Staphylococcus succinus 14BME20 Prevents Allergic Airway Inflammation by Induction of Regulatory T Cells via Interleukin-10

Abstract

Asthma is a common chronic inflammatory disease, which is characterized by airway hyperresponsiveness (AHR), high serum levels of immunoglobulin (Ig)E, and recruitment of various inflammatory cells such as eosinophils and lymphocytes. Korean traditional fermented foods have been reported to exert beneficial effects against allergic diseases such as asthma and atopic dermatitis. In this study, we investigated whether Staphylococcus succinus strain 14BME20 (14BME20) isolated from doenjang, a traditional high-salt-fermented soybean food of Korea, exerts suppressive effects on allergic airway inflammation in a murine model. Mice were orally administered with 14BME20, then sensitized and challenged with ovalbumin as an allergen. Administration of the 14BME20 significantly suppressed AHR and influx of inflammatory cells into the lungs and reduced serum IgE levels. Moreover, the proportion of T helper type 2 (Th2) cells and the production of Th2 cytokines were decreased in 14BME20-treated mice, whereas dendritic cells (DCs) with tolerogenic characteristics were increased. In contrast, oral administration of 14BME20 increased the proportion of CD4 + CD25 + Foxp3 + regulatory T (Treg) cells and the level of interleukin (IL)-10 in 14BME20-treated mice. Furthermore, 14BME20 induced maturation of tolerogenic DCs, and 14BME20-treated DCs increased Treg cell population in a co-culture system of DCs and CD4 + T cells. The addition of a neutralizing anti-IL-10 mAb to the culture of cells that had been treated with 14BME20 decreased the enhanced Treg cell population, thereby indicating that 14BME20-treated DCs increase Treg cell population via DC-derived IL-10. These results demonstrate that oral administration of 14BME20 suppresses airway inflammation by enhancing Treg responses and suggest that the 14BME20 isolated from doenjang may be a therapeutic agent for allergic asthma.

A New Formulation of Probiotics Attenuates Calcipotriol-Induced Dermatitis by Inducing Regulatory Dendritic Cells

“…Probiotics have been reported to mitigate allergic diseases through the induction of Tregs, inhibition of Th2 immune responses, and promotion of intestinal DCregs expansion ( 2 , 41 , 42 ). Although innate immune responses play an important role in the MC903-induced AD-like dermatitis, in which TSLP and IL-33 produced by keratinocytes initiate the skin inflammation, adaptive immune cells are also required in the inflammation.…”

Modulatory Effects of Probiotics During Pathogenic Infections With Emphasis on Immune Regulation

“…Staphylococcus succinus 14BME20 has also shown antiallergic potential; it significantly decreases the influx of inflammatory cells into the lungs, suppresses airway hyperresponsiveness, and reduces the serum IgE and Th2 cells cytokines production in an ovalbumin mice model (308). Clostridium butyricum CGMCC0313 increases forkhead box P3 (FOXP3) Treg cells, TGF-β, and IL10, inverts the imbalance between Th1/Th2 and Th17/Treg cells, and reduces β-lactoglobulin-mediated intestinal anaphylaxis, thereby contributing to the reduction of the risk of allergy in mice (309).…”

Epiphytic Microbial Community and Post-Harvest Characteristics of Strawberry Fruits as Affected by Plant Nutritional Regime with Silicon

“…As per B. breve, also this beneficial species was decreased by the Si biofortification (Figure 3). Among potential probiotics, two species were detected and found instead to increase with Si treatment: S. salivarius [52] and S. succinus [53].…”