Staphylococcus aureus isolated from selected dairies of Algeria: Prevalence and susceptibility to antibiotics

Abstract

Aim: The objectives of this study were to assess the prevalence of Staphylococcus aureus in raw milk in Algerian dairies, to study the effect of seasons on the contamination of milk and the susceptibility of isolated strains to antibiotics, and to estimate the risk on the health consumer. Materials and Methods: The ISO method 6888-1 (1) was used for Staphylococcus screening. Antimicrobial susceptibility to the 11most used antibiotics in veterinary medicine was assessed using the disk diffusion assay. Results: The overall prevalence was 31.56% (95/301); 34.84% (85/244) from raw milk collectors cisterns (MCC), 22.73% (5/22) from mixing tank milk before pasteurization, and 14.29% (5/35) from pasteurized tank milk (p<0.05). A significant difference (p<0.001) of contamination on MCC was observed between dairies without season influence (p≥0.05). It was observed that 49.47% of S. aureus isolates were resistant to penicillin, 5.26% to tetracycline, 4.21% to erythromycin, 3.15% to neomycin, 2.10% to cefoxitin, 2.10% to clindamycin, and 1.05% to ofloxacin. No resistance was observed for vancomycin, gentamicin, chloramphenicol, and trimethoprim-sulfamethoxazole. Conclusion: A high prevalence of S. aureus from MCC was observed without significant effect of season. The pasteurization does not ensure the elimination of bacteria in all samples. Half of the isolates were resistant to penicillin. These findings emphasize the importance of S. aureus control in Algerian milk industry at different levels to improve public health.

Comparison of Antibiotic Resistance Profile and Biofilm Production of Staphylococcus aureus Isolates Derived from Human Specimens and Animal-Derived Samples

“…With regards to animal-derived isolates, most strains showed resistance to penicillin (52%) and tetracycline (33%) in according with previous works [11,17,20,21,22,23]. By comparing our results with those obtained in a previous study [6] which reported the antimicrobial profile of 80 isolates collected between 1998–2014, we found an increase of resistance to penicillin (52% vs. 35.7%), tetracycline (33% vs. 20%), ceftriaxone (14% vs. 3.7%), and gentamicin (14% vs. 4%), which suggested the spread of resistance.…”

Epidemiological study of sausage in Algeria: Prevalence, quality assessment, and antibiotic resistance of Staphylococcus aureus isolates and the risk factors associated with consumer habits affecting foodborne poisoning

“…For dairy product studies, in Algeria, similar results were reported, as described by [20,43,44]. In contrast, the prevalence obtained was higher than that found in Egypt (12%) [45], (16.60%) [23], Nigeria (9.15%) [46], China (1.8%) [22], Greece (18%) [27], Spain (7%) [26], and Italy (9.79%) [47] (11.77%) [29], with the highest prevalence registered in Georgia [12] and Turkey [48] with (63%) and (42.50%), respectively.…”

Antibiotic resistance of Staphylococcus aureus isolated from raw cow and goat milk produced in the Tiaret and Souk Ahras areas of Algeria

“…Chaalal et al [11] have reported a rate of 21.5% in various types of foods, including raw milk, meat, and pastries. Other studies conducted in Algeria have found a lower prevalence, with values of 4.1%−13.6% in raw milk and traditional dairy products [22][23][24], but, to the best of our knowledge, no study has examined goat milk.…”

“…Chaalal et al [11] have reported that 33.3% of the S. aureus strains mainly isolated from raw milk and raw meat were multi-drug resistant, with 45.7% resistant to one antibiotic and 20.0% sensitive to all the antibiotics tested [11]. Matallah et al [23] and Titouche et al [22] have reported that 3.15% and 23.18% of their strains were multi-resistant. Similar findings have been obtained in Italy [25] and Russia [35].…”