic transmission electron microscopy, and atomic force microscopy, which show the formation of spherical aggregates with diameters of 60-350 nm, dependent on the arm number and length of star copolymers. The obtained sizes and size distributions, along with the hydroxyl-group-bearing cores, indicate that the star copolymer can be used as potential nanocarriers for drugs or bioimaging agents.

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Star polymer-based nanolayers with immobilized complexes of polycationic stars and DNA for deposition gene delivery and recovery of intact transfected cells

Cited by 8 publications

References 56 publications

Alternative to Poly(2-isopropyl-2-oxazoline) with a Reduced Ability to Crystallize and Physiological LCST

Alternative to Poly(2-isopropyl-2-oxazoline) with a Reduced Ability to Crystallize and Physiological LCST

The Role of Polymer Structure in Formation of Various Nano- and Microstructural Materials: 30 Years of Research in the Laboratory of Nano- and Microstructural Materials at the Centre of Polymer and Carbon Materials PAS

Poly(oligoethylene glycol methacrylate) Star‐Shaped Copolymers with Hydroxypropyl Methacrylate Cores

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