Staphylococcus aureus-human endothelial cell interactions

Fibronectin-binding protein acts as Staphylococcus aureus invasin via fibronectin bridging to integrin alpha5beta1

“…As seen for phagocytosis by professional phagocytes, microtubule inhibitors, even when given at high concentrations, only partially diminished invasion (5 mg ml À1 nocodazole and 5 mg ml À1 colchicine; data not shown). Transmission electron microscopy demonstrated the presence of S. aureus in phagosome-like intracellular structures (Hamill et al, 1986;Lowy et al, 1988;Fig. 1C and D) Taken together, these results con®rm that S. aureus invasion of 293 cells involves active cellular (but not bacterial) processes and bears some mechanistic resemblance to uptake by professional phagocytes.…”

A human endothelial cell membrane protein that binds Staphylococcus aureus in vitro.

“…A classic example ofthis process is acute infective endocarditis where pre- viously normal valves are colonized and subsequently destroyed (3,9). We, and others, have demonstrated that S. aureus adhere more avidly to human endothelial cells in tissue culture and to endovascular tissue than other bacterial species (10)(11)(12)(13)(14)(15). Investigators have speculated that this bacterial tropism may be the result of specific adhesin-receptor interactions (10).…”

“…However, in the absence ofantecedent injury, as is often the case in acute infective endocarditis or staphylococcal bacteremia, the initial adhesion interaction is with the endothelial cell surface (3,9). Staphylococci are capable of adhering to and penetrating endothelial cells in greater numbers than other bacterial species (10)(11)(12)(13)(14)(15). This affinity for endothelial cell tissue may be a consequence of adhesin-receptor interactions that occur between the bacteria and the endothelial cell surface (10,12,15).…”

Bacterial fibronectin-binding proteins and endothelial cell surface fibronectin mediate adherence of Staphylococcus aureus to resting human endothelial cells

“…S. aureus adheres to human endothelial cells in vitro (Vercellotti et al, 1984 ;Ogawa et al, 1985) but, surprisingly, both the endothelial surface receptor(s) and the bacterial-cell-wall-associated adhesin(s) responsible remain undefined. Once adhesion has occurred S. aureus cells undergo a process akin to phagocytosis (Lowy et al, 1988 ;Yao et al, 1995). This IP: 13.66.222.141…”