Staphylococcus and Linezolid Resistance in Iran

Shirvani, Fariba; Dashti, Anahita Sannai; Seifi, Kimia

doi:10.5812/pedinfect.12236

Cited by 5 publications

(5 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although in our study the lowest resistance was observed to linezolid (22.7%), this rate of resistance was high in comparison with many studies in Iran and other countries [23,28,29,30]. According to multinational and multicenter surveillance studies, more than 99% of coagulase-negative staphylococci and S. aureus clinical strains are sensitive to linezolid (31) [31]. The common mechanisms for linezolid resistance are mutations in the linezolid 23S rRNA binding site and acquisition of a plasmid-borne ribosomal methyltransferase gene called the cfr (chloramphenicol/florfenicol resistance) gene.…”

Section: Discussionmentioning

confidence: 48%

Investigation of SCCmec types I–IV in clinical isolates of methicillin-resistant coagulase-negative staphylococci in Ahvaz, Southwest Iran

et al. 2020

View full text Add to dashboard Cite

Today methicillin resistant coagulase-negative staphylococci (MR-CoNS) are important in terms of causing significant nosocomial infections. Besides, MR-CoNS are confirmed as the reservoir of SCCmec elements that carry mecA (methicillin-resistant) gene. Hence, the present study was designed to evaluate the susceptibility pattern, prevalence and diversity of SCCmec types I, II, III, and IV in MR-CoNS strains. In this cross-sectional study, 44 clinical isolates of MR-CoNS were identified using the cefoxitin disc method and further confirmation by polymerase chain reaction (PCR) amplification of the mecA gene. Antimicrobial susceptibility of isolates was investigated by disc diffusion. The identification of CoNS was done by amplification and sequencing of the tuf gene. Multiplex PCR method was done for the determination of SCCmec types. In the present study, the Staphylococcus epidermidis and Staphylococcus haemolyticus were the most predominant isolates with a prevalence of 45.4%. The highest resistance rates were observed against erythromycin (84.1%) and clindamycin (75%). Multiplex PCR revealed the SCCmec type I as the predominant type in the present study. Our study showed that there was no significant relationship between the presence of different types of SCCmec elements and resistance to antibiotics. The present study highlighted a frequent prevalence of MR-CoNS harboring SCCmec type genes in Ahvaz, southwest of Iran. Thus, the molecular typing and periodical monitoring of their drug resistance pattern should be considered in national stewardship programs to designing useful antibiotic prescription strategies.

show abstract

Section: Discussionmentioning

confidence: 48%

Investigation of SCCmec types I–IV in clinical isolates of methicillin-resistant coagulase-negative staphylococci in Ahvaz, Southwest Iran

et al. 2020

View full text Add to dashboard Cite

show abstract

“…The published reports of infections due to LRSA strains between the years 2001 and 2011 in different parts of world indicate a prevalence of 0.05% [31]. In studies conducted in different geographic areas of Iran, the LRSA strains have not been documented, except studies conducted in Tabriz and Mashhad [32]. Isolation of LRSA strains from important clinical specimens such as burn wound infection and diabetic foot ulcer, can be a serious threat due to the spread of these resistant strains in the hospitals.…”

Section: Discussionmentioning

confidence: 99%

Ability of biofilm production and molecular analysis of spa and ica genes among clinical isolates of methicillin-resistant Staphylococcus aureus

et al. 2020

View full text Add to dashboard Cite

Objective: This study aimed to evaluate the phenotypic and genotypic characterization of biofilm formation and spa and ica genes among clinical isolates of methicillin-resistant Staphylococcus aureus. Result: This cross-sectional study was performed on 146 Staphylococcus aureus isolates from hospitalized patients in Isfahan Province Hospitals. MRSA isolates were confirmed using disk diffusion test with oxacillin disk and amplification of mecA gene by PCR assays. Ability of biofilm production was evaluated targeting the icaA and icaD genes. Of 146 Staphylococcus aureus isolates, 24 (16.4%) carried mecA genes and identified as MRSA strains. Strong ability of biofilm production was seen among 76.02% (111/146) S. aureus isolates and 87.5% (21/24) MRSA strains, respectively. Also, 75.0% (18/24) MRSA isolates carried icaA and icaD was not detected in these strains. Analysis of spa gene showed 70.83% (17/24) MRSA strains were spa positive. From which 14 and 3 strains identified with one band (150, 270, 300, 360, 400 bp) and two bands (150-300 bp), respectively. According to data obtained, the prevalence of MRSA isolates from Isfahan Province Hospitals is relatively high and a remarkable percentage of them show strong power in biofilm production. Also analysis of spa gene showed a fairly large diversity among MRSA strains.

show abstract

“…Linezolid is effective against more than 98% of Staphylococcus infections, with resistance detected in only 0.05% of S. aureus infections. In many studies conducted in Iran, resistance to linezolid was either not reported or was very low (25). Studies analyzing the resistance of Staphylococcus isolates to linezolid in various countries found that the United States, Canada, and European countries had higher levels of resistance, while African and Asian countries reported the lowest (0.1%) resistance among MRSA strains (26).…”

Section: Discussionmentioning

confidence: 99%

New Sequence Types of Staphylococcus aureus Strains Isolated from Hospitals and Community Settings in Southern Iran

Shokoohizadeh,

Dehghani,

NamordiZadeh

et al. 2024

Jundishapur J Microbiol

View full text Add to dashboard Cite

Background: Staphylococcus aureus is a significant bacterial pathogen globally recognized as the primary cause of numerous uncomplicated skin infections and severe invasive infections. The emergence of methicillin-resistant S. aureus (MRSA) poses a serious threat, leading to severe infections in both hospitals and community settings. Objectives: The aim of this study was to identify antibiotic resistance patterns and perform molecular classification of S. aureus strains isolated from both hospital and community settings in southern Iran. Methods: In this cross-sectional study conducted in Bandar Abbas between 2020 and 2021, a total of 156 clinical strains of S. aureus were collected. Antibiotic susceptibility was determined using the disk-diffusion agar method. The presence of the pvl gene, Sccmec types, and Agr group was identified through PCR analysis. Additionally, Multilocus sequence typing (MLST) was performed on selected isolates. Results: The study identified 156 strains, with 79 obtained from inpatients and 77 from outpatients, sourced from clinical samples. Among these isolates, 70 (44.8%) were classified as MRSA. The highest resistance was noted against azithromycin (83%), while the lowest resistance was observed for linezolid (5%) and gentamicin (7%). The presence of the pvl gene was detected in isolates from both hospital and community sources. Significant differences were noted in the occurrence of agr I and agr III genes between hospital and community isolates. Sccmec III was more predominant than other SCCmec types. Furthermore, MLST analysis revealed the presence of five distinct novel sequence types (STs): ST8634, ST8640, ST8650, ST8152, and ST8153. Conclusions: The findings indicate that the potential spread of hospital-acquired S. aureus strains to the community and vice versa poses a significant public health risk. This underscores the urgent need for robust infection control strategies and the identification of potential environmental and hospital sources of resistant strains, particularly MRSA strains.

show abstract

Staphylococcus and Linezolid Resistance in Iran

Cited by 5 publications

References 15 publications

Investigation of SCCmec types I–IV in clinical isolates of methicillin-resistant coagulase-negative staphylococci in Ahvaz, Southwest Iran

Investigation of SCCmec types I–IV in clinical isolates of methicillin-resistant coagulase-negative staphylococci in Ahvaz, Southwest Iran

Ability of biofilm production and molecular analysis of spa and ica genes among clinical isolates of methicillin-resistant Staphylococcus aureus

New Sequence Types of Staphylococcus aureus Strains Isolated from Hospitals and Community Settings in Southern Iran

Contact Info

Product

Resources

About