Staphylococcus aureus clumping factor B mediates biofilm formation in the absence of calcium

Abraham, Nabil M.; Jefferson, Kimberly K.

doi:10.1099/mic.0.057018-0

Cited by 71 publications

(70 citation statements)

References 29 publications

(34 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This is particularly relevant to MRSA as both hospital-associated and community-associated strains depend on proteins rather than polysaccharide for biofilm formation 103–105 . The CWA proteins that participate in biofilm formation are biofilm-associated protein (Bap) 106 , ClfB 107 , FnBPs 103,104 , SasC 108 , SasG 37,74 and protein A 109 . Bap, SasG (which is a homologue of the S. epidermidis CWA protein Aap) and SasC are present only in subsets of isolates, whereas the remaining CWA proteins are widely distributed.…”

Section: Cwa Proteins As Virulence Factorsmentioning

confidence: 99%

Adhesion, invasion and evasion: the many functions of the surface proteins of Staphylococcus aureus

et al. 2013

View full text Add to dashboard Cite

Staphylococcus aureus is an important opportunistic pathogen and persistently colonizes about 20% of the human population. Its surface is ‘decorated’ with proteins that are covalently anchored to the cell wall peptidoglycan. Structural and functional analysis has identified four distinct classes of surface proteins, of which microbial surface component recognizing adhesive matrix molecules (MSCRAMMs) are the largest class. These surface proteins have numerous functions, including adhesion to and invasion of host cells and tissues, evasion of immune responses and biofilm formation. Thus, cell wall-anchored proteins are essential virulence factors for the survival of S. aureus in the commensal state and during invasive infections, and targeting them with vaccines could combat S. aureus infections.

show abstract

Section: Cwa Proteins As Virulence Factorsmentioning

confidence: 99%

Adhesion, invasion and evasion: the many functions of the surface proteins of Staphylococcus aureus

et al. 2013

View full text Add to dashboard Cite

show abstract

“…Further studies revealed that the metalloprotease Aureolysin cleaves ClfB at two sites, resulting in the loss of fibrinogen binding (71, 72). ClfB promotes biofilm formation in vitro , and Aureolysin treatment disrupts ClfB-mediated biofilms, suggesting that Aureolysin might facilitate biofilm dispersal by processing ClfB (73). In vivo , ClfB is required for full virulence in a rat endocarditis model of infection, although the phenotype of the clfB mutant in this model was slight (74).…”

Section: Staphylococcal Adhesinsmentioning

confidence: 99%

The Staphylococcal Biofilm: Adhesins, Regulation, and Host Response

2016

View full text Add to dashboard Cite

The Staphylococci comprise a diverse genus of Gram-positive, non-motile commensal organisms that inhabit the skin and mucous membranes of humans and other mammals. In general, Staphylococci are benign members of the natural flora, but many species have the capacity to be opportunistic pathogens, mainly infecting individuals who have medical device implants or are otherwise immunocompromised. S. aureus and S. epidermidis are a major source of hospital-acquired infections and are the most common causes of surgical site infections and central line-associated bloodstream infections. The ability of Staphylococci to form biofilms in vivo makes them highly resistant to chemotherapeutics and leads to chronic diseases. These biofilm infections include osteomyelitis, endocarditis, medical device implants, and persistence in the cystic fibrosis lung. Here, we provide a comprehensive analysis of our current understanding of Staphylococcal biofilm formation, with an emphasis on adhesins and regulation, while also addressing how Staphylococcal biofilms interact with the immune system. On the whole, this review will provide a thorough picture of biofilm formation of the Staphylococcus genus and how this mode of growth impacts the host.

show abstract

“…However, it is now recognized that the accumulation of staphylococci can also be promoted by surface proteins in an ica -independent manner (particularly relevant for MRSA strains). These proteins are biofilm-associated proteins Bap [7], ClfB [10], FnBPs [11], SasC [12], SasG [13], and protein A [14]. ClfB, FnBPs, and protein A are widely distributed among strains.…”

Section: Introductionmentioning

confidence: 99%

Changes in the Expression of Biofilm-Associated Surface Proteins inStaphylococcus aureusFood-Environmental Isolates Subjected to Sublethal Concentrations of Disinfectants

Cincarova

Polanský

Babák

et al. 2016

BioMed Research International

View full text Add to dashboard Cite

Sublethal concentrations (sub-MICs) of certain disinfectants are no longer effective in removing biofilms from abiotic surfaces and can even promote the formation of biofilms. Bacterial cells can probably adapt to these low concentrations of disinfectants and defend themselves by way of biofilm formation. In this paper, we report on three Staphylococcus aureus biofilm formers (strong B+++, moderate B++, and weak B+) that were cultivated with sub-MICs of commonly used disinfectants, ethanol or chloramine T, and quantified using Syto9 green fluorogenic nucleic acid stain. We demonstrate that 1.25–2.5% ethanol and 2500 μg/mL chloramine T significantly enhanced S. aureus biofilm formation. To visualize differences in biofilm compactness between S. aureus biofilms in control medium, 1.25% ethanol, or 2500 μg/mL chloramine T, scanning electron microscopy was used. To describe changes in abundance of surface-exposed proteins in ethanol- or chloramine T-treated biofilms, surface proteins were prepared using a novel trypsin shaving approach and quantified after dimethyl labeling by LC-LTQ/Orbitrap MS. Our data show that some proteins with adhesive functions and others with cell maintenance functions and virulence factor EsxA were significantly upregulated by both treatments. In contrast, immunoglobulin-binding protein A was significantly downregulated for both disinfectants. Significant differences were observed in the effect of the two disinfectants on the expression of surface proteins including some adhesins, foldase protein PrsA, and two virulence factors.

show abstract

Staphylococcus aureus clumping factor B mediates biofilm formation in the absence of calcium

Cited by 71 publications

References 29 publications

Adhesion, invasion and evasion: the many functions of the surface proteins of Staphylococcus aureus

Adhesion, invasion and evasion: the many functions of the surface proteins of Staphylococcus aureus

The Staphylococcal Biofilm: Adhesins, Regulation, and Host Response

Changes in the Expression of Biofilm-Associated Surface Proteins inStaphylococcus aureusFood-Environmental Isolates Subjected to Sublethal Concentrations of Disinfectants

Contact Info

Product

Resources

About