Staphylococcus epidermidis Contributes to Healthy Maturation of the Nasal Microbiome by Stimulating Antimicrobial Peptide Production

Liu, Qian; Liu, Qingyun; Meng, Hongping; Lv, Huiying; Liu, Yao; Liu, Junlan; Wang, Hua; He, Lei; Qin, Juanxiu; Wang, Yanan; Dai, Yingxin; Otto, Michael; Li, Min

doi:10.1016/j.chom.2019.11.003

Cited by 110 publications

(95 citation statements)

References 64 publications

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“…It has been shown that S. epidermidis colonization of skin induces AMP production by keratinocytes (5,7,8,65). Our findings regarding induction of LL37 and Defβ4 during early phases of human skin colonization with S. epidermidis are in line with these results.…”

Section: Discussionsupporting

confidence: 91%

“…One of the most common members of the healthy cutaneous microbiome is Staphylococcus epidermidis. S. epidermidis stimulates antimicrobial peptide production by skin cells (4)(5)(6)(7)(8)(9)(10)(11), which may provide protection against pathogenic bacteria (4,5,(10)(11)(12). Recent studies reported that colonization of mouse skin with S. epidermidis induced commensal-specific tissue (skin)-resident memory T cells that demonstrated immunoregulatory and tissue repair properties.…”

Section: Introductionmentioning

confidence: 99%

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Staphylococcus epidermidis Boosts Innate Immune Response by Activation of Gamma Delta T Cells and Induction of Perforin-2 in Human Skin

et al. 2020

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Perforin-2 (P-2) is an antimicrobial protein with unique properties to kill intracellular bacteria. Gamma delta (GD) T cells, as the major T cell population in epithelial tissues, play a central role in protective and pathogenic immune responses in the skin. However, the tissue-specific mechanisms that control the innate immune response and the effector functions of GD T cells, especially the cross-talk with commensal organisms, are not very well understood. We hypothesized that the most prevalent skin commensal microorganism, Staphylococcus epidermidis, may play a role in regulating GD T cellmediated cutaneous responses. We analyzed antimicrobial protein P-2 expression in human skin at a single cell resolution using an amplified fluorescence in situ hybridization approach to detect P-2 mRNA in combination with immunophenotyping. We show that S. epidermidis activates GD T cells and upregulates P-2 in human skin ex vivo in a cell-specific manner. Furthermore, P-2 upregulation following S. epidermidis stimulation correlates with increased ability of skin cells to kill intracellular Staphylococcus aureus. Our findings are the first to reveal that skin commensal bacteria induce P-2 expression, which may be utilized beneficially to modulate host innate immune responses and protect from skin infections.

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Staphylococcus epidermidis Boosts Innate Immune Response by Activation of Gamma Delta T Cells and Induction of Perforin-2 in Human Skin

et al. 2020

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“…DNA was extracted from microbial communities as described in Vilchez-Vargas et al 91 . Sequencing of the V3-V4 region of the 16S rRNA gene with forward primer (341F) and reverse primer (785R) was done on an Illumina MiSeq in PE 2X300 bp using V3 chemistry (Illumina) by Biofidal (Vaulx-en-Velin, France-https ://www.biofi dal-lab.com), as described in Supplementary Method (6).…”

Section: Flow Cytometric Fingerprinting and Classification Of Populatmentioning

confidence: 99%

“…The amplicon data were processed as described in 92 using mothur (v.1.39.5), largely based on the protocol by the Schloss lab 93,94 and described in more detail in Supplementary Method (6). Richness was estimated using the Chao1 index 95 and diversity was estimated the Inverse Simpson index.…”

Section: Flow Cytometric Fingerprinting and Classification Of Populatmentioning

confidence: 99%

Lacticaseibacillus casei AMBR2 modulates the epithelial barrier function and immune response in a donor-derived nasal microbiota manner

Rudder

García‐Timermans

Boeck

et al. 2020

Sci Rep

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Live biotherapeutic products (LBP) are emerging as alternative treatment strategies for chronic rhinosinusitis. The selection of interesting candidate LBPs often involves model systems that do not include the polymicrobial background (i.e. the host microbiota) in which they will be introduced. Here, we performed a screening in a simplified model system of upper respiratory epithelium to assess the effect of nasal microbiota composition on the ability to attach and grow of a potential LBP, Lacticaseibacillus casei AMBR2, in this polymicrobial background. After selecting the most permissive and least permissive donor, L. casei AMBR2 colonisation in their respective polymicrobial backgrounds was assessed in more physiologically relevant model systems. We examined cytotoxicity, epithelial barrier function, and cytokine secretion, as well as bacterial cell density and phenotypic diversity in differentiated airway epithelium based models, with or without macrophage-like cells. L. casei AMBR2 could colonize in the presence of both selected donor microbiota and increased epithelial barrier resistance in presence of donor-derived nasal bacteria, as well as anti-inflammatory cytokine secretion in the presence of macrophage-like cells. This study highlights the potential of L. casei AMBR2 as LBP and the necessity to employ physiologically relevant model systems to investigate host–microbe interaction in LBP research.

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“…Studies in men suggest that the NM is a key factor in maintaining respiratory health by affecting both resistance to pathogens and immunological responses [19][20][21] . While a greater mucosal diversity may play a role in limiting in ammation and protecting against infections [22][23][24] , potential microbial species known as "keystone species" may also have a bene cial effect on the ecosystem's balance, function and health 21,25,26 .…”

Section: Introductionmentioning

confidence: 99%

Variations in Facial Conformation Are Associated With Differences in Nasal Microbiota in Healthy Dogs

Vangrinsven

Fastrès

Taminiau

et al. 2020

Preprint

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Background – Extrinsic and intrinsic factors have been shown to influence nasal microbiota (NM) in humans. Very few studies investigated the association between nasal microbiota and factors like facial/body conformation, age, and environment in dogs. Objectives are to investigate variations in NM in healthy dogs with different facial and body conformations and to assess the influence of age and living environment. A total of 46 dogs of different age, living environment and from 3 different breed groups were recruited: 22 meso-/dolichocephalic medium to large breed dogs, 12 brachycephalic dogs and 12 terrier breeds. The nasal bacterial microbiota was assessed through sequencing of 16S rRNA gene (V1-V3 regions) amplicons.Results – We showed major differences in the NM composition together with increased richness and α-diversity in brachycephalic dogs, compared to meso-/dolichocephalic dogs and dogs from terrier breeds. We failed to detect any effect of age or environment.Conclusion – Healthy brachycephalic breeds and their unique facial conformation is associated with a distinct NM profile. Description of the NM in healthy dogs serves as a foundation for future researches assessing the changes associated with disease and the modulation of NM communities as a potential treatment.

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Staphylococcus epidermidis Contributes to Healthy Maturation of the Nasal Microbiome by Stimulating Antimicrobial Peptide Production

Cited by 110 publications

References 64 publications

Staphylococcus epidermidis Boosts Innate Immune Response by Activation of Gamma Delta T Cells and Induction of Perforin-2 in Human Skin

Staphylococcus epidermidis Boosts Innate Immune Response by Activation of Gamma Delta T Cells and Induction of Perforin-2 in Human Skin

Lacticaseibacillus casei AMBR2 modulates the epithelial barrier function and immune response in a donor-derived nasal microbiota manner

Variations in Facial Conformation Are Associated With Differences in Nasal Microbiota in Healthy Dogs

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