Staphylococcus aureus of phage type 187 isolated from people occurred to be a genes carrier of eneterotoxin C and toxic shock syndrome toxin-1 (TSST-1)

Piechowicz, Lidia; Garbacz, Katarzyna; Galiński, J

doi:10.1016/j.ijheh.2007.06.010

Cited by 7 publications

(3 citation statements)

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“…The homology and the similar gene organization of tagV and tagN in S. pseudintermedius , which colonizes in particular dogs (41), further substantiates this hypothesis. Nevertheless, recent reports on ST395 from noses or infections of hospital patients in northern Germany and Poland (42, 43) indicate that ST395 clones are at least partly adapted to the human host. Since WTA has been found to be crucial for host-pathogen interaction (3), the discovery of tagN can help to unravel the role of WTA glycosyl residues in host-pathogen interaction and host specificity in the future.…”

Section: Discussionmentioning

confidence: 99%

Biosynthesis of the Unique Wall Teichoic Acid of Staphylococcus aureus Lineage ST395

Winstel

Sanchéz-Carballo

Holst

et al. 2014

mBio

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The major clonal lineages of the human pathogen Staphylococcus aureus produce cell wall-anchored anionic poly-ribitol-phosphate (RboP) wall teichoic acids (WTA) substituted with d-Alanine and N-acetyl-d-glucosamine. The phylogenetically isolated S. aureus ST395 lineage has recently been found to produce a unique poly-glycerol-phosphate (GroP) WTA glycosylated with N-acetyl-d-galactosamine (GalNAc). ST395 clones bear putative WTA biosynthesis genes on a novel genetic element probably acquired from coagulase-negative staphylococci (CoNS). We elucidated the ST395 WTA biosynthesis pathway and identified three novel WTA biosynthetic genes, including those encoding an α-O-GalNAc transferase TagN, a nucleotide sugar epimerase TagV probably required for generation of the activated sugar donor substrate for TagN, and an unusually short GroP WTA polymerase TagF. By using a panel of mutants derived from ST395, the GalNAc residues carried by GroP WTA were found to be required for infection by the ST395-specific bacteriophage Φ187 and to play a crucial role in horizontal gene transfer of S. aureus pathogenicity islands (SaPIs). Notably, ectopic expression of ST395 WTA biosynthesis genes rendered normal S. aureus susceptible to Φ187 and enabled Φ187-mediated SaPI transfer from ST395 to regular S. aureus. We provide evidence that exchange of WTA genes and their combination in variable, mosaic-like gene clusters have shaped the evolution of staphylococci and their capacities to undergo horizontal gene transfer events.

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Section: Discussionmentioning

confidence: 99%

Biosynthesis of the Unique Wall Teichoic Acid of Staphylococcus aureus Lineage ST395

Winstel

Sanchéz-Carballo

Holst

et al. 2014

mBio

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“…In contrast to the vast majority of S. aureus clonal lineages possessing polyribitol phosphate wall teichoic acid (WTA) [71], the unusual polyglycerol phosphate WTA-producing S. aureus PS 187, which belongs to a distant S. aureus lineage (phage type 187, biotype E) often isolated from dogs [72], was less susceptible to PRF-119. Thus, changes of the cell wall composition or structure may influence the endolysin activity [59].…”

Section: Discussionmentioning

confidence: 99%

In Vitro Activity of the Bacteriophage Endolysin HY-133 against Staphylococcus aureus Small-Colony Variants and Their Corresponding Wild Types

Schleimer

Kaspar

Knaack

et al. 2019

IJMS

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Nasal carriage of methicillin-susceptible (MSSA) and methicillin-resistant Staphylococcus aureus (MRSA) represents both a source and a risk factor for subsequent infections. However, existing MRSA decolonization strategies and antibiotic treatment options are hampered by the duration of administration and particularly by the emergence of resistance. Moreover, beyond classical resistance mechanisms, functional resistance as the formation of the small-colony variant (SCV) phenotype may also impair the course and treatment of S. aureus infections. For the recombinant bacteriophage endolysin HY-133, rapid bactericidal and highly selective in vitro activities against MSSA and MRSA has been shown. In order to assess the in vitro efficacy of HY-133 against the SCV phenotype, minimal inhibitory (MIC) and minimal bactericidal concentrations (MBC) were evaluated on clinical SCVs, their isogenic wild types, as well as on genetically derived and gentamicin-selected SCVs. For all strains and growth phases, HY-133 MIC and MBC ranged between 0.12 and 1 mg/L. Time-kill studies revealed a fast-acting bactericidal activity of HY-133 resulting in a ≥3 − log10 decrease in CFU/mL within 1 h compared to oxacillin, which required 4–24 h. Since the mode of action of HY-133 was independent of growth phase, resistance pattern, and phenotype, it is a promising candidate for future S. aureus decolonization strategies comprising rapid activity against phenotypic variants exhibiting functional resistance.

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“…Estas enterotoxinas são antígenos poderosos que estimulam a proliferação de células T não específicas. Também são resistentes à enzimas proteolíticas e estáveis ao calor, apresentando peso molecular que varia entre 27 e 31 kDa (MEHROTRA et al, 2000;KAMBOJ et al, 2006;NEMA et al, 2007;PIECHOWICZ et al, 2008) e são secretadas, principalmente, durante a fase de crescimento pós-exponencial (QIU et al, 2010).…”

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Ação do ácido indol-3-acético sobre Staphylococcus aureus

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Aç çã ão o d do o á ác ci id do o i in nd do ol l--3 3--a ac cé ét ti ic co o s so ob br re e S St ta ap ph hy yl lo oc co oc cc cu us s a au ur re eu us s Pirassununga 2012 Andréia Cristina Nakashima Vaz A Aç çã ão o d do o á ác ci id do o i in nd do ol l--3 3--a ac cé ét ti ic co o s so ob br re e S St ta ap ph hy yl lo oc co oc cc cu us s a au ur re eu us s vi AGRADECIMENTOS À Deus, por ter me dado a graça de viver... por ter me dado a força de lutar pelos meus desejos... por ter colocado pessoas tão especiais em minha vida ... por ter me dado oportunidades de viver e crescer! À Fundação de Amparo à Pesquisa do Estado de São Paulo -FAPESP, pelo financiamento do projeto de pesquisa e incentivo à experimentação acadêmica.À CAPES, pela bolsa de estudos.À Profa. Dra. Mariza Pires de Melo, pela oportunidade de trabalhar com pessoas tão queridas.Aos professores da Faculdade de Zootecnia e Engenharia de Alimentos, pelos ensinamentos.Ao Prof. Dr. Heidge Fukumasu, pelos ensinamentos, apoio e, claro, pela paciência de ter que me aturar batendo repetidas vezes na porta de sua sala.

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Staphylococcus aureus of phage type 187 isolated from people occurred to be a genes carrier of eneterotoxin C and toxic shock syndrome toxin-1 (TSST-1)

Cited by 7 publications

References 20 publications

Biosynthesis of the Unique Wall Teichoic Acid of Staphylococcus aureus Lineage ST395

Biosynthesis of the Unique Wall Teichoic Acid of Staphylococcus aureus Lineage ST395

In Vitro Activity of the Bacteriophage Endolysin HY-133 against Staphylococcus aureus Small-Colony Variants and Their Corresponding Wild Types

Ação do ácido indol-3-acético sobre Staphylococcus aureus

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