Staphylococcus lugdunensis sp. nov. and Staphylococcus schleiferi sp. nov., Two Species from Human Clinical Specimens

Freney, J.; Brun, Y.; Bes, Michèle; Meugnier, H.; Grimont, Francine; Grimont, Patrick A. D.; Nervi, Chantal; Fleurette, J

doi:10.1099/00207713-38-2-168

Cited by 360 publications

(271 citation statements)

References 28 publications

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“…Thirteen reference strains were used: Staphylococcus caprae (DSM 20608) (Kawamura et al, 1998); Staphylococcus cohnii (DSM 20260), Staphylococcus haemolyticus (DSM 20263), Staphylococcus saprophyticus (DSM 20229) and Staphylococcus xylosus (DSM 20267) ; Staphylococcus hominis (DSM 20328), Staphylococcus simulans (DSM 20322) and Staphylococcus warneri (DSM 20316) ; Staphylococcus epidermidis (DSM 1798) (Winslow, 1908); Staphylococcus hyicus (DSM 20459) (Schleifer & Kocur, 1973); Staphylococcus intermedius (DSM 20373) (Hajek, 1976); and Staphylococcus lugdunensis (DSM 4804) and Staphylococcus schleiferi (DSM 4807) (Freney et al, 1988).…”

Section: Methodsmentioning

confidence: 99%

Evaluation of species-specific score cut-off values for various Staphylococcus species using a MALDI Biotyper-based identification

Richter

Hollstein

Woloszyn

et al. 2012

Journal of Medical Microbiology

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Although previous studies investigating the MALDI Biotyper database (matrix-assisted laser desorption/ionization time-of-flight mass spectrometry-based identification) have proven its high accuracy for bacterial identification, the studies differed in sample preparation, number of replicates, quantity of shots and target types used. In particular, the score cut-off values of special importance for reliable species identification varied. The aim of the present study was to identify species-specific differences in the mean score values for staphylococci. Cut-off values recommended by the manufacturer were adapted using the 20th percentile to rule out unknown score-modifying factors, even though the specificity was high and the lowest cut-off values would also yield an accurate result. Whilst correct species diagnosis was obtained in 97.32 % of samples (1382/1420), only 220 of all duplicates (15.49 %) revealed a score of ¢2.3, whilst 968 (68.17 %) had a score between 2.0 and 2.299, and 194 (13.66 %) had a score of ,2.0. Ten of 21 species had a calculated 20th percentile of ,2.0 and one species of ,1.7. In conclusion, the use of species-specific cut-off values improves the relative sensitivity of species identification in staphylococci.

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Section: Methodsmentioning

confidence: 99%

Evaluation of species-specific score cut-off values for various Staphylococcus species using a MALDI Biotyper-based identification

Richter

Hollstein

Woloszyn

et al. 2012

Journal of Medical Microbiology

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“…This study shows a defect in the bactericidal activity of glycopeptides against CoNS of the S. lugdunensis species. Since its description in 1988 (8), this species, shown to be part of the normal skin flora, has been described as being one of the most pathogenic CoNS (9). Indeed, S. lugdunensis infections resemble S. aureus infections (9) in terms of virulence, tissue destruction, and clinical course, particularly for endocarditis (22).…”

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confidence: 99%

Tolerance to the Glycopeptides Vancomycin and Teicoplanin in Coagulase-Negative Staphylococci

Bourgeois

Pestel-Caron

Lemeland

et al. 2007

Antimicrob Agents Chemother

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Tolerance to vancomycin and teicoplanin in 90 clinical isolates of coagulase-negative staphylococci (CoNS) was investigated by time-kill curve methodology. Only six strains, belonging to the Staphylococcus lugdunensis species, exhibited tolerance. The seven other S. lugdunensis strains tested displayed weak susceptibility to the bactericidal activity of glycopeptides compared to the other CoNS. These phenomena are of concern, since S. lugdunensis is recognized as one of the most pathogenic CoNS.Coagulase-negative staphylococci (CoNS) are involved in infections that require bactericidal treatment, such as indwelling foreign body-related infections, endocarditis, and meningitis (4, 10). As CoNS become more resistant to beta-lactams (2), glycopeptides are often considered to be antibiotics of last resort (12). Some investigators, however, have reported glycopeptide tolerance for sporadic CoNS (16,23). Antibiotic tolerance describes a particular "type of resistance" in bacteria capable of surviving, but not growing, in the presence of a normally lethal dose of a given bactericidal antibiotic (20, 21). As early screenings for glycopeptide tolerance in CoNS have been performed by the controversial minimal bactericidal concentration (MBC)/MIC determinations (1,14,19,21), the present study was designed to examine vancomycin and teicoplanin tolerance in a collection of clinically significant CoNS by using the killing curve method, which is considered to be the most reliable method according to the Clinical and Laboratory Standards Institute (CLSI) (formerly NCCLS) (14).An initial set of 79 clinically significant isolates of CoNS from 79 individual patients attending the Rouen University Hospital between January 1999 and April 2001 was studied. Strains were identified to the species level with the ID32Staph system (bioMérieux, Marcy l'Etoile, France) and by a gap gene PCR-restriction fragment length polymorphism assay (24). This set reflected the current epidemiology of CoNS (11), with Staphylococcus epidermidis as a very dominant species (n ϭ 66; 84% of the isolates) and with some less frequently encountered species, i.e., S. hominis (n ϭ 4), S. capitis (n ϭ 3), S. lugdunensis

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“…The type strain of this species is strain MB4 (= CCM 4175). (5,11,21,26). Most of the new species were isolated from animals; the exceptions were S .…”

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confidence: 99%

“…A total of 28 species are currently recognized in the genus Staphylococcus; 19 species are listed in Bergey's Manual of Systematic Bacteriology (14), two species (Staphylococcus chromogenes and Staphylococcus lentus) have been elevated from the subspecies level (8, 19), and 7 species have been described since 1984 (Staphylococcus arlettae, Staphylococcus delphini, Staphylococcus equorum, Staphylococcus felis, Staphylococcus kloosii, Staphylococcus lugdunensis, and Staphylococcus schleiferi) (5,11,21,26). Most of the new species were isolated from animals; the exceptions were S .…”

mentioning

confidence: 99%

Staphylococcus muscae, a New Species Isolated from Flies

Hájek

Ludwig

Schleifer

et al. 1992

International Journal of Systematic Bacteriology

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A new coagulase-negative species of the genus Staphylococcus, Staphylococcus muscae, is described on the basis of the results of a study of four strains that were isolated from flies. 16s rRNA sequences of the type strains of S . muscae, Staphylococcus schleiferi, and staphylococcus sciuri were determined and used, together with the corresponding sequences of Staphylococcus aureus and Staphylococcus epidermidis, for a comparative analysis. The new species is characterized taxonomically; this species is differentiated from the other novobiocin-susceptible staphylococci by its physiological and biochemical activities, cell wall composition, and levels of genetic relatedness. The type strain of this species is strain MB4 (= CCM 4175). (5,11,21,26). Most of the new species were isolated from animals; the exceptions were S . lugdunensis and S. schleiferi, which were isolated from humans. The descriptions of all 28 Staphylococcus species were based on studies of strains that were isolated from mammals, birds, and foodstuffs of animal origin.In this paper, we describe a new coagulase-negative species of staphylococci that was obtained from the body surfaces of flies. These organisms were found in about 7% of the flies caught in certain cow sheds but not on flies caught in human dwellings, stables, or piggeries (7). For this reason, this bacterium should be regarded as a transient rather than a resident on flies.In this report we present phenetic and molecular taxonomic data for a new species, Staphylococcus muscae. MATERIALS AND METHODSBacterial strains. Four strains were isolated from flies trapped in cow sheds; strains MB4T, (T = type strain), MB21, and MB50 were isolated from Stomoxys calcitrans, and strain MB30 was isolated from Musca domestica (7). The medium used for isolation was blood agar base no. 2 (Oxoid) supplemented with 5% defibrinated ovine blood. Nutrient agar (Oxoid) containing glucose (2%, wt/vol) and P agar (15) were used to propagate all of the isolates. Inocula for tests were prepared from 1-day cultures that were incubated at 37°C. All strains were stored as frozen suspensions in glycerol broth at -25°C (10) and also in a freezedried state.Methods. The procedures used for determining morphological, physiological, and biochemical characteristics have been described elsewhere (6). The type strains of the 14 novobiocin-susceptible Staphylococcus species (see Table 2) * Corresponding author. 97were obtained from the Deutsche Sammlung von Mikroorganismen (DSM), Braunschweig, Germany, the American Type Culture Collection (ATCC), Rockville, Md., and the Czechoslovak Collection of Microorganisms (CCM), Brno, Czechoslovakia.Phage typing was carried out by using the method of Blair and Williams (1). We used 24 phages (25) for human Staphylococcus aureus strains, 12 phages (24) for bovine S. aureus strains, 4 experimental phages (2) for Staphylococcus intermedius strains, and 4 phages (9) for Staphylococcus hyicus strains.We used the methods described by Schleifer and Kandler (20) and Schleifer (18) for cel...

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Staphylococcus lugdunensis sp. nov. and Staphylococcus schleiferi sp. nov., Two Species from Human Clinical Specimens

Cited by 360 publications

References 28 publications

Evaluation of species-specific score cut-off values for various Staphylococcus species using a MALDI Biotyper-based identification

Evaluation of species-specific score cut-off values for various Staphylococcus species using a MALDI Biotyper-based identification

Tolerance to the Glycopeptides Vancomycin and Teicoplanin in Coagulase-Negative Staphylococci

Staphylococcus muscae, a New Species Isolated from Flies

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