Staphylococcus aureus en Residentes de un Hogar de Ancianos de Cartagena

Bettin, Alfonso; Suárez, Paola; Bedoya, Andrés Felipe Roncancio; Reyes, Niradiz

doi:10.1590/s0124-00642008000400015

Cited by 8 publications

(6 citation statements)

References 21 publications

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“…Nasal samples were obtained with a sterile cotton swab and placed in modified Stuart transport medium (OXOID, England); they were then transported to our microbiology lab and processed within 4 hours. S. aureus isolates were identified by previously described methods (12). Antibiotic susceptibility was assessed by the disc diffusion method following Clinical Laboratory Standards Institute (CLSI) guidelines (13).…”

Section: Specimen Collection and Microbiological Methodsmentioning

confidence: 99%

Nasal carriage of Panton Valentine leukocidin-positive methicillin-resistant Staphylococcus aureus in healthy preschool children

Rebollo¹,

Ordoñez-Tapia²,

Herazo-Herazo³

et al. 2011

Rev. salud pública

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Objective Determining the prevalence of nasal carriage of S. aureus, both sensitive to methicillin and resistant to it, in preschool children and evaluating the presence of Panton-Valentine leukocidin genes in the isolates. Methods This was a cross-sectional study in which cultures from anterior nares were obtained from healthy preschool children. Isolates were identified as S. aureus based on morphological and biochemical tests. Antibiotic susceptibility profiles were determined by the disk diffusion method. All the isolates were further analyzed by multiplex PCR to determine the presence of mecA and PVL genes; methicillin-resistant isolates were also SCCmec typed by multiplex PCR. Results Overall S. aureus nasal colonization prevalence was 38.5 % and 4.8 % for methicillin-resistant strains. All the methicillin-resistant isolates carried the genes for PVL; two isolates possessed the SCCmec type IV, two were SCCmec type I and one was SCCmec type II. Conclusion This study revealed high PVL-positive, methicillin-resistant S. aureus colonization prevalence in healthy preschool children from Cartagena, which may play a key role in the epidemiology of community-associated infection by methicillin-resistant S. aureus in healthy children from this particular geographical area.

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Section: Specimen Collection and Microbiological Methodsmentioning

confidence: 99%

Nasal carriage of Panton Valentine leukocidin-positive methicillin-resistant Staphylococcus aureus in healthy preschool children

Rebollo¹,

Ordoñez-Tapia²,

Herazo-Herazo³

et al. 2011

Rev. salud pública

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“…Nasal and vaginal specimens were transported to the microbiology laboratory of the School of Medicine at University of Cartagena, and processed within 8 to 18 hours according to protocols described by Bettin et al 17 . Antibiotic susceptibility of isolates confirmed as SA was performed by the disc diffusion method following recommendations of the Clinical and Laboratory Standards Institute (CLSI).…”

Section: Laboratory Methodsmentioning

confidence: 99%

Nasal and vaginal colonization of methicillin-resistant Staphylococcus aureus in pregnant women in Cartagena, Colombia

Correa¹,

Delgado²,

Rangel³

et al. 2012

Colomb Med

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Introduction: The host niche for Staphylococcus aureus (SA) are the anterior nares; however, vaginal colonization rates between 14% and 17.1% in pregnant women have been recently reported, raising interest about the potential risk in postpartum women and in neonates from colonized mothers. Objectives: To determine the prevalence of nasal and vaginal colonization of SA and the antibiotic susceptibility of the isolates in pregnant women attending a maternity hospital in Cartagena, Colombia. Methods: Nasal and vaginal swabs were obtained from participants and subjected to microbiological and molecular assays. A post discharge follow-up was performed for up to four weeks. Results: From 100 pregnant women enrolled in the study, 34 were colonized with SA; 29 only in the nares, three only in the vagina, and two at both sites. Colonization of pregnant women with SA was more common in the nares than in the vagina or at both sites [29/34 (85.3%) vs 3/34 (8.8%) and 2/34 (5.9%); p<0.05]. We obtained 36 SA isolates, nine (25%) of which were methicillin-resistant Staphylococcus aureus (MRSA), one was from the vagina; thus, the overall MRSA colonization rate among pregnant women was 9%. Molecular analysis showed that Panton-Valentine leukocidin (PVL) genes were carried by the vaginal MRSA, seven of the nasal MRSA, and two of the Methicillinsensitive Staphylococcus aureus (MSSA) isolates. Two MRSA isolates carried SCCmec type I and seven carried SCCmec type IV. Conclusions: Nasal colonization rate for SA in the study population was similar to previous reports. However, the frequency of nasal colonization of MRSA was higher while vaginal colonization of SA was lower than previously reported in other studies for similar populations. The MRSA isolates obtained showed a community profile.

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“…Diversos autores han reportado frecuencias de portación similares [2,4,14,17,20,21]. En contraste, Castro y cols., encontraron en una población escolar, una frecuencia de portación mayor al comparar estos resultados con los encontrados en el año 2010 y 2012 en los estudiantes de Bacteriología [18].…”

Section: Tabla 2 Factores De Riesgo En Portadores Deunclassified

“…La hospitalización es un factor de riesgo que permite la adquisición de cepas nosocomiales que posteriormente pueden ser transmitidas a las comunidades. Además el tratamiento con antibióticos, puede permitir la colonización de SARM, debido a que elimina las cepas SASM [18,20]. El compromiso respiratorio en los tres meses previos a la toma de la muestra; se considera una condición de riesgo debido a que es ampliamente conocido que los principales agentes productores de infecciones respiratorias son los virus; estos pueden dañar las células epiteliales ciliadas, lo cual puede favorecer la sobreinfección bacteriana y, por consiguiente, la colonización de S. aureus, por lo tanto, la presencia de estas infecciones favorece el estado de portador de esta bacteria [30,31].…”

Section: Tabla 2 Factores De Riesgo En Portadores Deunclassified

“…Existen diversas poblaciones con riesgo de presentar infección por esta bacteria tales como: escolares, presos, personas con cáncer, personal del área de la salud, estudiantes de medicina, entre otros [13,14,15,16,17,18,19,20]. En el año 2005, Castro y cols realizaron una investigación en estudiantes de quinto semestre de Bacteriología de la Corporación Universitaria Rafael Núñez, encontrando una frecuencia de portación de S. aureus de 29,8% [21]; en 2010 se evaluó nuevamente la portación en estudiantes de Bacteriología de primero a séptimo semestre de esta institución, encontrándose una frecuencia de portación de Staphylococcus aureus sensibles a meticilina (SASM) de 14,9 % [22], debido a que no se conoce la variación de la portación en estudiantes de Bacteriología a medida que estos avanzan en los semestres y están expuestos al ambiente del laboratorio clínico, el grupo investigador decidió evaluar la variación en el estado de portación nasal de esta bacteria en estudiantes del programa de Bacteriología de la Corporación Universitaria Rafael Núñez, que fueron definidos como portadores en el año 2010.…”

Section: Introductionunclassified

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Variación en el estado de portación nasal de Staphylococcus aureus sensibles a meticilina (SASM) en estudiantes de Bacteriología

Ferrer

Pérez

2013

Cienc. Salud virtual

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Introduction. Staphylococcus aureus colonizes to people mainly in nostril. Intermittent carriers of methicillin-sensible strains are in risk of acquire methicillin resistant S. aureus. There are different risk factors associated with methicillin-resistance S. aureus like hospitalization, skin lesions, antibiotic therapy, and so forth. Objective. To determine of variability in nasal carrier state of methicillin-susceptible Staphylococcus aureus in Bacteriology students'. Materials and Methods. Longitudinal analytical study. In 2012, samples of nostril were taken of 14 students previously classified like carriers (year 2010). S. aureus was identified by Kirby-Bauer method. A questionnaire was applied to identify risk facts associated to carriage state. Results of carriage from years 2010 and 2012 were compared. Results. In 2012, 5 carries of methicillin-susceptible Staphylococcus aureus strains were identified. Statistical association wasn't found between nasal carrier state and risk factors in 2010 and 2012 (p > 0.05). Conclusion. In 2012, were defined again 5 students like carriers that are reservoir and transmitting this strain.

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Staphylococcus aureus en Residentes de un Hogar de Ancianos de Cartagena

Abstract: Staphylococcus aureus nasal carriage found in this study was lower than that reported from other similar studies in other countries, taking into account that this is a population at risk for colonisation by this pathogen.

Cited by 8 publications

References 21 publications

Nasal carriage of Panton Valentine leukocidin-positive methicillin-resistant Staphylococcus aureus in healthy preschool children

Nasal carriage of Panton Valentine leukocidin-positive methicillin-resistant Staphylococcus aureus in healthy preschool children

Nasal and vaginal colonization of methicillin-resistant Staphylococcus aureus in pregnant women in Cartagena, Colombia

Variación en el estado de portación nasal de Staphylococcus aureus sensibles a meticilina (SASM) en estudiantes de Bacteriología

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