Staphylococcus petrasii sp. nov. including S. petrasii subsp. petrasii subsp. nov. and S. petrasii subsp. croceilyticus subsp. nov., isolated from human clinical specimens and human ear infections

Pantůček, Roman; Švec, Pavel; Dajcs, Joseph J.; Machová, Ivana; Černohlávková, Jitka; Šedo, Ondřej; Gelbíčová, Tereza; Mašlaňová, Ivana; Doškař, Jiřı́; Zdráhal, Zbyněk; Růžičková, Vladislava; Sedláček, Ivo

doi:10.1016/j.syapm.2012.11.004

Cited by 36 publications

(26 citation statements)

References 19 publications

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“…These bacteria are ubiquitous and human common commensals isolated mainly from the skin and mucous membranes of mammals. However, they have been recently recognized as opportunistic pathogens in several human infections (Pantůček and others ) as also demonstrated for St. saprophyticus , which was found to be involved in urinary tract infection mainly in young women (Raz and others ). Other taxa were also found, such as Leuc.…”

Section: Resultsmentioning

confidence: 94%

“…In fact, an analysis of the indoor air showed that some species belonging to the Staphylococcus genera were widespread, including Staphylococcus saprophyticus and Staphylococcus petrasii / Staphylococcus jettensis/Staphylococcus hominis/Staphylococcus haemolyticus . St. petrasii , St. jettensis , St. hominis , and St. haemolyticus are coagulase‐negative staphylococci, which are phylogenetically related and phenotypically similar (Pantůček and others ). These bacteria are ubiquitous and human common commensals isolated mainly from the skin and mucous membranes of mammals.…”

Section: Resultsmentioning

confidence: 99%

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The Occurrence of Beer Spoilage Lactic Acid Bacteria in Craft Beer Production

Garofalo

Osimani

Milanović

et al. 2015

Journal of Food Science

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Beer is one of the world's most ancient and widely consumed fermented alcoholic beverages produced with water, malted cereal grains (generally barley and wheat), hops, and yeast. Beer is considered an unfavorable substrate of growth for many microorganisms, however, there are a limited number of bacteria and yeasts, which are capable of growth and may spoil beer especially if it is not pasteurized or sterile-filtered as craft beer. The aim of this research study was to track beer spoilage lactic acid bacteria (LAB) inside a brewery and during the craft beer production process. To that end, indoor air and work surface samples, collected in the brewery under study, together with commercial active dry yeasts, exhausted yeasts, yeast pellet (obtained after mature beer centrifugation), and spoiled beers were analyzed through culture-dependent methods and PCR-DGGE in order to identify the contaminant LAB species and the source of contamination. Lactobacillus brevis was detected in a spoiled beer and in a commercial active dry yeast. Other LAB species and bacteria ascribed to Staphylococcus sp., Enterobaceriaceae, and Acetobacter sp. were found in the brewery. In conclusion, the PCR-DGGE technique coupled with the culture-dependent method was found to be a useful tool for identifying the beer spoilage bacteria and the source of contamination. The analyses carried out on raw materials, by-products, final products, and the brewery were useful for implementing a sanitization plan to be adopted in the production plant.

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Section: Resultsmentioning

confidence: 94%

Section: Resultsmentioning

confidence: 99%

The Occurrence of Beer Spoilage Lactic Acid Bacteria in Craft Beer Production

Garofalo

Osimani

Milanović

et al. 2015

Journal of Food Science

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“…Phenotypic characterization. Extensive phenotypic characterization using the commercial kits API 50CH, API ID 32 Staph, and API ZYM (bioMérieux), phenotypic fingerprinting using the Biolog system with the identification test panel GEN III MicroPlate (Biolog), and conventional biochemical, physiological, and growth tests relevant for the genus Staphylococcus were done as described previously (50,51). All phenotypic data presented were from two replicates using commercial kits and three replicates using conventional tests.…”

Section: Methodsmentioning

confidence: 99%

Staphylococcus edaphicus sp. nov., Isolated in Antarctica, Harbors the mecC Gene and Genomic Islands with a Suspected Role in Adaptation to Extreme Environments

Pantůček

Sedláček

Indráková

et al. 2018

Appl Environ Microbiol

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Two Gram-stain-positive, coagulase-negative staphylococcal strains were isolated from abiotic sources, stone fragments and sandy soil in James Ross Island, Antarctica. Here we describe properties of novel species of the genus that has near identical 16S rRNA gene sequence to However, compared to and the next closest relatives, the new species demonstrates considerable phylogenetic distance at whole genome level, average nucleotide identity<85 %, and inferred DNA-DNA hybridization <30%. It forms a separate branch in phylogenetic clade confirmed by multilocus sequence analysis of six housekeeping genes, ,, ,, and MALDI-TOF MS and key biochemical characteristics allowed these bacteria to be distinguished from their nearest phylogenetic neighbours. In contrast to subsp. , strains were pyrrolidonyl arylamidase- and β-glucuronidase-positive, β -galactosidase-negative, nitrate was reduced and acid produced aerobically from d-mannose. Whole genome sequencing of 2.75 Mb large chromosome revealed the presence of a number of mobile genetic elements including the 27-kb-long pseudo staphylococcus cassette chromosome (ψSCC) harboring gene, two composite phage-inducible chromosomal islands probably essential to adaptation to extreme environment, and one complete and one defective prophage. Both strains are resistant to penicillin G, ampicillin, ceftazidin, methicillin, cefoxitin, and fosfomycin. We hypothesize that antibiotic resistance might represent an evolutionary advantage against beta-lactams producers, which are common in a polar environment. Based on these results, a novel species of the genus is described and named sp. nov. The type strain is P5085 (=CCM 8730 = DSM 104441). The description of sp. nov. enables the comparison of multidrug-resistant staphylococci from human and veterinary sources evolved in the globalized world to their geographically distant relative from the extreme Antarctic environment. Although this new species was not exposed to the pressure of antibiotic treatment in human or veterinary practice, mobile genetic elements carrying antimicrobial resistance genes were found in the genome. The presented genomic characteristics elucidate the evolutionary relationships in the genus with a special focus on antimicrobial resistance, pathogenicity and survival traits. Genes encoded on mobile genetic elements were arranged in unique combinations but retained conserved locations for the integration of mobile genetic elements. These findings point to enormous plasticity of the staphylococcal pangenome shaped by horizontal gene transfer. Thus can act not only as a reservoir of antibiotic resistance in a natural environment, but also as a mediator for the spread and evolution of resistance genes.

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“…petrasii and Staphylococcus petrasii subsp. croceilyticus were recognized (Pantu˚ček et al , 2013). Recently, another coagulase-negative species Staphylococcus jettensis (De Bel et al , 2013) was revealed to be a member of the species S. petrasii and was reclassified as Staphylococcus petrasii subsp.…”

mentioning

confidence: 99%

“…Subsequently, ten clinical isolates were examined: three isolates of S. petrasii subsp. petrasii (Pantu˚ček et al , 2013), two isolates each of S. petrasii subsp. croceilyticus (Pantu˚ček et al , 2013) and S. petrasii subsp.…”

mentioning

confidence: 99%

Staphylococcus petrasii subsp. pragensis subsp. nov., occurring in human clinical material

Švec

Bel

Sedláček

et al. 2015

International Journal of Systematic and Evolutionary Microbiology

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Seven coagulase-negative, oxidase-negative and novobiocin-susceptible staphylococci assigned tentatively as Staphylococcus petrasii were investigated in this study in order to elucidate their taxonomic position. All strains were initially shown to form a genetically homogeneous group separated from remaining species of the genus Staphylococcus by using a repetitive sequence-based PCR fingerprinting with the (GTG)(5) primer. Phylogenetic analysis based on 16S rRNA gene, hsp60, rpoB, dnaJ, gap and tuf sequences showed that the group is closely related to Staphylococcus petrasii but separated from the three hitherto known subspecies, S. petrasii subsp. petrasii, S. petrasii subsp. croceilyticus and S. petrasii subsp. jettensis. Further investigation using automated ribotyping, MALDI-TOF mass spectrometry, fatty acid methyl ester analysis, DNA-DNA hybridization and extensive biotyping confirmed that the analysed group represents a novel subspecies within S. petrasii, for which the name Staphylococcus petrasii subsp. pragensis subsp. nov. is proposed. The type strain is NRL/St 12/356(T) (=CCM 8529(T)=LMG 28327(T))

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Staphylococcus petrasii sp. nov. including S. petrasii subsp. petrasii subsp. nov. and S. petrasii subsp. croceilyticus subsp. nov., isolated from human clinical specimens and human ear infections

Cited by 36 publications

References 19 publications

The Occurrence of Beer Spoilage Lactic Acid Bacteria in Craft Beer Production

The Occurrence of Beer Spoilage Lactic Acid Bacteria in Craft Beer Production

Staphylococcus edaphicus sp. nov., Isolated in Antarctica, Harbors the mecC Gene and Genomic Islands with a Suspected Role in Adaptation to Extreme Environments

Staphylococcus petrasii subsp. pragensis subsp. nov., occurring in human clinical material

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