Staphylococcus aureus phagocytosis

Cantinieaux, Brigitte; Hariga, C.; Courtoy, Pierre J.; J, Hupin; Fondu, Pierre

doi:10.1016/0022-1759(89)90161-0

Cited by 55 publications

(4 citation statements)

References 15 publications

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“…For the staining of living bacteria and yeasts we used calcein‐AM, which appeared to be most suitable after comparison of different dyes for microorganisms. Previously used fluorescence stains such as FITC [1–4,6,20] and BCECF‐AM [5–11] showed a high sensitivity for pH changes, which occur in the phagolysosomes and cause a change in the emitted wavelength of light. Others, such as Texas Red [21], showed toxic effects on the microorganisms (data not shown).…”

Section: Discussionmentioning

confidence: 99%

Simultaneous cytofluorometric measurement of phagocytosis, burst production and killing of human phagocytes using Candida albicans and Staphylococcus aureus as target organisms

Husfeld

Adam

2000

Clinical Microbiology and Infection

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Section: Discussionmentioning

confidence: 99%

Simultaneous cytofluorometric measurement of phagocytosis, burst production and killing of human phagocytes using Candida albicans and Staphylococcus aureus as target organisms

Husfeld

Adam

2000

Clinical Microbiology and Infection

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“…FITC‐labeled MRSA were prepared by a previous method. [ 28 ] Blank liposome‐opsonized MRSA were obtained by incubating MRSA with functional blank liposomes (PEGLs, TatLs, WGALs, or CMLs) in DMEM containing 10% FBS for 15 min. MRSA treated by DMEM containing 10% FBS were selected as a control.…”

Section: Methodsmentioning

confidence: 99%

“…RAW264.7 cells (liposome‐treated or untreated) were cultured with MRSA (liposome‐opsonized or nonopsonized) at MOI of 20 for 1.5 h, and then the phagocytic efficiency expressed by the fluorescence intensity of FITC was detected by flow cytometry. [ 28 ] In SIM imaging study, MRSA were stained by Hoechst 33258. RAW264.7 cells and liposomal C6‐treated MRSA that was acquired by the same method as the blank liposomes‐opsonized MRSA were cocultivated in a 12 mm imaging dish for 1.5 h. After RAW264.7 cells were labeled by ER‐Tracker Red dye, fluorescent images were visualized by an N‐SIM S super resolution microscope (Nikon, Japan).…”

Section: Methodsmentioning

confidence: 99%

A New Anti‐Immune Evasion Strategy against Methicillin‐Resistant Staphylococcus Aureus (MRSA) Infections: Simulating Complement Immunotherapy Based on Complement‐Mimic Antibiotic Delivery System

Hou

Zhang

Meng

et al. 2020

Advanced Therapeutics

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Methicillin-resistant Staphylococcus aureus (MRSA) can interfere with synergistic host defenses, thus escaping from immune surveillance. Herein, a new immunotherapy strategy-simulating complement therapy is proposed. Two artificial effectors, Tat cell penetrating peptide and wheat germ agglutinin, are utilized to develop a multifunctional complement-mimic liposome (CML) simultaneously delivering antibiotics and orchestrating the complement system with macrophages. The CML successfully simulates the functions of the complement system, including formation of a membrane attack complex, mediation of opsonophagocytosis, and activation of phagocytes. At infection sites, CMLs are able to recognize MRSA and disrupt bacterial permeation barriers (cell walls and cell membranes). Meanwhile, CMLs attached to the surface of MRSA are able to activate the phagocytosis and immune responses of macrophages. As a result, CMLs significantly increased the bacterial activity of clarithromycin both in vitro and in macrophages. Moreover, CMLs effectively reduced the MRSA burden in three infection models, including skin abscesses, pneumonia, and bacteremia mouse models. Therefore, the CML provides a novel strategy for overcoming bacterial immune evasion and sheds light on the development of immunotherapies for infectious diseases.

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“…Using Robinson et al's work [ 11 ] as a guide, fluorescein isothiocyanate (FITC) labeling of yeast cells was carried out according to the protocol of Cantinieaux et al [ 12 ] with modifications. Briefly, yeast were harvested from growth media and suspended in carbonate buffer (0.5 M sodium carbonate/sodium bicarbonate, pH 9.5) and cell concentration adjusted to an optical density of 0.74 AU at 620 nm.…”

Section: Methodsmentioning

confidence: 99%

Adherence and Blocking of Candida Albicans to Cultured Vaginal Epithelial Cells: Treatments to Decrease Adherence

Hollmer

Essmann

Ault

et al. 2006

Infectious Diseases in Obstetrics and Gynecology

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Background. Pathogenesis of mucosal microorganisms depends on adherence to the tissues they colonize and infect. For Candida albicans, cell surface hydrophobicity may play a significant role in tissue binding ability. Methods. A continuous cell line of vaginal epithelial cells (VEC) was grown in keratinocyte serum-free medium (KSFM) with supplements and harvested by trypsinization. VEC were combined with yeast cells to evaluate adherence and inhibition of adherence. In this experimental setup, yeast stained with fluorescein isothiocyanate were allowed to attach to VEC and the resulting fluorescent VEC were detected by flow cytometry. Results. VEC were cultured and examined daily after plating and showed morphology similar to basal epithelial cells. Culture media supplemented with estradiol showed increased VEC proliferation initially (first 24 h) but cell morphology was not altered. Fluorescinated Candida cells bound effectively to the cultured VEC. Using fresh cells exposed to various preparations of K-Y, we showed that all formulations of the product reduced Candida binding to VEC by 25% to 50%. While VEC were generally harvested for use in experiments when they were near confluent growth, we allowed some cultures to grow beyond that point and discovered that cells allowed to become overgrown or stressed appeared to bind yeast cells more effectively. Conclusion. Flow cytometry is a useful method for evaluating binding of stained yeast cells to cultured VEC and has demonstrated that commercially available products have the ability to interfere with the process of yeast adherence to epithelial cells.

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Staphylococcus aureus phagocytosis

Cited by 55 publications

References 15 publications

Simultaneous cytofluorometric measurement of phagocytosis, burst production and killing of human phagocytes using Candida albicans and Staphylococcus aureus as target organisms

Simultaneous cytofluorometric measurement of phagocytosis, burst production and killing of human phagocytes using Candida albicans and Staphylococcus aureus as target organisms

A New Anti‐Immune Evasion Strategy against Methicillin‐Resistant Staphylococcus Aureus (MRSA) Infections: Simulating Complement Immunotherapy Based on Complement‐Mimic Antibiotic Delivery System

Adherence and Blocking of Candida Albicans to Cultured Vaginal Epithelial Cells: Treatments to Decrease Adherence

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