Staphylococcus aureus Biofilm Destabilization by Tween-80 and Lung Surfactants to Overcome Biofilm-Imposed Drug Resistance

Maurya, Sarita; Gaur, Manish; Yadav, Awadh Bihari

doi:10.1007/s12010-023-04650-3

Cited by 1 publication

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References 28 publications

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“…After treatment, the medium was removed, and the wells were washed with 1× PBS, followed by 70% ethanol fixation incubation. After fixation, crystal violet staining was performed for 15 min, followed by adding 30% glacial acetic acid (v/v), and absorbance was measured at 595 nm using a multimode plate reader …”

Section: Methodsmentioning

confidence: 99%

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Evaluation of Drug-Loaded and Surface-Adsorbed DNase/Tween-80 Solid Lipid Nanoparticles against Staphylococcus aureus Biofilms

Maurya,

Gaur,

Akhtar

et al. 2024

ACS Appl. Bio Mater.

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The aim of this study was to explore the suitability of Tween-80 or DNase I adsorbed onto the surface of gentamicin-loaded solid lipid nanoparticles (SLNs) to disrupt Staphylococcus aureus biofilms in vitro. We hypothesized that surface-adsorbed DNase I or Tween-80 of SLNs will degrade the biofilm component, extracellular DNA (e-DNA), and extracellular matrix (ECM) of S. aureus biofilms. The SLNs loaded with drug (core) and surface-adsorbed disruptors (Tween-80 or DNase I) to deliver biofilm disruptors first at the site of action, which will help to break down the biofilm, and further drug release from the core will easily penetrate the biofilm and facilitate the killing of bacteria residing in S. aureus biofilms. The SLNs were synthesized by the double emulsion method; the size was 287.3 ± 7.4 nm for blank SLNs and 292.4 ± 2.36 nm for drug-loaded SLNs. The ζ-potential of blank SLNs was −25.6 ± 0.26 mV and that of drug-loaded SLNs was −13.16 ± 0.51 mV, respectively. The successful adsorption of DNase I or Tween-80 was confirmed by the activity of DNase I in blank surface-adsorbed SLNs and the change in the ζ-potential of SLNs after adsorbing DNase I or Tween-80. The surface morphology and size of the SLNs were further characterized using scanning electron microscopy. The encapsulation efficiency of the drug was 16.85 ± 0.84%. The compatibility of the drug with the excipient was confirmed by Fourier transform infrared spectroscopy and the degree of crystallinity was confirmed by X-ray diffraction (XRD) analysis. SLNs showed a sustained release of the drug up to 360 h. SLNs were easily taken up by A549 cells with minimal or no toxicity. The present study showed that Tween-80-or DNase I-adsorbed SLNs efficiently disrupt S. aureus biofilms and possess no or minimal toxicity against cells and red blood cells (RBCs).

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Section: Methodsmentioning

confidence: 99%

“…After fixation, crystal violet staining was performed for 15 min, followed by adding 30% glacial acetic acid (v/v), and absorbance was measured at 595 nm using a multimode plate reader. 24 2.14. Disruption of the S. aureus Biofilm after Nanoparticle Treatment, Qualitatively.…”

Section: Evaluation Of the Minimum Biofilm Inhibitory Concentration (...mentioning

confidence: 99%

Evaluation of Drug-Loaded and Surface-Adsorbed DNase/Tween-80 Solid Lipid Nanoparticles against Staphylococcus aureus Biofilms

Maurya,

Gaur,

Akhtar

et al. 2024

ACS Appl. Bio Mater.

Self Cite

View full text Add to dashboard Cite

show abstract

Staphylococcus aureus Biofilm Destabilization by Tween-80 and Lung Surfactants to Overcome Biofilm-Imposed Drug Resistance

Cited by 1 publication

References 28 publications

Evaluation of Drug-Loaded and Surface-Adsorbed DNase/Tween-80 Solid Lipid Nanoparticles against Staphylococcus aureus Biofilms

Evaluation of Drug-Loaded and Surface-Adsorbed DNase/Tween-80 Solid Lipid Nanoparticles against Staphylococcus aureus Biofilms

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