Staphylococci and implant surfaces: a review

Harris, Llinos G.; Richards, R. Geoff

doi:10.1016/j.injury.2006.04.003

Cited by 338 publications

(279 citation statements)

References 124 publications

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“…However, overuse of antibiotics in medicine promoted the evolution of some species of bacteria to infer resistance. These include the Methicillin resistant S.aureus (MRSA) and S. epidermidis (MRSE) strains which are responsible for a considerable amount of hospital acquired infections [33][34][35]. The primary reason for increased usage of antibiotics in implant related infections is that bacteria change their biological behavior when adhering to implant surfaces [2].…”

Section: Resultsmentioning

confidence: 99%

Comparison of antibacterial properties of commercial bone cements and fillers with a zinc-based glass polyalkenoate cement

2010

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Section: Resultsmentioning

confidence: 99%

Comparison of antibacterial properties of commercial bone cements and fillers with a zinc-based glass polyalkenoate cement

2010

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“…Usually, antibiotics are the main tools to fight infection. A valuable method to avoid infection is to reduce bacterial adhesion on implant by the control of the fixation surfaces topography and chemistry (Harris and Richards, 2006). The use of degradable polymers has been proposed for the coating of titanium plates and the local release of loaded antibiotic and antiseptic in a controlled fashion.…”

Section: Eglin and M Alinimentioning

confidence: 99%

Degradable polymeric materials for osteosynthesis: Tutorial

Eglin

Alini²

2008

eCM

110

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This report summarizes the state of the art and recent developments and advances in the use of degradable polymers devices for osteosynthesis. The current generation of biodegradable polymeric implants for bone repair utilising designs copied from metal implants, originates from the concept that devices should be supportive and as "inert" substitute to bone tissue. Today degradable polymeric devices for osteosynthesis are successful in low or mild load bearing applications. However, the lack of carefully controlled randomized prospective trials that document their efficacy in treating a particular fracture pattern is still an issue. Then, the choice between degradable and non-degradable devices must be carefully weighed and depends on many factors such as the patient age and condition, the type of fracture, the risk of infection, etc. The improvement of the biodegradable devices mechanical properties and their degradation behaviour will have to be achieved to broaden their use. The next generation of biodegradable implants will probably see the implementation of the recent gained knowledge in cellmaterial interactions and cells therapy, with a better control of the spatial and temporal interfaces between the material and the surrounding bone tissue.

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“…Device-related staphylococcal infections are difficult to eradicate and treat clinically (5) because bacteria in the biofilm are protected from antimicrobial agents and the host immune system (6). Prolonged antibiotic therapy and device removal can be required to resolve the infection (5).…”

mentioning

confidence: 99%

Staphylococcal biofilm-forming protein has a contiguous rod-like structure

Gruszka

Wojdyla

Bingham

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

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Staphylococcus aureus and Staphylococcus epidermidis form communities (called biofilms) on inserted medical devices, leading to infections that affect many millions of patients worldwide and cause substantial morbidity and mortality. As biofilms are resistant to antibiotics, device removal is often required to resolve the infection. Thus, there is a need for new therapeutic strategies and molecular data that might assist their development. Surface proteins S. aureus surface protein G (SasG) and accumulation-associated protein ( S. epidermidis ) promote biofilm formation through their “B” regions. B regions contain tandemly arrayed G5 domains interspersed with approximately 50 residue sequences (herein called E) and have been proposed to mediate intercellular accumulation through Zn 2+ -mediated homodimerization. Although E regions are predicted to be unstructured, SasG and accumulation-associated protein form extended fibrils on the bacterial surface. Here we report structures of E–G5 and G5–E–G5 from SasG and biophysical characteristics of single and multidomain fragments. E sequences fold cooperatively and form interlocking interfaces with G5 domains in a head-to-tail fashion, resulting in a contiguous, elongated, monomeric structure. E and G5 domains lack a compact hydrophobic core, and yet G5 domain and multidomain constructs have thermodynamic stabilities only slightly lower than globular proteins of similar size. Zn 2+ does not cause SasG domains to form dimers. The work reveals a paradigm for formation of fibrils on the 100-nm scale and suggests that biofilm accumulation occurs through a mechanism distinct from the “zinc zipper.” Finally, formation of two domains by each repeat (as in SasG) might reduce misfolding in proteins when the tandem arrangement of highly similar sequences is advantageous.

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Staphylococci and implant surfaces: a review

Cited by 338 publications

References 124 publications

Comparison of antibacterial properties of commercial bone cements and fillers with a zinc-based glass polyalkenoate cement

Comparison of antibacterial properties of commercial bone cements and fillers with a zinc-based glass polyalkenoate cement

Degradable polymeric materials for osteosynthesis: Tutorial

Staphylococcal biofilm-forming protein has a contiguous rod-like structure

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