Staphylococcus coagulasa-negativa clínicamente significativos: Especies más frecuentes y factores de virulencia

Fariña, Norma; Carpinelli, Letizia; Samudio, Margarita; Guillén, Rosa; Laspina, Florentina; Sanabria, R; Abente, Sonia; Rodas, Ladis; González, Pilar; Kaspar, Herminia Miño de

doi:10.4067/s0716-10182013000500003

Cited by 24 publications

(15 citation statements)

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“…Of all known pathogenic Vibrio of major medical interest listed by Daniels and Shafaie (2000), only V. parahaemolyticus was present in our oyster samples. Among those isolated from surface waters, the coagulase-negative Staphylococcus warneri and S. cohnii are of mild clinical interest as possible sources of nosocomial infections (Martínez and Máttar 2006, Fariña et al 2013, Soldera et al 2013, while Lysobacter spongiicola pertains to a genus of potential importance for its production of several extracellular enzymes (de Bruijn et al 2015), and because several Lysobacter species have been suggested for biological plant disease control (Hashizume et al 2004, Li et al 2008.…”

Section: Discussionmentioning

confidence: 99%

BACTERIAL COMMUNITIES OF THE OYSTERS Crassostrea corteziensis AND C. sikamea OF COSPITA BAY, SINALOA, MEXICO

Luis-Villaseñor

Zamudio-Armenta

Voltolina

et al. 2018

Rev. Int. Contam. Ambie.

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This work aimed to quantify the bacterial loads and determine the taxonomic composition of the microbial communities of oysters Crassostrea corteziensis and C. sikamea, as well as the surrounding waters of Cospita Bay, using two sets of samples (M1 and M4) obtained in the dry season and two (M2 and M3) in the rainy season. Bacterial loads were quantified with the viable count technique and out of the 320 strains isolated for their different morphologies, 84 were identified with molecular techniques to the species level. These species pertained to the families Vibrionaceae, Bacillaceae, Brucellaceae, Micrococcaceae, Pseudoalteromonaceae, Rhodobactereceae, Shewanellaceae and Staphylococcaceae. The higher concentrations of total bacteria were in the rainy season (samples M2 and M3), when families Vibrionaceae and Brucellaceae dominated. The highest Vibrio spp. and Pseudomonas spp. concentrations were in winter (samples M4). Probably because of its poor health conditions, the Kumamoto oyster C. sikamea had the highest diversity of bacterial species and the highest number of known pathogens for aquatic organisms. Palabras clave: bacterias, ostiones cultivados, ostiones silvestres RESUMEN En la presente investigación se cuantificó la carga bacteriana y se determinó la composición de la microbiota de los ostiones Crassostrea corteziensis y C. sikamea, así como del agua circundante de Bahía Cospita, usando dos muestras obtenidas durante el periodo de estiaje (muestreos M1 y M4) y dos obtenidas en el periodo de lluvias (muestreos M2 y M3). La carga bacteriana se cuantificó con la técnica de cuenta viable y se aislaron con criterios morfológicos 320 cepas, de las cuales de 84 se identificaron con técnicas moleculares hasta el nivel de especie y fueron agrupadas en las familias Vibrionaceae, Bacillaceae, Brucellaceae, Micrococcaceae,

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Section: Discussionmentioning

confidence: 99%

BACTERIAL COMMUNITIES OF THE OYSTERS Crassostrea corteziensis AND C. sikamea OF COSPITA BAY, SINALOA, MEXICO

Luis-Villaseñor

Zamudio-Armenta

Voltolina

et al. 2018

Rev. Int. Contam. Ambie.

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“…Antibiotic resistance remains a major threat to public health worldwide (10). Although other studies showed that the most prevalent spices was S. epidermidis (11,12), the results of the present study showed that S. hominis and S. haemolyticus, as the most important causes of nosocomial infections, had the highest prevalence (10). Insufficient hand hygiene and inadequate disinfection and/or sterilization of medical instruments and surfaces may also be assumed as causes of the distribution of coagulase-negative Staphylococci (CoNS) such as S. hominis and S. haemolyticus in the hospital settings (13).…”

Section: Discussionmentioning

confidence: 99%

Distribution of Aminoglycoside Resistance Genes in Coagulase-Negative Staphylococci Isolated From Hospitalized Patients

Sabzehali

Goudarzi

et al. 2017

Arch Pediatr Infect Dis

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Background: The incidence of coagulase-negative staphylococci (CNS) isolates, as causes of hospital-acquired infections, is increasing annually and it is a truly global challenge. Objectives: The current study aimed at assessing aminoglycoside resistance genes among CNS isolated from hospitalized patients and investigating the prevalence of methicillin and aminoglycoside resistance genes using molecular methods. Methods: A total of 103 species of coagulase negative staphylococci were isolated from clinical specimens from August 2013 to November 2014. All the specimens were identified using conventional microbiological methods including colony morphology, Gram staining, catalase, and slide and tube coagulase. The isolates were subjected to the API-20 Staph identification kit. Antibiotic susceptibility tests were performed using Kirby-Bauer disc diffusion method. The methicillin and aminoglycoside resistant genes among coagulase negative staphylococci were detected by polymerase chain reaction (PCR) and sequencing methods. Results: The most frequent clinically isolated coagulase negative staphylococci species were Staphylococcus hominis and S. haemolyticus (51.9%), S. epidermidis (21.7%), S. lugdunensis (9.8%), S. intermedius (4.9%), S. saprophyticus and S. simulans (3.9%), S. warneri (2.9%), as well as S. chromogenes, S. sciuri, S. caprae, S. schleiferi, and S. xylosus (0.98%). The overall rate of methicillin resistance among CNS species in the present study was 89 (86.4%). The resistance of CNS isolates against tested antibiotics was as follows: 74 (71.8%) to cefoxitin, 54 (52.4%) to kanamycin, 51 (49.5%) to gentamicin, 45 (43.7%) to tobramycin, and 16 (15.5%) to amikacin. The prevalence of aminoglycoside resistance genes such as ant (4')-Ia, aac (6')/aph (2") and aph (3')-IIIa were 89 (86.4%), 87(84.5%), and 68(66%), respectively. Conclusions: The presence of antibiotic resistance among coagulase negative Staphylococcus species, which cause nosocomial infections, has increased. Therefore, identification of coagulase negative staphylococci within 24 hours after development of enzymatic tests would be very useful in any clinical microbiology laboratory for effective treatment. Resistance to antibiotics, including aminoglycosides, develops quickly in CNS species where these antimicrobial agents are widely used. Thus, a better understanding of antibiotic resistance of CNS species is essential to eliminate antibiotic resistance in hospitals and society.

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“…La prueba de sensibilidad antimicrobiana se hizo siguiendo el método de difusión en disco Kirby-Bauer. Se hizo una dilución bacteriana semejante a la escala de turbidez de 0.5 de McFarland y la muestra fue sembrada en el agar Müller-Hinton con la ayuda de un hisopo estéril (Fariña et al, 2013). Se seleccionaron los antibióticos más usados en la clínica de animales de compañía para el tratamiento de otitis externa.…”

Section: Susceptibilidad Antimicrobianaunclassified

Resistencia antimicrobiana en Staphylococcus coagulasa positiva (CoPS) aislados de perros con otitis externa

Palomino-Farfán

Alvarez

Siuce

et al. 2020

Rev. investig. vet. Perú

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El grupo de Staphylococcus coagulasa positiva (CoPS) incluye a las especies S. aureus, S. pseudintermedius, S. intermedius y a S. schleiferi subsp. coagulans. El propósito de este estudio fue evaluar la resistencia antimicrobiana de CoPS aislados de perros con otitis externa, utilizando los antibióticos más usados en la clínica diaria para tratar esta enfermedad. Se obtuvieron 148 muestras de secreciones óticas provenientes de 104 perros con otitis externas que fueron atendidos en una clínica veterinaria de lima, Perú, en un periodo de seis meses. Se identificaron 113 aislados como Staphylococcus sp, de las cuales, 105 fueron CoPS. Las cepas presentaron la mayor resistencia a la ciprofloxacina (27.4%) y la mayor sensibilidad a la nitrofurantoína (82.1%).

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Staphylococcus coagulasa-negativa clínicamente significativos: Especies más frecuentes y factores de virulencia

Abstract: The frequency of S. lugdunensis, the most virulent coagulase-negative staphylococci species, was relatively high. The main virulence factor in S. epidermidis was biofilm production, being higher in those resistant to methicillin.

Cited by 24 publications

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BACTERIAL COMMUNITIES OF THE OYSTERS Crassostrea corteziensis AND C. sikamea OF COSPITA BAY, SINALOA, MEXICO

BACTERIAL COMMUNITIES OF THE OYSTERS Crassostrea corteziensis AND C. sikamea OF COSPITA BAY, SINALOA, MEXICO

Distribution of Aminoglycoside Resistance Genes in Coagulase-Negative Staphylococci Isolated From Hospitalized Patients

Resistencia antimicrobiana en Staphylococcus coagulasa positiva (CoPS) aislados de perros con otitis externa

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