Staphylococcal toxin-induced T cell proliferation in atopic eczema correlates with increased use of superantigen-reactive V<i>β</i>-chains in cutaneous lymphocyte-associated antigen (CLA)-positive lymphocytes

Davison, S C; Allen, Michael; Vaughan, R. W.; Barker, Jonathan

doi:10.1046/j.1365-2249.2000.01270.x

Cited by 34 publications

(34 citation statements)

References 20 publications

(26 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Briefly, PBMCs (2 ϫ 10 6 cells/ml) were stimulated with TSST-1 (10 ng/ml) in the presence or the absence of anisodamine (50 g/ml) for 3 days. This was followed by a further incubation with rIL-2 to allow reexpression of the T-cell receptor (3,9). The cells were then stained with FITC-V␤2 and PE-CD3 and examined by two-color flow cytometry with an EPICS XL flow cytometer (Beckman Coulter).…”

mentioning

confidence: 99%

Specific Inhibitory Action of Anisodamine against a Staphylococcal Superantigenic Toxin, Toxic Shock Syndrome Toxin 1 (TSST-1), Leading to Down-Regulation of Cytokine Production and Blocking of TSST-1 Toxicity in Mice

Nakagawa

Kushiya

Taneike

et al. 2005

Clin Vaccine Immunol

View full text Add to dashboard Cite

Toxic shock syndrome toxin 1 (TSST-1), produced by Staphylococcus aureus (including methicillin-resistant S. aureus), is a superantigenic toxin responsible for toxic shock syndrome as well as neonatal TSS-like exanthematous disease. TSST-1 exhibits its deleterious effects by leading to the abnormal proliferation of, e.g., V␤2؉ T cells and overproduction of proinflammatory cytokines. In the present study we examined the inhibitory effect of a Chinese herbal extract, anisodamine, on TSST-1 using human peripheral blood mononuclear cells (PBMCs). Anisodamine inhibited the production of proinflammatory cytokines better than interleukin-10 (an anti-inflammatory cytokine). The inhibitory effect of anisodamine was greater than that of any tropane alkaloid examined. Anisodamine acted directly on both monocytes and T cells in human PBMCs, and the effect was confirmed at the transcriptional level. Inhibition of NF-B activation was also demonstrated. In contrast, no significant inhibition of V␤2 ؉ T-cell proliferation was observed. In mice injected with TSST-1, anisodamine treatment significantly decreased serum proinflammatory cytokine levels and prevented TSST-1-induced death. These results suggest that anisodamine specifically acts against the production of cytokines (inflammatory cytokines in particular) and not against V␤2 ؉ T-cell proliferation and that anisodamine may have a beneficial effect on TSST-1-associated disease.

show abstract

mentioning

confidence: 99%

Specific Inhibitory Action of Anisodamine against a Staphylococcal Superantigenic Toxin, Toxic Shock Syndrome Toxin 1 (TSST-1), Leading to Down-Regulation of Cytokine Production and Blocking of TSST-1 Toxicity in Mice

Nakagawa

Kushiya

Taneike

et al. 2005

Clin Vaccine Immunol

View full text Add to dashboard Cite

show abstract

“…All these superantigens can amplify the expression of skin-homing receptor (such as CLA) on T cells and induce the appearance of skewed T cell clones [26,27,32]. For example, toxic shock toxin 1 can selectively stimulate TCR-Vβ2 bearing cells, staphylococcal enterotoxin C might bind both Vβ5.1 and Vβ13.1, staphylococcal enterotoxin B and staphylococcal enterotoxin C can excite Vβ17 and staphylococcal enterotoxin E can recognize Vβ5.1 [67,68,69,70]. A skewed usage of the TCR-Vβ subfamilies 2, 5.1 and 13.1 in the circulating T cell clone of SS patients has been reported [37,71].…”

Section: Discussionmentioning

confidence: 99%

Specific IgE toward Allergenic Molecules Is a New Prognostic Marker in Patients with Sézary Syndrome

Scala¹,

Abeni²,

Palazzo³

et al. 2011

Int Arch Allergy Immunol

View full text Add to dashboard Cite

Background: Sézary syndrome (SS) is the aggressive leukemic form of cutaneous T cell lymphoma characterized by erythroderma, the presence of a malignant circulating T memory cells with skin homing potential, and the ability to produce a variety of Th2 soluble factors, such as IL-4 and IL-5. We measured total and specific IgE in SS patients as a further parameter of a Th2-skewed immune system, and studied their clinical impact. Methods: Specific IgE production in a cohort of 55 SS patients was evaluated by the molecule-based ISAC microarray system. We then evaluated survival times and the hazard ratios in this cohort by Kaplan-Meier and Cox methods. Results: Twenty-four (43.6%) SS patients had specific IgE to both environmental and food allergens. For survival analysis, patients found positive to at least one allergen were defined as IgE+. By comparing IgE+ versus IgE– we found a significant difference in the median survival times, 2.9 versus 8.9 years (p < 0.001). Conversely, no survival difference could be observed when total IgE levels were considered. IgE+ patients had higher levels of CD60+CD49–CD4+ T cells, which also represent another worse prognostic index recently identified. Conclusion: SS patients had specific IgE to both environmental and food allergens. IgE+ SS patients had a significant lower survival rate. High levels of CD60–CD49+CD4+ T cells associated with an IgE– phenotype allow the identification of a restricted group of long survivor SS patients. Therefore, specific measurement of IgE seems to be useful in discriminating survival.

show abstract

“…47,48,52 For example, TSST-1 may stimulate selectively TCR-Vβ2-bearing cells, SE-C may bind both Vβ5.1 and Vβ13.1, SE-B and SE-C Vβ17 and SE-E Vβ5.1. [53][54][55][56] A repeated super-antigenic stimulation of the TCR would cause activationinduced cell death. 57 Aberrant over-expression of CD60, which confers anti-apoptotic properties, could explain why repetitive stimulation by a super-antigen may eventually result in T-cell oligoclonality.…”

Section: Discussionmentioning

confidence: 99%

The role of 9-O-acetylated ganglioside D3 (CD60) and 4 1 (CD49d) expression in predicting the survival of patients with Sezary syndrome

Scala

Abeni²,

Pomponi³

et al. 2010

Haematologica

View full text Add to dashboard Cite

BackgroundSézary syndrome is a rare and very aggressive leukemic variant of cutaneous T-cell lymphoma characterized by extensive skin involvement and a malignant circulating CD4 + T-cell clone which homes to the skin, over-expresses CD60, and lacks CD7, CD26 and CD49d. So far prognostic markers in this disease are limited to treatment with systemic steroids, age, serum lactate dehydrogenase, and a white blood cell count of 20¥10 9 /L or higher: no other biological marker with prognostic value, especially related to malignant cells, has been described. Design and MethodsWe used flow activated cell sorting analysis to compare the distribution of the T-cell receptor-Vβ repertoire and several surface molecules (CD7, CD26, CD49d and CD60) within the circulating CD4 + T-cell population in 62 patients with Sézary syndrome, 180 with mycosis fungoides, 6 with B-cell lymphomas, and 19 with chronic eczema. We calculated the 5-year overall survival of patients with Sézary syndrome after first hospital admission using Kaplan-Meier product-limit estimates and hazard ratios from the Cox proportional hazards model. ResultsWe found that both higher number of CD60 + and lower number of CD49d + cells within circulating CD4 + T cells at disease presentation were significantly associated with a lower probability of survival. An exceedingly high risk of death was observed for patients with a combination of a high proportion of CD4 /L) (hazard ratio = 12.303, 95% confidence interval 1.5-95.9; P<0.02). In addition, a skewed usage of T-cell receptor-Vβ subfamilies was observed in the circulating T-cell clone for 61.9% of all patients with Sézary syndrome, T-cell receptor-Vβ 2 and 5.1 subfamilies being the most frequently represented (42.8%), followed by T-cell receptor-Vβ 12 and 13.1. ConclusionsIn this study we showed that up-regulation of CD60 and down-regulation of CD49d on circulating CD4 + T cells are two useful markers for predicting a very poor outcome in patients with Sézary syndrome.Key words: TCR-Vβ repertoire, CD60, CD49d, survival rate, Sézary syndrome. Haematologica 2010;95(11):1905-1912. doi:10.3324/haematol.2010 This is an open-access paper.The role of 9-O-acetylated ganglioside D3 (CD60) and α4β1 (CD49d) expression in predicting the survival of patients with Sézary syndrome

show abstract

Staphylococcal toxin-induced T cell proliferation in atopic eczema correlates with increased use of superantigen-reactive Vβ-chains in cutaneous lymphocyte-associated antigen (CLA)-positive lymphocytes

Cited by 34 publications

References 20 publications

Specific Inhibitory Action of Anisodamine against a Staphylococcal Superantigenic Toxin, Toxic Shock Syndrome Toxin 1 (TSST-1), Leading to Down-Regulation of Cytokine Production and Blocking of TSST-1 Toxicity in Mice

Specific Inhibitory Action of Anisodamine against a Staphylococcal Superantigenic Toxin, Toxic Shock Syndrome Toxin 1 (TSST-1), Leading to Down-Regulation of Cytokine Production and Blocking of TSST-1 Toxicity in Mice

Specific IgE toward Allergenic Molecules Is a New Prognostic Marker in Patients with Sézary Syndrome

The role of 9-O-acetylated ganglioside D3 (CD60) and 4 1 (CD49d) expression in predicting the survival of patients with Sezary syndrome

Contact Info

Product

Resources

About