Staphylococcus aureus and MRSA Growth and Biofilm Formation after Treatment with Antibiotics and SeNPs

Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) is a dangerous pathogen resistant to β-lactam antibiotics. Due to its resistance, it is difficult to manage the infections caused by this strain. We examined this issue in terms of observation of the growth properties and ability to form biofilms in sensitive S. aureus and MRSA after the application of antibiotics (ATBs)—ampicillin, oxacillin and penicillin—and complexes of selenium nanoparticles (SeNPs) with these ATBs. The results suggest the strong inhibition effect of SeNPs in complexes with conventional ATBs. Using the impedance method, a higher disruption of biofilms was observed after the application of ATB complexes with SeNPs compared to the group exposed to ATBs without SeNPs. The biofilm formation was intensely inhibited (up to 99% ± 7% for S. aureus and up to 94% ± 4% for MRSA) after application of SeNPs in comparison with bacteria without antibacterial compounds whereas ATBs without SeNPs inhibited S. aureus up to 79% ± 5% and MRSA up to 16% ± 2% only. The obtained results provide a basis for the use of SeNPs as a tool for the treatment of bacterial infections, which can be complicated because of increasing resistance of bacteria to conventional ATB drugs.

Control of Biofilm Formation: Antibiotics and Beyond

“…A combination of antibiotics (ampicillin, oxacillin, and penicillin) with selenate NPs (SeNPs) was more effective (94%) in disrupting and inhibiting MRSA biofilms than the antibiotics alone (117).…”

Effect of antibiotics on biofilm inhibition and induction measured by real-time cell analysis

“…Therefore, sensitive, accurate, reproducible and fast methods are desirable for real-time monitoring of biofilms. In this regard, promising advances in impedance technology based on the ability of cells to impede the electrical current when adhered to MTP with gold electrodes have been developed in recent years (Junka et al 2012;Bruchmann et al 2015;Cihalova et al 2015).…”

“…The main purpose of this work was to study the effect of 10 antibiotics on biofilm formation dynamics in clinical isolates of S. aureus and S. epidermidis through an assay for monitoring biofilm development in real time using electronic impedance technology. Although real-time cell analysis (RTCA) assays were originally developed for monitoring eukaryotic cell lines status, and have been applied in a number of animal and human cell-based studies (Solly et al 2004), they have recently also been used for measuring bacterial biofilms and to study the effect of antimicrobial treatments on their growth (Junka et al 2012;Cihalova et al 2015). In the present study, we have applied electrode impedance measurements to test the therapeutic efficacy and effect on biofilm formation and growth of 10 antibiotics in clinically isolated bacterial strains.…”

Current Approaches for Exploration of Nanoparticles as Antibacterial Agents

“…Agar disk diffusion tests performed both according to the standardized protocols and with modifications are frequently used for susceptibility assessment and evaluation of antimicrobial activity of nanoparticles [109,[121][122][123][124]. Paper disc method has been employed as an alternative to standardized single disk method [112].…”